Hagihara A.,Kyushu University |
Miyazaki S.,Kyushu University |
Miyazaki S.,Teikyo Heisei University |
Abe T.,Kyushu University
European Archives of Psychiatry and Clinical Neuroscience | Year: 2012
Although several case reports have suggested a relationship between accessing Internet suicide sites and the incidence of suicide, the influence of the Internet on the incidence of suicide is not known. Thus, we examined the association between Internet suicide-related searches and the incidence of suicide in 20-and 30-year-old individuals in Japan. The Box-Jenkins transfer function model was applied to monthly time series data from January 2004 to May 2010 (77 months). The terms ''hydrogen sulfide, '' ''hydrogen sulfide suicide, '' and ''suicide hydrogen sulfide suicide'' at (t-11) were related to the incidence of suicide among people aged in their 20 s (P = 0.005, 0.005, and 0.006, respectively) and people aged in their 30 s (P = 0.013, 0.011, and 0.012, respectively). ''BBS on suicide'' at (t-5) and ''suicide by jumping'' at (t-6) were related to the incidence of suicide in people aged 30-39 (P = 0.006 and 0.001, respectively). Internet searches for specific suicide-related terms are related to the incidence of suicide among 20-and 30-year-old individuals in Japan. Routine interrogation by a clinician about visiting Internet suicide websites and stricter regulation of these websites may reduce the incidence of suicide among young people. © 2011 Springer-Verlag.
Yamaguchi Y.,Teikyo Heisei University |
Tanikawa K.,Japan National Astronomical Observatory
Progress of Theoretical Physics | Year: 2011
A certain region in the phase space for the area- and orientation- preserving Hénon map is filled with the resonance chains. Taking this fact into account, the concept of block word has been introduced in the preceding paper. The concept of resonance chains in the symbol plane is also introduced and the marquetry structure of the symbol plane is investigated. If the orbit in the horseshoe travels over the resonance chains, the orbit is represented by a block word sequence. There are four purposes discussed in this paper. The first purpose is to introduce a, new concept of parity to characterize the periodic orbits. The second is to study the detailed structure of the symbol plane. The third is to clarify the behaviors of symmetric periodic orbits in the resonance chain and determine their block word sequences. The fourth is to propose an improved construction method to make a braid for a, non-Birkhoff periodic orbit in a resonance chain.
Kodama H.,Teikyo University |
Kodama H.,Teikyo Heisei University |
Fujisawa C.,Teikyo University |
Bhadhprasit W.,Teikyo University
Current Drug Metabolism | Year: 2012
Copper is an essential trace element required by all living organisms. Excess amounts of copper, however, results in cellular damage. Disruptions to normal copper homeostasis are hallmarks of three genetic disorders: Menkes disease, occipital horn syndrome, and Wilson's disease. Menkes disease and occipital horn syndrome are characterized by copper deficiency. Typical features of Menkes disease result from low copper-dependent enzyme activity. Standard treatment involves parenteral administration of copper-histidine. If treatment is initiated before 2 months of age, neurodegeneration can be prevented, while delayed treatment is utterly ineffective. Thus, neonatal mass screening should be implemented. Meanwhile, connective tissue disorders cannot be improved by copper-histidine treatment. Combination therapy with copper-histidine injections and oral administration of disulfiram is being investigated. Occipital horn syndrome characterized by connective tissue abnormalities is the mildest form of Menkes disease. Treatment has not been conducted for this syndrome. Wilson's disease is characterized by copper toxicity that typically affects the hepatic and nervous systems severely. Various other symptoms are observed as well, yet its early diagnosis is sometimes difficult. Chelating agents and zinc are effective treatments, but are inefficient in most patients with fulminant hepatic failure. In addition, some patients with neurological Wilson's disease worsen or show poor response to chelating agents. Since early treatment is critical, a screening system for Wilson's disease should be implemented in infants. Patients with Wilson's disease may be at risk of developing hepatocellular carcinoma. Understanding the link between Wilson's disease and hepatocellular carcinoma will be beneficial for disease treatment and prevention. © 2012 Bentham Science Publishers.
Obinata T.,Teikyo Heisei University |
Ono K.,Emory University |
Ono S.,Emory University
Journal of Cell Science | Year: 2010
The myoepithelial sheath of the Caenorhabditis elegans somatic gonad has non-striated actomyosin networks that provide contractile forces during ovulation, a process in which a mature oocyte is expelled from the ovary. Troponin T and troponin C are known regulators of contraction of the myoepithelial sheath. These are two of the three components of the troponin complex that is generally considered as a striated-muscle-specific regulator of actomyosin contraction. Here, we report identification of troponin I as the third component of the troponin complex that regulates ovulatory contraction of the myoepithelial sheath. C. elegans has four genes encoding troponin-I isoforms. We found that tni-1 and unc-27 (also known as tni-2) encode two major troponin-I isoforms in the myoepithelial sheath. Combination of RNA interference and mutation of tni-1 and unc-27 resulted in loss of the troponin-I protein in the gonad and caused sterility due to defective contraction of the myoepithelial sheath. Troponin-I-depleted gonads were hypercontracted, which is consistent with the function of troponin I as an inhibitor of actomyosin contraction. Troponin I was associated with nonstriated actin networks in a tropomyosin-dependent manner. Our results demonstrate that troponin I regulates contraction of nonstriated actomyosin networks and is an essential cytoskeletal component of the C. elegans reproductive system.
Hisano Y.,Osaka University |
Hisano Y.,RIKEN |
Kobayashi N.,Osaka University |
Kobayashi N.,Teikyo Heisei University |
And 2 more authors.
PLoS ONE | Year: 2012
Sphingosine-1-phosphate (S1P), a sphingolipid metabolite that is produced inside the cells, regulates a variety of physiological and pathological responses via S1P receptors (S1P1-5). Signal transduction between cells consists of three steps; the synthesis of signaling molecules, their export to the extracellular space and their recognition by receptors. An S1P concentration gradient is essential for the migration of various cell types that express S1P receptors, such as lymphocytes, pre-osteoclasts, cancer cells and endothelial cells. To maintain this concentration gradient, plasma S1P concentration must be at a higher level. However, little is known about the molecular mechanism by which S1P is supplied to extracellular environments such as blood plasma. Here, we show that SPNS2 functions as an S1P transporter in vascular endothelial cells but not in erythrocytes and platelets. Moreover, the plasma S1P concentration of SPNS2-deficient mice was reduced to approximately 60% of wild-type, and SPNS2-deficient mice were lymphopenic. Our results demonstrate that SPNS2 is the first physiological S1P transporter in mammals and is a key determinant of lymphocyte egress from the thymus. © 2012 Hisano et al.
Hagihara A.,Kyushu University |
Hasegawa M.,Teikyo Heisei University |
Abe T.,Kyushu University |
Nagata T.,Kyushu University |
And 2 more authors.
JAMA - Journal of the American Medical Association | Year: 2012
Context: Epinephrine is widely used in cardiopulmonary resuscitation for out-of-hospital cardiac arrest (OHCA). However, the effectiveness of epinephrine use before hospital arrival has not been established. Objective: To evaluate the association between epinephrine use before hospital arrival and short- and long-term mortality in patients with cardiac arrest. Design, Setting, and Participants: Prospective, nonrandomized, observational propensity analysis of data from 417 188 OHCAs occurring in 2005-2008 in Japan in which patients aged 18 years or older had anOHCAbefore arrival of emergency medical service (EMS) personnel, were treated by EMS personnel, and were transported to the hospital. Main Outcome Measures: Return of spontaneous circulation before hospital arrival, survival at 1 month after cardiac arrest, survival with good or moderate cerebral performance (Cerebral Performance Category [CPC] 1 or 2), and survival with no, mild, or moderate neurological disability (Overall Performance Category [OPC] 1 or 2). Results: Return of spontaneous circulation before hospital arrival was observed in 2786 of 15 030 patients (18.5%) in the epinephrine group and 23 042 of 402 158 patients (5.7%) in the no-epinephrine group (P < .001); it was observed in 2446 (18.3%) and 1400 (10.5%) of 13 401 propensity-matched patients, respectively (P < .001). In the total sample, the numbers of patients with 1-month survival and survival with CPC 1 or 2 and OPC 1 or 2, respectively, were 805 (5.4%), 205 (1.4%), and 211 (1.4%) with epinephrine and 18 906 (4.7%), 8903 (2.2%), and 8831 (2.2%) without epinephrine (all P < .001). Corresponding numbers in propensity-matched patients were 687 (5.1%), 173 (1.3%), and 178 (1.3%) with epinephrine and 944 (7.0%), 413 (3.1%), and 410 (3.1%) without epinephrine (all P < .001). In all patients, a positive association was observed between prehospital epinephrine and return of spontaneous circulation before hospital arrival (adjusted odds ratio [OR], 2.36; 95% CI, 2.22-2.50; P < .001). In propensity-matched patients, a positive association was also observed (adjusted OR, 2.51; 95% CI, 2.24-2.80; P < .001). In contrast, among all patients, negative associations were observed between prehospital epinephrine and long-term outcome measures (adjusted ORs: 1-month survival, 0.46 [95% CI, 0.42-0.51]; CPC 1-2, 0.31 [95% CI, 0.26-0.36]; and OPC 1-2, 0.32 [95% CI, 0.27-0.38]; all P < .001). Similar negative associations were observed among propensity-matched patients (adjusted ORs: 1-month survival, 0.54 [95% CI, 0.43-0.68]; CPC 1-2, 0.21 [95% CI, 0.10-0.44]; and OPC 1-2, 0.23 [95% CI, 0.11-0.45]; all P < .001). Conclusion: Among patients with OHCA in Japan, use of prehospital epinephrine was significantly associated with increased chance of return of spontaneous circulation before hospital arrival but decreased chance of survival and good functional outcomes 1 month after the event. ©2012 American Medical Association. All rights reserved.
Teikyo Heisei University | Date: 2014-12-24
An object of the present invention is to effectively induce cancer cell apoptosis using the anti-TRAIL-R1 antibody(ies) and the anti-TRAIL-R2 antibody(ies) and to reduce the toxicity imposed on normal cells. The present invention relates to recombinant obligate anaerobic Gram-positive bacteria that include a nucleic acid encoding a fusion protein having 3 or more anti-TRAIL-R1 single-chain antibodies and/or 3 or more anti-TRAIL-R2 single-chain antibodies, in an expressible state.
Hattori A.,Kyoto University |
Tsujimoto M.,Teikyo Heisei University
Journal of Biochemistry | Year: 2013
The human endoplasmic reticulum aminopeptidase (ERAP) 1 and 2 proteins were initially identified as homologues of human placental leucine aminopeptidase/insulin-regulated aminopeptidase. They are categorized as a unique class of proteases based on their subcellular localization on the luminal side of the endoplasmic reticulum. ERAPs play an important role in the N-terminal processing of the antigenic precursors that are presented on the major histocompatibility complex (MHC) class I molecules. ERAPs are also implicated in the regulation of a wide variety of physiological phenomena and pathogenic conditions. In this review, the current knowledge on ERAPs is summarized. © The Authors 2013. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
Teikyo Heisei University | Date: 2016-11-16
An object of the present invention is to effectively induce cancer cell apoptosis using the anti-TRAIL-R1 antibody(ies) and the anti-TRAIL-R2 antibody(ies) and to reduce the toxicity imposed on normal cells. The present invention relates to recombinant obligate anaerobic Gram-positive bacteria that comprise a nucleic acid encoding a fusion protein comprising 3 or more anti-TRAIL-R1 single-chain antibodies and/or 3 or more anti-TRAIL-R2 single-chain antibodies, in an expressible state.
Teikyo Heisei University | Date: 2015-05-20
An object of the present invention is to provide an antitumor agent and/or a marker for tumor detection with low invasiveness, few side effects, and high target specificity. Through the use of a recombinant obligate anaerobic gram-positive bacterium as an active ingredient containing a nucleic acid encoding a secretory fusion protein comprising a signal peptide, a low-molecular-weight single-chain antibody, and a functional peptide, an antitumor agent that enables delivery of a functional peptide to a target cell and/or a marker for tumor detection that enables monitoring of therapeutic effects over time are provided.