Hsu C.-Y.,Ten Chan General Hospital |
Wu C.-M.,Ten Chan General Hospital |
Cheng K.-L.,Ten Chan General Hospital
Journal of Rehabilitation Medicine | Year: 2014
Objective: We report here a rare case of anterior superior iliac spine avulsion fracture that presented initially as meralgia paraesthetica. Case report: A 14-year-old male sprinter presented with anterior superior iliac spine avulsion fracture, which was not observed on initial plain radiograph of the hip, but was diagnosed by ultrasound. Both clinical presentations and electrophysiological studies indicated meralgia paraesthetica. The lateral femoral cutaneous nerve of the thigh was probably compressed by an inguinal haematoma resulting from sartorius muscle strain, which was detected on musculoskeletal ultrasound. Computed tomography of the pelvis confirmed anterior superior iliac spine avulsion fracture. Conclusion: Meralgia paraesthetica in adolescents can be due to anterior superior iliac spine avulsion fracture. Sonography is a valuable tool for screening for muscular haematoma and occult fractures, which may allow clinicians to diagnose the nature of the muscle injury, and thus guide the most appropriate therapeutic strategy.Journal Compilation © 2014 Foundation of Rehabilitation Information.
PubMed | Ten Chan General Hospital and National Taiwan University Hospital
Type: Journal Article | Journal: Virchows Archiv : an international journal of pathology | Year: 2016
Benign, premalignant, and malignant endocervical glandular lesions occasionally show a gastric phenotype. We report 2 cases of gastric-type adenocarcinoma in situ (AIS) of the endocervix, not associated with lobular endocervical glandular hyperplasia or gastric-type adenocarcinoma. Cytologically, both showed endocervical glands with slightly enlarged nuclei, distinctive nucleoli, pseudostratified strips, and intracytoplasmic golden yellow mucin. Histologically, both lesions were situated in preexisting endocervical glands and presented columnar cells with voluminous pale eosinophilic cytoplasm and evident nuclear atypia. In case 1, the lesion was located at the mid-zone of the endocervical canal and, in case 2, at the outer endocervical canal with extension to the transformation zone and prominent intestinal metaplasia. In both, the cells showed voluminous cytoplasm containing gastric-type mucin, stained red by combined alcian blue/periodic acid-Schiff stain. Immunohistochemically, both lesions were positive for HIK1083 and p53, while negative for p16 and ER. Human papilloma virus (HPV) DNA was not detected by polymerase chain reaction. Our cases illustrate that gastric-type AIS can occur without lobular endocervical glandular hyperplasia. The lesions can occur in the outer cervical canal and present extensive intestinal differentiation. Awareness of this rare type of endocervical glandular lesion is important since they are pathogenetically different from the more common HPV-associated lesions and may become more prevalent in the HPV-eradicating era.
Tsai D.-C.,Ten Chan General Hospital |
Liu M.-C.,Kaohsiung Medical University |
Lin Y.-R.,Fooyin University |
Huang M.-F.,Kaohsiung Medical University |
And 2 more authors.
Food Chemistry | Year: 2016
Garlic (Allium sativum) is a long-cultivated plant that is widely utilized in cooking and has been employed as a medicine for over 4000 years. In this study, we fabricated standards and internal standards (ISs) for absolute quantification via reductive amination with isotopic formaldehydes. Garlic has four abundant organosulfur compounds (OSCs): S-allylcysteine, S-allylcysteinine sulfoxide, S-methylcysteine, and S-ethylcysteine are abundant in garlic. OSCs with primary amine groups were reacted with isotopic formaldehydes to synthesize ISs and standards. Cooked and uncooked garlic samples were compared, and we utilized tandem mass spectrometry equipped with a selective reaction monitoring technique to absolutely quantify the four organosulfur compounds. © 2015 Elsevier B.V. All rights reserved.
PubMed | China Medical University at Taichung, Kaohsiung Medical University, Ten Chan General Hospital and Kaohsiung Municipal Ta Tung Hospital
Type: Journal Article | Journal: Andrology | Year: 2015
Testosterone has been found to play important roles in mens sexual function. However, the effects of testosterone can be modulated by androgen receptor (AR) CAG repeat polymorphism. It could also contribute to the risk of erectile dysfunction (ED). The aim of this study is to evaluate the interaction of serum testosterone levels and AR CAG repeat polymorphism on the risk of ED in aging Taiwanese men. This cross-sectional data of Taiwanese men older than 40 years were collected from a free health screening held between August 2010 and August 2011 in Kaohsiung city, Taiwan. All participants completed a health questionnaires included five-item version of the International Index of Erectile Function (IIEF-5) and the International Prostate Symptoms Score, received a detailed physical examination and provided 20 cm3 whole blood samples for biochemical and genetic evaluation. The IIEF-5 was used to evaluate ED. Serum albumin, total testosterone (TT), and sex hormone-binding globulin levels were measured. Free testosterone level was calculated. AR gene CAG repeat polymorphism was determined by direct sequencing. Finally, 478 men with the mean age of 55.7 4.8 years were included. When TT levels were above 330 ng/dL, the effect of testosterone level on erectile function seemed to reach a plateau and a significantly negative correlation between AR CAG repeat length and the score of IIEF-5 was found (r = -0.119, p = 0.034). After adjusting for other covariates, the longer AR CAG repeat length was still an independent risk factor for ED in subjects with TT above 330 ng/dL (p = 0.006), but not in TT of 330 ng/dL or below. In conclusion, both serum testosterone levels and AR CAG repeat polymorphism can influence erectile function concomitantly. In subjects with normal TT concentration, those with longer AR CAG repeat lengths have a higher risk of developing ED.
PubMed | Kaohsiung Medical University, Ten Chan General Hospital, Government College University Lahore, Rashid Latif Medical College and National Sun Yat - sen University
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2014
Many red algae-derived natural products are known to have anticancer effects. The biological functions of the red alga Solieria robusta from the Karachi coast (Pakistan) remain unclear. Here, we prepared a methanolic extracts of S. robusta (MESR) to examine its possible anti-oral cancer effects and the corresponding mechanism of action. Cell viability of MESR-incubated oral cancer Ca9-22 cells was dose-responsively decreased (p<0.001). According to a propidium iodide (PI)-based assay the cell cycle distribution was dramatically changed, especially for subG1 accumulation. Annexin V/PI assay of apoptosis using flow cytometry also showed that MESR-incubated Ca9-22 cells were dose-responsively increased (p<0.001). For evaluation of oxidative stress in MESR-incubated Ca9-22 cells, we found that reactive oxygen species (ROS) were overexpressed dose- and time-responsively and mitochondrial depolarization was also increased (p<0.001). Taken together, MESR showed inhibitory effects on oral cancer proliferation coupled with apoptosis and oxidative stress.
PubMed | Ten Chan General Hospital, Chi Mei Medical Center, National Chung Hsing University, National Sun Yat - sen University and 3 more.
Type: | Journal: Chinese medicine | Year: 2016
The 2,2-diphenyl-1-picrylhydrazyl assay was used to determine free radical scavenging capability. Assessment of intracellular reactive oxygen species (ROS) was evaluated using oxidized 2,7-dichlorofluorescin diacetate and dihydroethidium staining. Trypan blue exclusion, colony formation, and anchorage-independent growth assays were used to determine cellular proliferation. Annexin V staining assay was used to assess cellular apoptosis by flow cytometry. Wound healing and Boydens well assays were used to detect the migration and invasion of cells. Gelatin zymography was used to detect matrix metalloproteinase (MMP-2 and MMP-9) activity. Western blotting was used to detect expression levels of various signaling pathway proteins.DPPH assay results indicated that Various concentrations (0.06-0.6mM) of
PubMed | Fooyin University, Ten Chan General Hospital, Kaohsiung Medical University and National Sun Yat - sen University
Type: Journal Article | Journal: Food chemistry | Year: 2015
Garlic (Allium sativum) is a long-cultivated plant that is widely utilized in cooking and has been employed as a medicine for over 4000 years. In this study, we fabricated standards and internal standards (ISs) for absolute quantification via reductive amination with isotopic formaldehydes. Garlic has four abundant organosulfur compounds (OSCs): S-allylcysteine, S-allylcysteinine sulfoxide, S-methylcysteine, and S-ethylcysteine are abundant in garlic. OSCs with primary amine groups were reacted with isotopic formaldehydes to synthesize ISs and standards. Cooked and uncooked garlic samples were compared, and we utilized tandem mass spectrometry equipped with a selective reaction monitoring technique to absolutely quantify the four organosulfur compounds.
Lin S.-W.,National Taiwan University |
Lin S.-W.,Ten Chan General Hospital |
Wang J.-L.,National Taiwan University
Biomedical Engineering - Applications, Basis and Communications | Year: 2010
High failure rates are expected in the treatment of osteoporotic proximal femoral fractures. A newly designed femoral neck locking dynamic hip screw has been developed to increase the fixation strength; a biomechanical examination was performed to prove its efficacy. Femoral surrogate specimens were used for the test. Unstable intertrochanteric fractures were created on specimens. Then these specimens were divided into two groups to test two different methods of fixation: (1) the conventional dynamic hip screw and (2) the new designed femoral neck fixation dynamic hip screw. For each specimen, the peak cyclic loading was 800 N, and a total of 20,000 cycles were applied. The vertical displacement, rotation around the long axis of lag screw, and the varus rotation around the center of femoral head between the two groups were compared at specific intervals. All the conventional dynamic hip screw constructs failed within 1000 cycles, while no gross mechanical failure was recorded for the femoral neck fixation dynamic hip construct even after 20,000 loading cycles. The mechanical test comparing the two constructs at the end of each 1000 loading cycles demonstrated that the femoral neck fixation dynamic hip screw construct is stronger than that of the conventional dynamic hip screw. For the fixation of proximal femoral fractures, the femoral neck fixation dynamic hip screw can provide a stronger support than what a conventional dynamic hip screw can do. At the same time, femoral neck can be a suitable site for the application of proximal locking screws. © 2010 National Taiwan University.
Lin S.-W.,National Taiwan University |
Lin S.-W.,Ten Chan General Hospital |
Chiang C.-K.,National Taiwan University |
Yang C.-L.,National Taiwan University |
Wang J.-L.,National Taiwan University
Spine | Year: 2010
Study Design.: An in vivo clinical trial, and an in vitro cadaveric biomechanical and micromorphologic analysis. Objective.: To find the feasibility of using viscoplastic bone cement for vertebroplasty. Summary of Background Data.: Vertebroplasty involved in bone cement reinforcement of fractured vertebra has shown promising clinical results. The most frequently observed complication of vertebroplasty is the cement leakage during surgery. Many methods were proposed and were successful at reducing the risk of leakage, such as creating a void within vertebra to reduce the injection pressure, increasing the cement viscosity to reduce the cement infiltration, etc. Nevertheless, a more cost-effective and safer surgery method is still the goal for many spine surgeons and researchers. Methods.: To deliver the viscoplastic bone cement into the vertebra, a unipedicular tract and a void in the vertebra was created using a curette. The viscoplastic bone cement was then delivered into the void piece by piece and tamped for compactness with a blunt end tool. For the in vitro biomechanical test, 7 thoracic vertebrae were used. The intact specimens were compressed to lose 25% of its intact height, and then augmented with viscoplastic bone cement. Postaugmentation CT scanning was taken to examine the cement distribution, leakage path, and cement filling ratio within the vertebra. Postaugmentation compression test was conducted to examine the vertebral strength and stiffness, and then compared with the intact ones. Finally, the vertebrae were cut into slices for micromorphologic analysis. Results.: The 6 in vivo clinical trials were all successfully operated with significant pain relief and showed no leakage during and after the surgery. The in vitro biomechanical test showed the cement augmentation significantly increased the vertebral strength (pre 3164 (229) N vs. post 3905 (484) N, P < 0.003), but tentatively decreased the vertebral stiffness (pre 1074 (74) N/mm vs. post 801 (370) N/mm, P = 0.081). The postaugmentation CT scanning showed the cement was well confined within the vertebra and the cement filling ratio was 21% (ranged from 15% to 29%). The depth that the viscoplastic bone cement infiltrated into the cancellous bone was 3.5 (0.6) mm, which is less than the depth [8.3 (2.2) mm, P < 0.001] of standard viscous bone cement vertebroplasty. Conclusion.: Vertebroplasty using viscoplastic bone cement is clinically feasible and can effectively improve the vertebral strength and reduce the cement infiltration depth. The risk of cement leakage can also be decreased by using viscoplastic bone cement. © 2010, Lippincott Williams & Wilkins.
Chiang Y.-P.,Mackay Memorial Hospital |
Hsu C.-Y.,Mackay Memorial Hospital |
Hsu C.-Y.,Ten Chan General Hospital |
Lien W.-C.,Mackay Memorial Hospital |
Chang Y.-J.,Mackay Memorial Hospital
Journal of Clinical Ultrasound | Year: 2014
Background: To describe the sonographic (US) features of pathologically confirmed subungual glomus tumors. Methods: We retrospectively analyzed cases of pathologically confirmed subungual glomus tumors in patients presenting between December 1, 2008 and October 31, 2012, selected from cases in the musculoskeletal US database of a single institution. Results: Data on 14 patients with pathologically proven glomus tumors were retrieved from the US database. The tumor size ranged from 1.9 to 10.0 mm (mean±SD, 5.3±2.3 mm). Well-circumscribed tumors with clear margins were identified on US in 12 cases (86%): 11 (92%) tumors were hypoechoic and 1 (8%) was isoechoic. On power or color Doppler US, 11 of these 12 tumors (92%) appeared hypervascular. Two of the 14 tumors did not show a clearly circumscribed mass on gray-scale US, and bony erosion was the only US finding in one of the two cases. Seven of the 14 patients (50%) showed focal bony erosion in the underlying phalangeal bone. Conclusions: A hypervascular mass in the nail bed was the most common US finding associated with subungual glomus tumors. We found that focal hypervascularity supports the diagnosis, and bony erosion is commonly associated with digital glomus tumors. © 2014 Wiley Periodicals, Inc.