Entity

Time filter

Source Type

Laboratory, Japan

Nishijima M.,Marine Biotechnology Institute | Nishijima M.,TechnoSuruga Laboratory Co. | Adachi K.,Marine Biotechnology Institute | Katsuta A.,Marine Biotechnology Institute | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase-and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0-5.0% (w/v) NaCl (optimum of 2.0% NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were C18: 1∞7c, C16: 1∞7c and C16: 0 and the hydroxy acids were C10: 0 3-OH and C12: 0 3-OH. The DNA G+C content was 48.3-48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus Endozoicomonas in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5-96.9% 16S rRNA gene sequence similarity with Endozoicomonas elysicola MKT110T and Endozoicomonas montiporae CL-33T, with which the isolates formed a monophyletic cluster with 100% bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus Endozoicomonas, which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C14: 0 and C18: 1∞7c. Although some phenotypic differences were identified, the isolates should be assigned to the genus Endozoicomonas on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name Endozoicomonas numazuensis sp. nov. is proposed. The type strain is HC50T (=NBRC 108893T =DSM 25634T). An emended description of the genus Endozoicomonas is presented. © 2013 IUMS. Source


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped or spherical bacterium, strain 2A-7T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain produced a pigment(s), the absorption spectrum of which closely resembled that of b-carotene. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Flavobacteriaceae and clustered distantly with the type strains of species of the genus Lutibacter (up to 93.9% similarity). The DNA G+C content was 34.1 mol%. The major fatty acids were summed feature 3 (iso-C15: 02-OH and/or C16: 1ω7c), iso-C17: 03-OH and iso-C15: 03-OH. The major polar lipids were phosphatidylethanolamine and three unidentified lipids. Menaquinone 6 was detected as the sole isoprenoid quinone. On the basis of phenotypic, genotypic and chemotaxonomic data, strain 2A-7Trepresents a novel genus and species, for which the name Flavicella marina gen. nov., sp. nov. is proposed. The type strain of Flavicella marina is 2A-7T(=NBRC 110145T=KCTC 42197T). © 2015 IUMS. Source


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 5-11T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain exhibited a narrow growth temperature range of 20–30 6C. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the order Kordiimonadales in the class Alphaproteobacteria and was related most closely to the genus Kordiimonas (up to 91.2% similarity to the type strains of species of the genus) but branched deeply from species of Kordiimonas. The major fatty acids were iso-C17: 1ɷ9c, iso-C15: 0, and C16: 1ɷ7c and/or iso- C15: 0 2-OH. Ubiquinone-10 (Q-10) was detected as the sole isoprenoid quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. Although strains of Kordiimonas have been shown to contain unidentified glycolipids, they were not detected from strain 5-11T. The DNA G+C content of strain 5-11T was 44.3 mol%, a value that was lower than those of strains of Kordiimonas (50–58 mol%) and was relatively low for the members of the class Alphaproteobacteria. On the basis of phenotypic, genotypic and chemotaxonomic data, it is proposed that strain 5-11T represents a novel species of a new genus, Temperatibacter marinus gen. nov., sp. nov., within a new family Temperatibacteraceae fam. nov. The type strain of Temperatibacter marinus is 5-11T (=NBRC 110045T=LMG 28278T). © 2014 IUMS. Source


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 8-8T, was isolated from surface seawater at Muroto, Kochi, Japan. The strain exhibited agar-degrading activity. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Alteromonadaceae and clustered distantly with members of the genus Glaciecola (≤94.0 % similarity). The DNA G+C content was 41.8 mol%. The major fatty acids were C16: 1ω7c and/or iso-C15: 0 2-OH, C16: 0 and C18: 1ω7c and the major hydroxy fatty acid was C12: 0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified polar lipid; lysophosphatidylethanolamine and unidentified polar lipids were found as minor components. The major quinone was Q-8. On the basis of phenotypic, genotypic and chemotaxonomic data, strain 8-8T represents a novel species of a new genus, for which the name Agaribacter marinus gen. nov., sp. nov. is proposed. The type strain of Agaribacter marinus is 8-8T (=NBRC 110023T=LMG 28167T). © 2014 IUMS. Source


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped bacterium, designated strain 2-3T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. This strain grew well with starch. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Rhodobacteraceae and that the strain was related most closely to the genus Pacificibacter (94.0 % sequence similarity to the type strain). The DNA G+C content was 52.4 mol%. The major fatty acids were C18: 1ω7c, C14: 0 and C16: 0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The major isoprenoid quinone was Q-10. Strain 2-3T did not grow at 4 or 35 °C, while the type strain of the type species of the genus Pacificibacter grows at both temperatures. From the taxonomic data obtained in this study, it is proposed that strain 2-3T be placed into a novel genus and species named Amylibacter marinus gen. nov., sp. nov. in the family Rhodobacteraceae. The type strain ofAmylibacter marinus is 2-3T (= NBRC 110140T = LMG 28364T). © 2014 IUMS. Source

Discover hidden collaborations