Bielefeld, Germany
Bielefeld, Germany

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Volmer M.,Bielefeld University | Northoff S.,TeutoCell AG | Scholz S.,Bielefeld University | Thute T.,Bielefeld University | And 3 more authors.
Biotechnology Letters | Year: 2011

A new method for sampling suspended animal cells by fast filtration is presented that allows rapid quenching of cellular metabolism and efficient separation of the cells from culture medium. Compared to sampling with a microstructure heat exchanger or centrifugation without prior quenching, the adenylate energy charge and the measured concentrations especially of metabolites with a high turnover rate or of metabolites early in metabolic pathways were substantially higher. No leakage of ATP from the cells was observed when using iso-osmotic NaCl solution in the washing step. The combination of fast filtration and cold methanol extraction is therefore suitable for intracellular metabolomic studies of suspended animal cell cultures and superior to other methods currently applied. © 2010 Springer Science+Business Media B.V.

Northoff S.,TeutoCell AG | Heinrich C.,TeutoCell AG | Buntemeyer H.,TeutoCell AG | Hubel T.,Miltenyi Biotec GmbH | Schroder B.,Miltenyi Biotec GmbH
BioSpektrum | Year: 2012

The development of culture media and supplements for fed-batch processes is one of the most complex steps in cell culture process development. We have developed chemically defined and animal com - ponent-free media and supplements for culturing Chinese Hamster Ovary cells (CHO) and hybridoma cells, improving the production of recombinant proteins. © Springer-Verlag 2012.

Jordan I.,ProBioGen AG | Northoff S.,TeutoCell AG | Thiele M.,ProBioGen AG | Hartmann S.,ProBioGen AG | And 10 more authors.
Biologicals | Year: 2011

Highly attenuated poxviruses are promising vectors for protective and therapeutic vaccines. These vectors do not replicate in human cells and can therefore be safely given even to immunocompromised recipients. They can accomodate very large inserts and provide strong stimulation of the immune system against the vectored antigen. Disadvantages include that very high numbers of infectious units are required per dose for full efficacy. Because they are difficult to produce, improved cellular substrates and processes are urgently needed to facilitate programs intended to reach a large number of vaccinees. We have developed a fully scalable and very efficient chemically-defined production process for modified vaccinia Ankara (MVA), canarypox (CNPV, strain ALVAC) and fowlpox viruses (FPV) based on a continuous cell line. © 2010 The International Association for Biologicals.

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