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Patel M.,National Institute of Pharmaceutical Education and Research | Antala B.,National Institute of Pharmaceutical Education and Research | Shrivastava N.,National Institute of Pharmaceutical Education and Research | Shrivastava N.,tel Pharmaceutical Education And Research Development Perd Center
Critical Reviews in Eukaryotic Gene Expression | Year: 2015

Cell competition is a type of short-range cell–cell interaction first observed in Drosophila melanogaster. In two heterogeneous cell populations, cells that have a higher fitness level would have a competitive advantage and grow at the cost of neighbor cells that have comparatively lower fitness. This interaction is due to differences in expression levels of a specific protein in the two cell populations, and it is known as cell competition. In this review, we have studied recent findings of cell competition in different biological processes in Drosophila as well as mammalian systems. The purpose of this review is to collate important studies of competitive cell interactions, and to understand its roles and importance as a central phenomenon. This review provides evidence of the relevance of cell competition in various physiological and pathological conditions, such as size control in organ development, stem cell maintenance, tissue repair, organ regeneration, aging, formation of memory, and cancer. © 2015 Begell House, Inc. Source


Yagnik B.,tel Pharmaceutical Education And Research Development Perd Center | Padh H.,Sardar Patel University | Desai P.,tel Pharmaceutical Education And Research Development Perd Center
Microbes and Infection | Year: 2016

Use of food grade Lactococcus lactis (L. lactis) is fast emerging as a safe alternative for delivery of DNA vaccine. To attain efficient DNA delivery, L. lactis, a non-invasive bacterium is converted to invasive strain either by expressing proteins like Internalin A (InlA) or Fibronectin binding protein A (FnBPA) or through chemical treatments. However the safety status of invasive L. lactis is questionable. In the present report, we have shown that non-invasive L. lactis efficiently delivered the newly constructed reporter plasmid pPERDBY to mammalian cells without any chemical enhancers. The salient features of the vector are; I) Ability to replicate in two different hosts; Escherichia coli (E. coli) and Lactic Acid Bacteria (LAB), II) One of the smallest reporter plasmid for DNA vaccine, III) Enhanced Green Fluorescence Protein (EGFP) linked to Multiple Cloning Site (MCS), IV) Immunostimulatory CpG motifs functioning as an adjuvant. Expression of EGFP in pPERDBY transfected CHO-K1 and Caco-2 cells demonstrates its functionality. Non-invasive r-L. lactis was found efficient in delivering pPERDBY to Caco-2 cells. The in vitro data presented in this article supports the hypothesis that in the absence of invasive proteins or relevant chemical treatment, L. lactis was found efficient in delivering DNA to mammalian cells. © 2015 Institut Pasteur. Source


Yadav H.,National Institute of Pharmaceutical Education and Research | Mungara P.,National Institute of Pharmaceutical Education and Research | Jivrajani M.,tel Pharmaceutical Education And Research Development Perd Center | Nivsarkar M.,tel Pharmaceutical Education And Research Development Perd Center | Anandjiwala S.,National Institute of Pharmaceutical Education and Research
Journal of Liquid Chromatography and Related Technologies | Year: 2012

Vitex negundo Linn (Verberaceae) is an important drug in Indian, Chinese, and Malaysian Systems of medicine. The leaves are reported to show anti-inflammatory, anti-ulcer, anti-implantation, and analgesic activities. They contain negundoside, nishindaside, agnuside, luteolin, vitexicarpin, ursolic acid, and β-sitosterol. Lupeol, an important marker compound has not been reported from V. negundo until recently. Our preliminary TLC experiments indicated the presence of lupeol in the leaves of V. negundo. Subsequently, lupeol was isolated by preparative TLC. The identity of lupeol was confirmed by comparing the mass spectra [m/z value 427.8 (M+1) and 425.8 (M 1)]; melting point (215C, using DSC) and co-TLC with the standard compound. A High Performance Thin Layer Chromatography (HPTLC) method for the quantification of five marker compounds, namely, negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol from the leaves of V. negundo was developed and validated as per ICH guidelines. The amounts of negundoside, ursolic acid, eugenol, lupeol, and β-sitosterol were found to be 1.048, 0.013, 0.115, 0.116, and 0.043% w/w, respectively. The developed methods were found to be accurate, precise, and reproducible. They can be used for routine quality control of herbal material and formulations containing these compounds. © 2012 Copyright Taylor and Francis Group, LLC. Source


Jivrajani M.,tel Pharmaceutical Education And Research Development Perd Center | Shrivastava N.,tel Pharmaceutical Education And Research Development Perd Center | Nivsarkar M.,tel Pharmaceutical Education And Research Development Perd Center
Journal of Microbiological Methods | Year: 2013

A method for bacterial minicell purification was developed by combining antibiotic (ceftriaxone) lysis and filtration. This method is fast, cost effective and facilitates high yield of purified minicells, with no parent strain contamination as confirmed by fluorescent microscopy, average particle size and polydispersity index. © 2012 Elsevier B.V.. Source


Agarwal M.,tel Pharmaceutical Education And Research Development Perd Center | Shrivastava N.,tel Pharmaceutical Education And Research Development Perd Center | Padh H.,tel Pharmaceutical Education And Research Development Perd Center
Plant Breeding | Year: 2011

In most economically important dioecious plants such as Simmondsia chinensis (Jojoba), sex identification based on flowering can only be made after 1-5years of plantation. Identification of sex-associated molecular markers for ascertaining sex at seedling stage is a desirable prerequisite for optimal plantation and economical benefits. Jojoba is being developed as a plantation crop in the arid regions of Western India due to the commercial benefits of the unique waxy content of its seeds. In the present study, sex-linked DNA markers have been identified in S. chinensis using the AFLP technique. From sixteen primers combinations used for AFLP analysis, two combinations EcoRI-TAC/MseI-GCG and EcoRI-GC/MseI-GCG resulted in amplification of sex-linked markers. Two male-specific markers of ~525bp and ~325bp were identified using the primer combinations EcoRI-GC/MseI-GCG and EcoRI-TAC/MseI-GCG, respectively. A female-specific marker of ~270bp was also identified with the primer combination EcoRI-TAC/MseI-GCG. In future, SCAR markers developed from these sex-specific markers will provide an effective and reliable method for sex identification. © 2010 Blackwell Verlag GmbH. Source

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