TechnoSuruga Laboratory Co.

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TechnoSuruga Laboratory Co.

Laboratory, Japan
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University of Shizuoka, Technosuruga Laboratory Co. and Kendaigakari Co. | Date: 2016-07-13

It is an object to provide a simplified early diagnosis method for breast cancer, wherein a sample collected from a human subject is analyzed and determination of the presence or absence of breast cancer is made from the proportion of plural types of polyamines including N1-acetylspermidine.


Tazato N.,TechnoSuruga Laboratory Co. | Nishijima M.,TechnoSuruga Laboratory Co. | Handa Y.,TechnoSuruga Laboratory Co. | Kigawa R.,Independent Administrative Institution | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

Six Gram-negative, rod-shaped, non-spore-forming bacterial strains were isolated from small holes on plaster walls of the stone chamber interior of the Kitora Tumulus in Asuka village, Nara Prefecture, Japan. These were investigated by means of a polyphasic approach. All the isolates were strictly aerobic and motile by peritrichous flagella. Phylogenetic trees generated based on 16S rRNA gene sequences identified two novel lineages (comprising five isolates and one isolate, respectively) within the genus Gluconacetobacter. The isolates were characterized by having Q- 10 as the major ubiquinone system and C18: 1v7c (58.7-63.1% of the total) as the predominant fatty acid. DNA-DNA hybridization experiments endorsed the species rank for the two lineages, for which the names Gluconacetobacter tumulicola sp. nov. (type strain K=929-2-1bT5JCM 17774T=NCIMB 14760T) and Gluconacetobacter asukensis sp. nov. (type strain K8617-1- 1bT=JCM 17772T=NCIMB 14759T) are proposed. © 2012 IUMS.


Nishijima M.,Marine Biotechnology Institute | Nishijima M.,TechnoSuruga Laboratory Co. | Adachi K.,Marine Biotechnology Institute | Katsuta A.,Marine Biotechnology Institute | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase-and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0-5.0% (w/v) NaCl (optimum of 2.0% NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were C18: 1∞7c, C16: 1∞7c and C16: 0 and the hydroxy acids were C10: 0 3-OH and C12: 0 3-OH. The DNA G+C content was 48.3-48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus Endozoicomonas in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5-96.9% 16S rRNA gene sequence similarity with Endozoicomonas elysicola MKT110T and Endozoicomonas montiporae CL-33T, with which the isolates formed a monophyletic cluster with 100% bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus Endozoicomonas, which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C14: 0 and C18: 1∞7c. Although some phenotypic differences were identified, the isolates should be assigned to the genus Endozoicomonas on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name Endozoicomonas numazuensis sp. nov. is proposed. The type strain is HC50T (=NBRC 108893T =DSM 25634T). An emended description of the genus Endozoicomonas is presented. © 2013 IUMS.


Hisada T.,University of Shizuoka | Hisada T.,TechnoSuruga Laboratory Co. | Endoh K.,University of Shizuoka | Kuriki K.,University of Shizuoka
Archives of Microbiology | Year: 2015

Relationships between human gut microbiota, dietary habits, and health/diseases are the subject of epidemiological and clinical studies. However, the temporal stability and variability of the bacterial community in fecal samples remain unclear. In this study, middle-aged Japanese male and female volunteers (n = 5 each) without disease were recruited from the Sakura Diet Study. Fecal samples and lifestyle information were collected in every quarter and at each defecation for 7 continuous days. Next-generation sequencing of 16S rDNA and hierarchical clustering showed no time trend and intra-individual differences in both fecal sample sets. Significant inter-individual variations in seasonal and daily fecal sample sets were detected for 24 and 23 out of 39 selected dominant genera (>0.1 % of the total human gut microbiota; occupation rate >85 %), respectively. Intra- to inter-individual variance ratios in 26 and 35 genera were significantly <1.0 for seasonal and daily stabilities. Seasonal variation in fermented milk consumption might be associated with Bifidobacterium composition, but not with Lactobacillus. For most of the dominant genera in the human gut microbiota, inter-individual variations were significantly larger than intra-individual variations. Further studies are warranted to determine the contributions of human gut microbiota to nutritional metabolism, health promotion, and prevention/development of diseases. © 2015, The Author(s).


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 5-11T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain exhibited a narrow growth temperature range of 20–30 6C. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the order Kordiimonadales in the class Alphaproteobacteria and was related most closely to the genus Kordiimonas (up to 91.2% similarity to the type strains of species of the genus) but branched deeply from species of Kordiimonas. The major fatty acids were iso-C17: 1ɷ9c, iso-C15: 0, and C16: 1ɷ7c and/or iso- C15: 0 2-OH. Ubiquinone-10 (Q-10) was detected as the sole isoprenoid quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. Although strains of Kordiimonas have been shown to contain unidentified glycolipids, they were not detected from strain 5-11T. The DNA G+C content of strain 5-11T was 44.3 mol%, a value that was lower than those of strains of Kordiimonas (50–58 mol%) and was relatively low for the members of the class Alphaproteobacteria. On the basis of phenotypic, genotypic and chemotaxonomic data, it is proposed that strain 5-11T represents a novel species of a new genus, Temperatibacter marinus gen. nov., sp. nov., within a new family Temperatibacteraceae fam. nov. The type strain of Temperatibacter marinus is 5-11T (=NBRC 110045T=LMG 28278T). © 2014 IUMS.


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 8-8T, was isolated from surface seawater at Muroto, Kochi, Japan. The strain exhibited agar-degrading activity. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Alteromonadaceae and clustered distantly with members of the genus Glaciecola (≤94.0 % similarity). The DNA G+C content was 41.8 mol%. The major fatty acids were C16: 1ω7c and/or iso-C15: 0 2-OH, C16: 0 and C18: 1ω7c and the major hydroxy fatty acid was C12: 0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified polar lipid; lysophosphatidylethanolamine and unidentified polar lipids were found as minor components. The major quinone was Q-8. On the basis of phenotypic, genotypic and chemotaxonomic data, strain 8-8T represents a novel species of a new genus, for which the name Agaribacter marinus gen. nov., sp. nov. is proposed. The type strain of Agaribacter marinus is 8-8T (=NBRC 110023T=LMG 28167T). © 2014 IUMS.


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped bacterium, designated strain 2-3T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. This strain grew well with starch. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Rhodobacteraceae and that the strain was related most closely to the genus Pacificibacter (94.0 % sequence similarity to the type strain). The DNA G+C content was 52.4 mol%. The major fatty acids were C18: 1ω7c, C14: 0 and C16: 0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The major isoprenoid quinone was Q-10. Strain 2-3T did not grow at 4 or 35 °C, while the type strain of the type species of the genus Pacificibacter grows at both temperatures. From the taxonomic data obtained in this study, it is proposed that strain 2-3T be placed into a novel genus and species named Amylibacter marinus gen. nov., sp. nov. in the family Rhodobacteraceae. The type strain ofAmylibacter marinus is 2-3T (= NBRC 110140T = LMG 28364T). © 2014 IUMS.


Takahashi S.,TechnoSuruga Laboratory Co. | Tomita J.,TechnoSuruga Laboratory Co. | Nishioka K.,TechnoSuruga Laboratory Co. | Hisada T.,TechnoSuruga Laboratory Co. | Nishijima M.,TechnoSuruga Laboratory Co.
PLoS ONE | Year: 2014

For the analysis of microbial community structure based on 16S rDNA sequence diversity, sensitive and robust PCR amplification of 16S rDNA is a critical step. To obtain accurate microbial composition data, PCR amplification must be free of bias; however, amplifying all 16S rDNA species with equal efficiency from a sample containing a large variety of microorganisms remains challenging. Here, we designed a universal primer based on the V3-V4 hypervariable region of prokaryotic 16S rDNA for the simultaneous detection of Bacteria and Archaea in fecal samples from crossbred pigs (Landrace x Large white x Duroc) using an Illumina MiSeq next-generation sequencer. In-silico analysis showed that the newly designed universal prokaryotic primers matched approximately 98.0% of Bacteria and 94.6% of Archaea rRNA gene sequences in the Ribosomal Database Project database. For each sequencing reaction performed with the prokaryotic universal primer, an average of 69,330 (±20,482) reads were obtained, of which archaeal rRNA genes comprised approximately 1.2% to 3.2% of all prokaryotic reads. In addition, the detection frequency of Bacteria belonging to the phylum Verrucomicrobia, including members of the classes Verrucomicrobiae and Opitutae, was higher in the NGS analysis using the prokaryotic universal primer than that performed with the bacterial universal primer. Importantly, this new prokaryotic universal primer set had markedly lower bias than that of most previously designed universal primers. Our findings demonstrate that the prokaryotic universal primer set designed in the present study will permit the simultaneous detection of Bacteria and Archaea, and will therefore allow for a more comprehensive understanding of microbial community structures in environmental samples. © 2014 Takahashi et al.


Unno T.,Tokyo Kasei-Gakuin University | Hisada T.,TechnoSuruga Laboratory Company | Takahashi S.,TechnoSuruga Laboratory Company
Journal of Agricultural and Food Chemistry | Year: 2015

There has been particular interest in the prebiotic-like effects of commonly consumed polyphenols. This study aimed to evaluate the effects of hesperidin (HD) and its aglycone hesperetin (HT), major flavonoids in citrus fruits, on the structure and activity of gut microbiota in rats. Rats ingested an assigned diet (a control diet, a 0.5% HT diet, or a 1.0% HD diet) for 3 weeks. Terminal restriction fragment length polymorphism analysis revealed that the proportion of Clostridium subcluster XIVa in the feces collected at the third week of feeding was significantly reduced by the HT diet: 19.8 ± 4.3% for the control diet versus 5.3 ± 1.5% for the HT diet (P < 0.01). There was a significant difference in the cecal pool of short-chain fatty acids (SCFA), the sum of acetic, propionic, and butyric acids, between the control diet (212 ± 71 μmol) and the HT diet (310 ± 51 μmol) (P < 0.05), whereas the HD diet exhibited no effects (245 ± 51 μmol). Interestingly, dietary HT resulted in a significant increase in the excretion of starch in the feces. HT, but not HD, might reduce starch digestion, and parts of undigested starch were utilized to produce SCFA by microbial fermentation in the large intestine. © 2015 American Chemical Society.


Teramoto M.,Kochi University | Nishijima M.,TechnoSuruga Laboratory Co.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped or spherical bacterium, strain 2A-7T, was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain produced a pigment(s), the absorption spectrum of which closely resembled that of b-carotene. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Flavobacteriaceae and clustered distantly with the type strains of species of the genus Lutibacter (up to 93.9% similarity). The DNA G+C content was 34.1 mol%. The major fatty acids were summed feature 3 (iso-C15: 02-OH and/or C16: 1ω7c), iso-C17: 03-OH and iso-C15: 03-OH. The major polar lipids were phosphatidylethanolamine and three unidentified lipids. Menaquinone 6 was detected as the sole isoprenoid quinone. On the basis of phenotypic, genotypic and chemotaxonomic data, strain 2A-7Trepresents a novel genus and species, for which the name Flavicella marina gen. nov., sp. nov. is proposed. The type strain of Flavicella marina is 2A-7T(=NBRC 110145T=KCTC 42197T). © 2015 IUMS.

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