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Khursheed S.,CSK Himachal Pradesh Agricultural University | Raj D.,AIR INDIA | Ganie N.A.,Temperate Sericulture Research Institute
Journal of Applied Horticulture | Year: 2013

The population dynamics of Aulacophora foveicollis Lucas in relation to abiotic factors was studied on cucumber (Cucumis sativus), var. 'Khira-90' during 2009 and 2010. Incidence of red pumpkin beetle in field indicated that its initial activity and peak period varied with the locations and prevailing weather conditions. At Palampur, the insect was found active from second fortnight of April with three peaks during 2nd and 4th weeks of May and 3rd week of July, 2009 whereas, one major peak during 2nd week of May was recorded in 2010. At farmer's field, Bara (Hamirpur) the insect first appearance was noticed during first fortnight of March and reached to its peak during 3rd and 2nd weeks of April, 2009 and 2010, respectively. The highest plant infestation (100 %) was observed when the crop was at its early growing stage. The correlation studies revealed that average minimum temperature showed significant negative correlation at farmer's field whereas other weather parameters had no significant effect on the beetle population at Palampur as well as Bara.


Sabina A.,American University in the Emirates | Shafiq W.A.,Direct Of Research | Ashraf B.M.,Center for Plant Biotechnology | Shabir W.,Temperate Sericulture Research Institute | Nawsheeba W.,Center for Plant Biotechnology
Research Journal of Biotechnology | Year: 2015

Rice yields have now apparently reached a plateau possibly due to use of common dwarfing gene sources and alleles contributing yield potential that has led to narrowing genetic base of these high yielding cultivars. Therefore, the use of local germplasm calls for its evaluation and characterization before embarking on their use in a systematic breeding programme. In the present study 40 SSR primers generated a total of 250 alleles across these genotypes with a range of 2-11 alleles per locus and a mean of 6.89 alleles per locus. The PIC values, measuring allelic diversity and polymorphism ranged from 0.490 (RM2592) on chromosome-1 to 0.806 (RM585) on the chromosome-6, with a mean PIC value of 0.719 per locus. Similarity coefficients (measuring relatedness) were used to construct the dendogram and clustering pattern. Pairwise similarity coefficients ranged from 0.10 to 0.89, with the average similarity coefficient around 0.40. Similarity coefficient value revealed presence of good genetic diversity among these genotypes at the molecular level. Clustering pattern revealed grouping of these genotypes into 5 clusters. All the landraces got grouped into 2 clusters viz. cluster-I (13) and cluster-II (7) except Rehman Batt-1 which got grouped in the cluster-III which mostly contained genotypes for higher altitude conditions. High yielding varieties for valley basin conditions formed cluster-V (5 cultivars), while cluster-IV contained cutivars mostly of K-series.


Wani Shabir A.,Temperate Sericulture Research Institute | Ashraf B.M.,Center for Plant Biotechnology | Kamili A.S.,Extension Education | Wani N.,Center for Plant Biotechnology | And 2 more authors.
Research Journal of Biotechnology | Year: 2014

India is recognized as one of the twelve mega biodiversity centers of the world and harbors 11.9% of the world flora. The plant genetic diversity is being recognized by the International Conservation Community as a basic key component for the sustainable development of agriculture, horticulture and sericulture. Rich source of wild relatives of mulberry flora are reported to occur in Himalayan belts. Germplasm evaluation is essential in any crop improvement programme and genetic characterization at morphological and molecular level is very vital for breeding programs to be successful. Thirty RAPD markers generated a total of 179 alleles across 17 genotypes and the number of alleles per locus varied from 4 (OPA 13) to 9 (OPBB 13) with an average of 5.96 per locus. The similarity coefficient ranged from 0.74 to 0.93 with an average of 0.84. The highest similarity value 0.93 was recorded between SKM- 33 and KNG and SKM -33 and Goshoerami, lowest similarity coefficient (0.74) was found between local mulberry and Kokuso- 20. Cluster analysis by Unweighted Pair Group Method using Arithmetic average (UPGMA) grouped 17 mulberry genotypes/selections into 8 different clusters. Cluster-2 contain highest (6) genotypes.

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