Korda M.,Ternopil State Medical University |
Meretskyi V.,Ternopil State Medical University |
Meretska I.,Ternopil State Medical University
Georgian medical news | Year: 2017
This article describes the immunological events and investigates important mediators of the inflammatory immune response after experimental cranial trauma combined with type I diabetes. It was shown that the rats with induced diabetes and traumatic brain injury suffered more profound damage to the immune system that was not restored back to the basal level within 14 days. Proinflammatory cytokine concentrations increased, while anti-inflammatory cytokine concentrations decreased by day 14, increasing risk for systemic inflammatory response syndrome and multi-organ failure. Factors responsible for humoral immunity and cell-mediated immunity were consistently lower by day 14, which might make diabetic rats more susceptible to infections compared to rats with brain injury alone.
Logoyda L.S.,Ternopil State Medical University |
Polyauk O.B.,Ternopil State Medical University |
Korobko D.B.,Ternopil State Medical University |
Chir M.B.,Ministry of Internal Affairs of Ukraine in Ternopil Region
Asian Journal of Pharmaceutical Research and Health Care | Year: 2016
Methods for the quantitative determination of loratadine tablets by differential spectrophotometry and photo-colorimetry have been developed. During the procedure parameters (range of application methods, stability, specificity, robasnist, convergence, internal laboratory accuracy (precision), accuracy, reproducibility) have been considered. The results indicate that the method photo-colorimetry has not met modern criteria for analytical methods for quantitative determination of parameters such as stability, accuracy and linearity, so other validation parameters (robasnist, convergence and inter-laboratory precision) have been determined only by spectrophotometric methods. It is found that the technique of quantitative determination of loratadine by differential spectrophotometry is accurate, correct, reproducible and linear, to determine its suitability for use in pharmaceutical analysis. © 2016, Journal of Pharmaceutical Research and Health Care. All rights reserved.
Jun S.,Wuhan University of Science and Technology |
Kochan O.,Lviv Polytechnic |
Levkiv M.,Ternopil State Medical University
2017 11th International Conference on Measurement, MEASUREMENT 2017 - Proceedings | Year: 2017
The structure of the simplified metrological software test (MST) for modeling of thermocouple (TC) errors during operation are considered in the paper. Also the method of MST connection to a temperature measuring system in order to determine the error of a TC is presented. The studies of the dependence of TC error on interference magnitude in the modes of single measurements and averaging was carried out. © 2017 Institute of Measurement Science SAS.
Yarema N.M.,Ternopil State Medical University |
Yarema N.M.,Russian Academy of Sciences
Voprosy Pitaniia | Year: 2017
The use of additional treatment methods in inflammatory joints disease therapy is very important. But the main principles of diet therapy for patients with rheumatoid joint inflammation and reactive arthritis and possibility of focused impact on disease activity by means of alimentary factors have not still been formed out. The aim of the investigation was to study the effect of diet therapy including ω-3 polyunsaturated fatty acids (PUFAs) on joint syndrome evidence and on bone turnover markers of children with inflammatory joint diseases. With parents' agreement children aged 5-16 hospitalized with inflammatory joint diseases (53 with juvenile rheumatoid arthritis and 35 with reactive arthritis) were enrolled in this research. According to the treatment mode 2 subgroups were separated in each group: the first subgroup underwent backbone therapy, the second - backbone therapy along with ω-3 PUFAs (cod liver oil 1000 mg containing 115 mg of docosahexaenoic and 23 mgof eicosapentaenoic acids). Children aged 3-5 years received 1 capsule 2 times a day, over 6 years old - 1 capsule 3 times per day with meals for 3 months. Disease activity, joint syndrome evidence (counting of joint pain, Ritchie index, number of inflammatory joints, morning stiffness duration) and biochemical values of connective tissue metabolism were estimated while being introduced into research and at the end of treatment. More apparent improvement of joint syndrome indexes at the end of supervision was diagnosed in the groups undergoing backbone therapy along with co-3 PUFAs. Statistically significant (p<0.05) reduce of morning stiffness time by 3 fold vs 2 fold in children treated with basic therapy, reduce of joint index by 2.9-5.7 fold vs 2.0-3.8 fold, the number of inflammatory joints by 4.5-5.8 fold vs 2.0-2.3 fold, blood serum level of hydroxyl proline and antibodies to rumalon were observed in main groups of patients. Disease activity index DAS 4 decreased in the group undergoing backbone therapy by 0.44 (p<0.05) and in the group undergoing modified therapy - by 1.20 (p<0.05). No adverse effects of PUFAs have been observed. It was concluded that ω-3 PUFAs increased the action of basic therapy favoring advances in inflammatory process activity control, provided decrease of non-steroidal antiinflammatory drugs (NSAID) intake and proved to be an important supplement in diet therapy of children inflammatory joints diseases.
Halyna P.,Ternopil State Medical University |
Iryna S.,Ternopil State Medical University
Signa Vitae | Year: 2017
Introduction. Nitric oxide (NO) is an important diagnostic marker and mediator in the inflammatory process, which plays a key role in the mechanism of programmed cell death, thus, forming the basis of many pathological diseases. Methods. The study involved 73 newborns with pneumonia (moderate severity in 44 neonates (group 1), severe pneumonia in 29 (group 2)). The intensity of neutrophil apoptosis and necrosis was determined by flow cytometry, whereas nitric oxide metabolites were measured by spectrophotometry. Results. The level of nitric oxide metabolites (NO2+NO3) in newborns with pneumonia was higher than in healthy children (16.93 (15.82; 17.79) μmol/ml) and correlated with disease severity (in group 1 – 22.65 (21.42; 23.40) μmol/ml in group 2 – 26.82 (25.81; 27.91) μmol/ml). The level of NO3 increased moderately, while NO2 generation was more intense, exceeding control indexes in both groups (рc- 1<0.001; рc-2<0.001; р1-2<0.001). The occurrence of intensive neutrophil apoptosis was revealed in newborns with pneumonia of moderate severity (рc- 1<0.001), while necrosis prevailed in severe pneumonia (рc-2<0.001). Inverse correlation (R=-0.63; р<0.05) was found between the level of nitric oxide metabolites and neutrophil apoptosis; and direct correlation (R=0.68; р<0.05) was revealed between NO metabolites and neutrophil necrosis indices. Conclusions. Increased generation of nitric oxide metabolites, that directly correlated with disease severity in newborns with pneumonia, was found. NO2 has multidirectional effects on neutrophil apoptosis and necrosis, leading to toxic accumulation of neutrophils in the organism, thus enhancing the inflammatory and intoxication process that impact disease severity. © 2017, Pharmamed Mado Ltd., All Rights Reserved.
Kvach Y.,Ukrainian Academy of Sciences |
Drobiniak O.,Ukrainian Academy of Sciences |
Kutsokon Y.,Ukrainian Academy of Sciences |
Hoch I.,Ternopil State Medical University
Knowledge and Management of Aquatic Ecosystems | Year: 2013
The parasites of the Asian invasive fish, Chinese sleeper Perccottus glenii, were studied in 6 localities in different parts of Ukraine. In total, 15 taxa of parasites were registered; among them were 1 species of Microsporidia, 5 species of ciliates, 2 species of cestodes, 2 species of trematodes, 2 species of nematodes, 1 species of acanthocephalan, 1 species of parasitic crustacean and 1 mollusk (glochidia). The invasive Chinese sleeper is included as a paratenic host in the life cycle of the parasites of indigenous reptiles in Europe. The non-indigenous cestode Nippotaenia mogurndae occurred in the intestine of the Chinese sleeper from the Ivachiv Reservoir (Dniester River basin). This cestode is recorded for Ukrainian fauna for the first time. In addition, 3 species of parasites were recorded in the Chinese sleeper for the first time: Nicolla skrjabini, Cosmocephalus obvelatus and Pomphorhynchus laevis. We note the low similarity among the different localities and the low parasite richness, that suggest that the parasite fauna of the Chinese sleeper in Ukraine is in transition. © ONEMA, 2013.
Shevchuk O.O.,Ternopil State Medical University |
Posokhova E.A.,Ternopil State Medical University |
Sakhno L.A.,R.E. Kavetsky Institute of Experimental Pathology |
Nikolaev V.G.,R.E. Kavetsky Institute of Experimental Pathology
Experimental Oncology | Year: 2012
Anthracyclines play an important role in treatment of variety types of cancer due to their high effectiveness and broad spectrum of activity. However, a major limitation of their use is the dose-limiting cardiotoxicity. The inability to predict and prevent anthracycline cardiotoxicity is in part due to the fact that the molecular and cellular mechanisms remain controversial and incompletely understood. This review focuses on the biochemical basis of the anthracyclines toxic cardiac effects and pharmacological measures to their treatment and preventing. We describe the theoretical substantiation of the enterosorption abilities for diminishing of cardiac damage. Copyright © Experimental Oncology, 2012.
Nedoshytko Kh.Yu.,Ternopil State Medical University
Ukrain'skyi Biokhimichnyi Zhurnal | Year: 2013
The state of antioxidant system and fatty acid composition of lipids in the liver tissues of rats of different sex at the ethanol and tetracycline action and at the influence of biologically active additives (BAA) Alpha + Omega at a dose of 0.5 mgkg b.w. per os was investigated. It was found that the contet of lipid peroxidation products in the liver was increased at the action of 40 % ethanol at a dose of 7 mlkg b.w. per os and tetracycline - 500 mgkg and more profound at their joint using. However, the content of diene conjugates was stronger increased in the liver of females at the action of ethanol, while in the liver of males at the action of tetracycline ( < 0.05). It was shown that the application of the investigated compounds led to the reduction of an antioxidant defense system activity of males and females liver, as evidenced by the decrease of superoxide dismutase activity by 46 and 43% and reduction of glutathione content by 39 and 38% ( < 0.05). The activity of alanineaminotransferase, aspartateaminotransferase and alkalinephosphatase was increased in the liver of males and females under the influence of ethanol and tetracycline and more profound at their joint usage ( < 0.05). It was established that ethanol and tetracycline unidirectionally changed fatty acid composition of total lipids of rat liver, but at the ethanol action the changes were more expressed in females while at the tetracycline action in males. The application during 14 days of BAA Alpha + Omega to male and female rats with an acute tetracycline damage at subacute ethanol action led to partial normalization of prooxidantantioxidant system and the relative content of total lipids fatty acids of the liver of both sexes animals.
Nadashkevich O.,Lviv National Medical University |
Masyk O.,Ternopil State Medical University
Clinical Rheumatology | Year: 2011
The aim of this study was to translate and adapt the original English-language versions of the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and the Bath Ankylosing Spondylitis Functional Index (BASFI) into Ukrainian and validate the instruments by administering them to Ukrainian patients with ankylosing spondylitis (AS). After consensual translation, cultural adaptation, and back-translation into English, the Ukrainian versions of BASDAI and BASFI were transformed into numerical rating scales and tested on 87 Ukrainian AS patients meeting the modified 1984 New York criteria for AS. The Ukrainian versions were easily understood by the patients and displayed high levels of internal consistency and good reliability. The BASDAI (Ukrainian version) showed significant correlation with the duration of morning stiffness and the Maastricht Ankylosing Spondylitis Enthesitis Score (MASES) and weak correlation with CRP. The BASFI (Ukrainian version) showed significant correlations with Schober's test, occiput-to-wall distance, and chest expansion measure. Both instruments were sensitive to modifications in the treatment regimen. Accordingly, the Ukrainian-language versions of BASDAI and BASFI reported here can be used to monitor the clinical status of Ukrainian patients with AS. © 2011 Clinical Rheumatology.
Shidlovsky A.V.,Ternopil State Medical University
Novosti Khirurgii | Year: 2015
Objectives. To study the possibility of using the laser interstitial thermotherapy (LITT) in the treatment of the nodular euthyroid goiter and factors influencing its efficacy. Methods. Using LITT 134 patients with benign solitary thyroid nodes had been treated. Single silica fiber, the output power of 2,8-3,2 Watts, continuous mode of radiation, wavelength of 1060 nm, continuous ultrasound guidance had been used. Changes in the structure and volume of nodules according to the ultrasonography data were studied within the 1th day, in 2 weeks, 1, 3, 6, 9, 12 and 15 months, respectively. The gland function and level of antibodies to thyroid peroxidase were determined by standard tests prior treatment, 1 and 6 months after treatment. The long-term results were studied three years afterwards. Results. After 24 hours the volume of nodes increased on the average to 12,21,21% (p 0,05). In subsequent periods of observation the sizes of nodes reduced. The final assessment of the complete replacement of nodes on the scar tissue occurred in 128 (95,5%) cases, and the volume of nodes reduced from 3,02 cm3 (range 1,2-10,2) up to 0,57 (range of 0,1-1,3; p0,001). Unsatisfactory result was registered in 6 (4,5%) cases without any complications. At control survey (36 months after) the volume of scar tissue remained unchanged and the parameters of thyrotropic hormone, antibodies to thyroid peroxidase were within the normal range in 68 cured patients. Conclusion. Laser thermotherapy is considered to be a highly effective, minimally invasive, safe and inexpensive method of outpatient treatment of the nodular goiter. Its effectiveness depends on the echogenicity and volume of nodes. It can be used as an independent method of treatment in the period from clinically significant node up to the development of the compression syndrome. In some cases laser interstitial thermo-therapy lets to avoid possible surgical treatment.