Okazaki, Japan
Okazaki, Japan

Time filter

Source Type

Kimoto T.,Japan National Institute of Materials Science | Arai Y.,Terabase Inc. | Nagayama K.,National Institute for Physiological science
Applied Surface Science | Year: 2012

We developed vacuum deposition equipment for coating a photoemissive material on a cathode tip for TEM in high vacuum. For practical reasons, we firstly examined whether Cs reacts chemically with Sb at low temperature. Photoelectrons were emitted from the as-deposited layers of Sb, Cs and Sb deposited on the cathode tip at -20°C less than 0.5 h after the second Sb deposition. Since the quantum efficiency calculated from the photocurrent was much more than 10 4 times as large as that of substrate materials, we concluded that Cs 3Sb film was formed by the reaction of Cs and Sb at -20°C. The quantum efficiency of the Cs 3Sb 2 h after the second Sb deposition was 0.021-0.029%, but it decreased rapidly with time at temperatures of both about -20°C and about 10.5°C at the vacuum level of 1.2-1.4 × 10 -7 Pa. Photoelectrons were successfully detected from the Cs 3Sb coated on a small area of 70 μm in diameter over the cathode tip whose diameter was 0.2 mm. It was also determined that photoelectrons emitted from Cs 3Sb decreased with anode voltage from 0.1 to 0.4 kV, but increased from 0.4 to 7 kV. © 2012 Elsevier B.V. All rights reserved.


Yasue T.,Osaka Electro-Communication University | Suzuki M.,Osaka Electro-Communication University | Tsuno K.,Tokyo Electron | Goto S.,Tokyo Electron | And 2 more authors.
Review of Scientific Instruments | Year: 2014

Spin polarized electron beam is often used in material characterizations which relates to magnetism as well as in the high energy particle physics. The manipulation of the spin polarization toward the arbitrary direction is indispensable in such studies. In the present work, a novel multipole Wien filter is proposed as the three-dimensional spin manipulator, and a prototype 8-pole Wien filter is developed. It is applied to spin polarized low energy electron microscopy, and the variation of the magnetic contrast with managing the spin polarization is evaluated. It is confirmed that the novel multipole Wien filter can manipulate the spin polarization three-dimensionally. © 2014 AIP Publishing LLC.


Sannomiya T.,Tokyo Institute of Technology | Junesch J.,ETH Zurich | Hosokawa F.,JEOL Ltd. | Nagayama K.,National Institute of Physiological Science | And 2 more authors.
Ultramicroscopy | Year: 2014

A new fabrication method of carbon based phase plates for phase-contrast transmission electron microscopy is presented. This method utilizes colloidal masks to produce pores as well as disks on thin carbon membranes for phase modulation. Since no serial process is involved, carbon phase plate membranes containing hundreds of pores can be mass-produced on a large scale, which allows "disposal" of contaminated or degraded phase modulating objects after use. Due to the spherical shape of the mask colloid particles, the produced pores are perfectly circular. The pore size and distribution can be easily tuned by the mask colloid size and deposition condition. By using the stencil method, disk type phase plates can also be fabricated on a pore type phase plate. Both pore and disk type phase plates were tested by measuring amorphous samples and confirmed to convert the sinus phase contrast transfer function to the cosine shape. © 2014 Elsevier B.V.


Dahlin A.B.,Biosensors | Dahlin A.B.,Institute of Biomedical Engineering | Sannomiya T.,Biosensors | Sannomiya T.,Terabase Inc. | And 6 more authors.
Nano Letters | Year: 2011

We show how gold recrystallizes when under the influence of electrochemical potentials. This "cold annealing" occurs without charge transfer reactions and preserves nanoscale structural features. By performing the process on plasmonic nanostructures, grain growth is monitored noninvasively by optical spectroscopy. In this way, the influence from crystal structure on plasmon resonances can be investigated independently. Observed spectral changes are in excellent agreement with analytical models and changes in electron relaxation time and plasma frequency are calculated. © 2011 American Chemical Society.


PubMed | Terabase Inc., JEOL Ltd., ETH Zurich, Tokyo Institute of Technology and National Institute of Physiological Science
Type: | Journal: Ultramicroscopy | Year: 2014

A new fabrication method of carbon based phase plates for phase-contrast transmission electron microscopy is presented. This method utilizes colloidal masks to produce pores as well as disks on thin carbon membranes for phase modulation. Since no serial process is involved, carbon phase plate membranes containing hundreds of pores can be mass-produced on a large scale, which allows disposal of contaminated or degraded phase modulating objects after use. Due to the spherical shape of the mask colloid particles, the produced pores are perfectly circular. The pore size and distribution can be easily tuned by the mask colloid size and deposition condition. By using the stencil method, disk type phase plates can also be fabricated on a pore type phase plate. Both pore and disk type phase plates were tested by measuring amorphous samples and confirmed to convert the sinus phase contrast transfer function to the cosine shape.


Inayoshi Y.,Tokyo University of Agriculture and Technology | Minoda H.,Tokyo University of Agriculture and Technology | Minoda H.,Japan Science and Technology Agency | Arai Y.,Tera Base Co. | And 2 more authors.
Micron | Year: 2012

We have been developing a combination method for environmental TEM (E-TEM) and phase-plate TEM (P-TEM) that enables direct observations of the structure of biological molecules in aqueous solution. It is clearly demonstrated that the biological molecules in a water layer can be imaged by the combined method without any stain. The spatial resolution obtained in the present study was about 10. nm. This should be improved by using energy filtering. The image contrast of the specimen in water was reduced in comparison with that in vacuum. A model calculation that includes the effects of beam broadening, intensity decrease, and background increase caused by scattering from the water layer around the specimen shows that an increase in the thickness of the water layer reduces the contrast, intensity, and resolution of the image. © 2012 Elsevier Ltd.


Nishikawa K.,University of Tokyo | Kataoka M.,Institutes for Natural science | Nagata R.,Institutes for Natural science | Kitayama A.,Terabase Inc. | And 3 more authors.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 | Year: 2010

This paper reports a novel method to chemically modify specific nucleotides in a stretched double-stranded DNA, and to label the modified nucleotides with fluoresceinated antibodies in order to visualize them using both fluorescence microscope and AFM. We show that the modification is possible even with 48.5 kb DNA stretched to the full length and immobilized on a solid surface, where the bases are forming closed pairs and the interaction with the surface can cause steric hindrance to chemical reactions. This method is expected to be used not only for a high-resolution microscope-based direct DNA sequencing, but also for a method to align molecules and nano-particles using DNA as a template.


Kimoto T.,Japan National Institute of Materials Science | Arai Y.,Terabase Inc. | Ren X.,Japan National Institute of Materials Science
Applied Surface Science | Year: 2013

Cs3Sb photocathodes were fabricated at 8-16 C with sandwiched layers of Sb, Cs, and Sb deposited onto the fine tips of three cathodes at 15 C. After examining the influence of the cathode tip temperature on the changes in the quantum efficiencies of the Cs3Sb photocathodes during and after additional Cs depositions, we performed lifetime tests of the three Cs 3Sb photocathodes using a 405-nm semiconductor laser and 488-nm Ar ion laser. The decrease in the photocurrent with time was more rapid with the 488-nm laser irradiation than with the 405-nm laser irradiation, and continuous laser irradiation caused a much more rapid decrease in photoelectrons with time than intermittent laser irradiation did. We deposited a 0.32-0.64-nm-thick W film or a 0.43-nm-thick Cr film onto the Cs3Sb photocathode during the lifetime test at 0.9-1.0 × 10-7 Pa. We found that the passive WO3 or Cr2O3 film, which was formed at a reduced vacuum level of 1.6 × 10-7 Pa or during the continuous 405-nm laser irradiation, increased the lifetime of the Cs 3Sb photocathode by effectively protecting its surface against oxidation and evaporation of Cs. This protection effect was most effective at approximately 90 C. © 2013 Elsevier B.V. All rights reserved.

Loading Terabase Inc. collaborators
Loading Terabase Inc. collaborators