Takano N.,Teikyo Heisei University
IFMBE Proceedings | Year: 2017
Use of bandpass filter as a preprocessing for Hilbert transform in electrocardiographic QRS complex feature detection is discussed here, based on computer simulation results. The main topic is onset and offset estimation on QRS complex. The bandpass filter is configured for the frequency range of QRS complex. Hilbert transform is applied to the filter output in order to generate an envelope signal which most reflects the QRS complex. From such an envelope signal, the onset and offset of each QRS complex are estimated by means of the tangent line projection method. The simulation ECG waveforms were generated from a dipole source vector with the lead vectors of the standard limb leads as an application of the lead field theory and the single dipole source model. The simulation results demonstrated that the estimation was stable against changes in the ECG source-measurement relation, which depends on the ECG lead selection and the ECG lead vector. © Springer Nature Singapore Pte Ltd. 2018.
Omori S.,Teikyo Heisei University
IEEJ Transactions on Electronics, Information and Systems | Year: 2017
Mid-infrared lasers having the wavelength of the vicinity of 3μm are considered as effective for treatment of biomedical tissue because of the strong absorption to water. The drawback of 3μm laser is the difficulty to propagate the silica optical fiber. We have reported the optical fiber tip oscillation laser that has a Er, Cr: YAGG laser crystal and coupling lenses on the tip of a silica fiber outputs 2.8μm laser pumped by laser diode (LD). In this paper, we demonstrated a lens-less laser head for the optical fiber tip oscillation. By using the silica optical fiber having a core diameter of 105μm and a numerical aperture (NA) of 0.12, the average output power of 1.0W was achieved from the lens-less laser head during a squire wave modulation for suppressing heat generation. © 2017 The Institute of Electrical Engineers of Japan.
Nakamura T.J.,University of California at Los Angeles |
Nakamura T.J.,Teikyo Heisei University |
Nakamura W.,Osaka University |
Yamazaki S.,Vanderbilt University |
And 4 more authors.
Journal of Neuroscience | Year: 2011
Disruptions in sleep/wake cycles, including decreased amplitude of rhythmic behaviors and fragmentation of the sleep episodes, are commonly associated with aging in humans and other mammals. While there are undoubtedly many factors contributing to these changes, a body of literature is emerging, suggesting that an age-related decline in the central circadian clock in the suprachiasmatic nucleus (SCN) may be a key element responsible. Toexplore age-related changes in the SCN, wehave performed in vivo multiunit neural activity(MUA)recordings from the SCN of freely moving young (3-5 months) and middle-aged (13-18 months) mice. Importantly, the amplitude of day-night difference in MUA was significantly reduced in the older mice. Wealso found that the neural activity rhythms are clearly degraded in the sub paraventricular zone, one of the main neural outputs of the SCN. Surprisingly, parallel studies indicate that the molecular clockwork in the SCN as measured by PER2 exhibited only minor deficits at this same age. Thus, the circadian output measured at the level of neural activity rhythms in the SCN is degraded by aging, and this decline occurs before the disruption of key components of the molecular clockwork. © 2011 the authors.
Kawashima T.,Teikyo Heisei University |
Sato T.,University of Tokyo
Attention, Perception, and Psychophysics | Year: 2015
Numerosity judgments of simultaneous talkers were examined. Listeners were required to report the number of talkers heard when this number varied (1 to 13). Spatial location of talkers (1 or 6 locations), duration of talker voices (0.8 s, 5.0 s, and 15.0 s), and gender arrangement of talkers also were manipulated in four experiments. In all experiments, the proportion of correct numerosity judgments monotonically decreased as talker numbers increased. Perceptual limits, defined as talker numbers with proportion correct scores of 0.5, varied between 3 to 5 talkers, on average, depending on listening conditions, and were significantly higher for spatially separated talkers, for the longer voices, and for the mixed gender voices (Experiments 1, 2, and 3). In addition, Experiment 4 found that average numerosity response times increased monotonically over a range of one to four talkers. These results support the idea that, before counting talkers, listeners perceptually segregate talkers to render numerosity judgments. They also suggest that our functional auditory world for simultaneous voices may consist of, at most, three to five talkers depending on listening situations. In light of these results, possible causes for such perceptual limits are discussed. © 2015, The Psychonomic Society, Inc.
Obinata T.,Teikyo Heisei University |
Ono K.,Emory University |
Ono S.,Emory University
Journal of Cell Science | Year: 2010
The myoepithelial sheath of the Caenorhabditis elegans somatic gonad has non-striated actomyosin networks that provide contractile forces during ovulation, a process in which a mature oocyte is expelled from the ovary. Troponin T and troponin C are known regulators of contraction of the myoepithelial sheath. These are two of the three components of the troponin complex that is generally considered as a striated-muscle-specific regulator of actomyosin contraction. Here, we report identification of troponin I as the third component of the troponin complex that regulates ovulatory contraction of the myoepithelial sheath. C. elegans has four genes encoding troponin-I isoforms. We found that tni-1 and unc-27 (also known as tni-2) encode two major troponin-I isoforms in the myoepithelial sheath. Combination of RNA interference and mutation of tni-1 and unc-27 resulted in loss of the troponin-I protein in the gonad and caused sterility due to defective contraction of the myoepithelial sheath. Troponin-I-depleted gonads were hypercontracted, which is consistent with the function of troponin I as an inhibitor of actomyosin contraction. Troponin I was associated with nonstriated actin networks in a tropomyosin-dependent manner. Our results demonstrate that troponin I regulates contraction of nonstriated actomyosin networks and is an essential cytoskeletal component of the C. elegans reproductive system.
Hagihara A.,Kyushu University |
Hasegawa M.,Teikyo Heisei University |
Abe T.,Kyushu University |
Nagata T.,Kyushu University |
And 2 more authors.
JAMA - Journal of the American Medical Association | Year: 2012
Context: Epinephrine is widely used in cardiopulmonary resuscitation for out-of-hospital cardiac arrest (OHCA). However, the effectiveness of epinephrine use before hospital arrival has not been established. Objective: To evaluate the association between epinephrine use before hospital arrival and short- and long-term mortality in patients with cardiac arrest. Design, Setting, and Participants: Prospective, nonrandomized, observational propensity analysis of data from 417 188 OHCAs occurring in 2005-2008 in Japan in which patients aged 18 years or older had anOHCAbefore arrival of emergency medical service (EMS) personnel, were treated by EMS personnel, and were transported to the hospital. Main Outcome Measures: Return of spontaneous circulation before hospital arrival, survival at 1 month after cardiac arrest, survival with good or moderate cerebral performance (Cerebral Performance Category [CPC] 1 or 2), and survival with no, mild, or moderate neurological disability (Overall Performance Category [OPC] 1 or 2). Results: Return of spontaneous circulation before hospital arrival was observed in 2786 of 15 030 patients (18.5%) in the epinephrine group and 23 042 of 402 158 patients (5.7%) in the no-epinephrine group (P < .001); it was observed in 2446 (18.3%) and 1400 (10.5%) of 13 401 propensity-matched patients, respectively (P < .001). In the total sample, the numbers of patients with 1-month survival and survival with CPC 1 or 2 and OPC 1 or 2, respectively, were 805 (5.4%), 205 (1.4%), and 211 (1.4%) with epinephrine and 18 906 (4.7%), 8903 (2.2%), and 8831 (2.2%) without epinephrine (all P < .001). Corresponding numbers in propensity-matched patients were 687 (5.1%), 173 (1.3%), and 178 (1.3%) with epinephrine and 944 (7.0%), 413 (3.1%), and 410 (3.1%) without epinephrine (all P < .001). In all patients, a positive association was observed between prehospital epinephrine and return of spontaneous circulation before hospital arrival (adjusted odds ratio [OR], 2.36; 95% CI, 2.22-2.50; P < .001). In propensity-matched patients, a positive association was also observed (adjusted OR, 2.51; 95% CI, 2.24-2.80; P < .001). In contrast, among all patients, negative associations were observed between prehospital epinephrine and long-term outcome measures (adjusted ORs: 1-month survival, 0.46 [95% CI, 0.42-0.51]; CPC 1-2, 0.31 [95% CI, 0.26-0.36]; and OPC 1-2, 0.32 [95% CI, 0.27-0.38]; all P < .001). Similar negative associations were observed among propensity-matched patients (adjusted ORs: 1-month survival, 0.54 [95% CI, 0.43-0.68]; CPC 1-2, 0.21 [95% CI, 0.10-0.44]; and OPC 1-2, 0.23 [95% CI, 0.11-0.45]; all P < .001). Conclusion: Among patients with OHCA in Japan, use of prehospital epinephrine was significantly associated with increased chance of return of spontaneous circulation before hospital arrival but decreased chance of survival and good functional outcomes 1 month after the event. ©2012 American Medical Association. All rights reserved.
Teikyo Heisei University | Date: 2014-12-24
An object of the present invention is to effectively induce cancer cell apoptosis using the anti-TRAIL-R1 antibody(ies) and the anti-TRAIL-R2 antibody(ies) and to reduce the toxicity imposed on normal cells. The present invention relates to recombinant obligate anaerobic Gram-positive bacteria that include a nucleic acid encoding a fusion protein having 3 or more anti-TRAIL-R1 single-chain antibodies and/or 3 or more anti-TRAIL-R2 single-chain antibodies, in an expressible state.
Hattori A.,Kyoto University |
Tsujimoto M.,Teikyo Heisei University
Journal of Biochemistry | Year: 2013
The human endoplasmic reticulum aminopeptidase (ERAP) 1 and 2 proteins were initially identified as homologues of human placental leucine aminopeptidase/insulin-regulated aminopeptidase. They are categorized as a unique class of proteases based on their subcellular localization on the luminal side of the endoplasmic reticulum. ERAPs play an important role in the N-terminal processing of the antigenic precursors that are presented on the major histocompatibility complex (MHC) class I molecules. ERAPs are also implicated in the regulation of a wide variety of physiological phenomena and pathogenic conditions. In this review, the current knowledge on ERAPs is summarized. © The Authors 2013. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
Teikyo Heisei University | Date: 2016-11-16
An object of the present invention is to effectively induce cancer cell apoptosis using the anti-TRAIL-R1 antibody(ies) and the anti-TRAIL-R2 antibody(ies) and to reduce the toxicity imposed on normal cells. The present invention relates to recombinant obligate anaerobic Gram-positive bacteria that comprise a nucleic acid encoding a fusion protein comprising 3 or more anti-TRAIL-R1 single-chain antibodies and/or 3 or more anti-TRAIL-R2 single-chain antibodies, in an expressible state.
Teikyo Heisei University | Date: 2015-05-20
An object of the present invention is to provide an antitumor agent and/or a marker for tumor detection with low invasiveness, few side effects, and high target specificity. Through the use of a recombinant obligate anaerobic gram-positive bacterium as an active ingredient containing a nucleic acid encoding a secretory fusion protein comprising a signal peptide, a low-molecular-weight single-chain antibody, and a functional peptide, an antitumor agent that enables delivery of a functional peptide to a target cell and/or a marker for tumor detection that enables monitoring of therapeutic effects over time are provided.