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Bejoy M.,Lawaharlal Nehru Tropical Botanic Garden and Research Institute | Dan M.,Lawaharlal Nehru Tropical Botanic Garden and Research Institute | Anish N.P.,Technology Bhavan | Nair A.R.G.,Lawaharlal Nehru Tropical Botanic Garden and Research Institute | And 2 more authors.
Biotechnology | Year: 2012

Curcuma vamana Sabu and Mangaly, a wild relative of tunneric, is endemic to Kerala and its germplasm deserves special conservation measures. A micropropagation protocol has been outlined for the production of complete plantlets within 85 days. Nodal segments isolated from stoloniferous rhizomes achieved 60% bud break within 20 days. These explants responded in BAP ( .0mg L ')supplemented medium with the sprouting of 1-3 shoots in 6-7 weeks of incubation. In vitro shoots obtained from primary cultures were used for further studies to minimise collection from the small wild populations of C. vamana. Efficient shoot multiplication has been obtained from in vitro shoot explants using thidiazuron (TDZ). The highest rate of shoot production was found in 0.5 mg L TDZ which produced 9.6 shoots. Rhizogenesis was observed along with collagenesis in all treatments. Effect of various nutrient formulations was also compared The best shoot multiplication response has been observed in Murashige and Skoog (MS) medium followed by Nitsch, Woody Plant (WPM), C (B5) and White's formulations. MS medium recorded about five-fold increase in the rate of shoot multiplication compared to that of the least responding medium under identical culture conditions. The regenerated plantlets were successfully established in the greenhouse condition with 90% survival. © 2012 Asian Network for Scientific Information.


Shukla S.K.,University of Delhi | Bharadvaja A.,University of Delhi | Parashar G.K.,University of Delhi | Mishra A.P.,Technology Bhavan | And 2 more authors.
Advanced Materials Letters | Year: 2012

Thin films of titanium dioxide in anatase form have been prepared using isobutyl titanate as precursor. The resulting TiO2 was coated on an U-shaped pyrex glass rod to sense the humidity of a controlled humid environment using optical fiber approach. The humidity sensing characteristics and the sensing mechanism have been investigated by measuring the output power of the sensor at different humidity. The developed humidity sensor was responded in the humidity ranging from 10 to 95% of relative humidity and exhibited the sensitivity of 0.78, response time 36s and recovery time 73s. © 2012 VBRI Press.


Bejoy M.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute | Amsh N.P.,Technology Bhavan | Radhika B.J.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute | Nair G.M.,Central University of Kerala
Biotechnology | Year: 2012

Ochlandra wightii (Munro) Fisch., an endemic reed bamboo in the Southern Western Ghats has been multiplied using tissue culture techniques. In vitro production of O. wightii plantlets has been achieved by using isolated embryos from mature seeds and nodal segments from in vitro seedlings. Better germination of isolated embryos (85%) was noticed in BAP (6-benzyl amino purine) (0.5 mg L-1) with 1-2 shoots in 30 days. Half-strength MS medium supplemented with BAP (0.5 mg L-1) andTDZ (Thidiazuron) (0.5 mg L-1) enhanced the rate of shoot multiplication to an average of 9.8 shoots in 60 days. Simultaneous development of roots along with shoots was also noticed. Rhizome induction was achieved from 65% of seedling cultures when treated with KN (Kinetin) (1 mg L-1) and higher concentration of sucrose (4%) in 35 days. Multiple shoots were also induced from nodal explants obtained from in vitro seedlings. Average 3.6 shoots were achieved in 40 days when treated with BAP (2 mg L-1) andKN (0.5 mg L-1). Twenty percent of the nodal cultures developed 1-2 roots during shoot development and the rest were rooted in half-strength MS solid medium supplemented with IBA (indole-3-butyric acid) (0.5 mg L-1). Plantlets with healthy roots were hardened and transplanted to the clay pots. They showed 80% survival after 6 months. However, the plantlets with in vitro rhizomes showed 100% survival. Development of new culms and rhizomes were noticed after 3 months of greenhouse growth. Around 880 plantlets could be generated in 9 months from 10 embryos isolated from mature seeds using this protocol. © 2012 Asian Network for Scientific Information.


Aggarwal N.,University of Delhi | Joshi B.,Technology Bhavan | Bhattacherjee A.B.,Jawaharlal Nehru University
Journal of Physics: Conference Series | Year: 2016

We investigate the effect of optomechanical coupling on the squeezed-spin states for a Bose-Einstein Condensate embedded within the lossless optomechanical cavity for the three special cases of initial states of cavity field, namely, a coherent state, a squeezed vacuum state and a squeezed state. We show that the radiation pressure or pondermotive force acting on the cavity end mirror plays a significant role in producing the atomic-squeezed states by producing squeezed states of the cavity field which is then transferred to the condensate. We further show that the maximum spin-squeezing along the x-direction is obtained in the presence of optomechanical coupling for the initial cavity field prepared in the amplitude squeezed state, whereas, squeezing along the y-direction reaches a maximum value in the absence of optomechanical coupling for the initial coherent cavity field. We also study the additional effect of nonlinear atomic interaction on spin-squeezing. © Published under licence by IOP Publishing Ltd.


Rana J.C.,National Bureau of Plant Genetic Resources Regional Station | Singh A.,National Bureau of Plant Genetic Resources Regional Station | Sharma Y.,National Bureau of Plant Genetic Resources Regional Station | Pradheep K.,National Bureau of Plant Genetic Resources Regional Station | Mendiratta N.,Technology Bhavan
Current Science | Year: 2010

The dynamics of plant bioresources including agriculture is different in the hills from those in the plains. The agriculture and forestry are the two major land use types and play an important role in providing food and livelihood security. The structure of vegetation for trees and shrubs layer was moderately instable and uneven as few species such as Acacia catechu, Lantana camara, Carissa spinarium in Mandhala; Quercus leucotrichophora, Pinus roxburghii, Myrsine africana in Moolbari, and Salix denticulata, Pinus wallichiana and Picea smithiana in Megad dominated the vegetation. Species richness was high at lower altitudes and low as we go higher. The distribution of species was mainly contiguous, however, few species showed random and regular distribution. Agricultural patterns have changed from traditional and subsistence to modern, which are primarily monoculture of high-valued cash crops. This has enhanced farm incomes but at the same time led to severe genetic erosion of traditional crops and varieties. Several development factors coupled with emerging climate change like erratic rain and snowfall patterns, flash floods, depletion of top soil and groundwater, destruction of natural habitat, wildlife menace, infestation of land through invasive alien weeds, low productivity, abandonment of agricultural lands have emerged as serious threats to the dynamics of hill agro-ecosystems.


Singhal S.,Panjab University | Namgyal T.,Panjab University | Laxhmi N.,University of Rajasthan | Bansal S.,Technology Bhavan
Scientia Iranica | Year: 2013

A cubic to tetragonal structure transition has evolved by substitution of Cu2+ ions in magnesium ferrite, fabricated via the sol-gel method. TEM studies revealed quasi-spherical particles for MgFe2 O4 and rod-shaped particles for CuFe2O4, suggesting an enhancement of morphological anisotropy with Cu doping. Thermal analysis shows the formation of MgFe2O4 and CuFe2 O4 at ∼380°C and ∼340°C, respectively, indicating a reduction in temperature for the formation of samples with copper doping. XRD shows the structural transition from a cubic to a tetragonal structure with increasing copper concentration. The dc electrical resistivity decreases with copper doping in MgFe2O4, attributing to the increase in conductivity due to Cu2+-Cu+ ions hopping. MgFe2 O4 has been found to have a saturation magnetization of ∼23 emu/g and a coercive field of ∼54 Oe. The saturation magnetization increases, while coercivity initially decreases and then increases with increasing copper concentration. © 2013 Sharif University of Technology. All rights reserved.


Bisoi R.,Siksha ‘O’ Anusandhan University | Reddy J.B.V.,Technology Bhavan
International Journal of Power and Energy Conversion | Year: 2014

This work presents a new technique for the identification of multiple power quality disturbances in industrial power systems. This new technique is developed based on change detection filter (CDF) and machine intelligence techniques like the local linear radial basis function neural network (LLRBFNN) and decision tree (DT). The proposed change detection filter has been designed in one cycle back fashion for the multiple non-stationary transient power signal disturbances for localisation, and feature extraction. The extracted features are fed as inputs to either a LLRBFNN or a decision tree-based classifier (DTC) for characterisation of the power quality events. The performance of LLRBFNN and DTC are evaluated and compared with local linear wavelet neural network (LLWNN), wavelet neural network (WNN), radial basis function neural network (RBFNN), etc., to highlight the superior performance of the proposed classifiers. Copyright © 2014 Inderscience Enterprises Ltd.


Misra P.K.,Sambalpur University | Dash U.,Aronai Academy | Dash R.,Technology Bhavan | Mandal A.B.,CSIR - Central Leather Research Institute
Journal of Molecular Liquids | Year: 2014

The interaction between a novel organic molecule (3E, 5E)-bis(4-butyloxy)benzylidene-8-(4-butyloxy)phenyldicyclopentano[2,3-b:3,2-e]pyridine(BP4) independently with a cationic surfactant, cetyltrimethylammonium bromide (CTAB) as well as with a nonionic surfactant, Triton-X-100 (TX-100) has been investigated through a number of analytical techniques such as conductivity, spectral analyses (fluorescence, UV-visible absorbance) and area/molecule measurements (Langmuir-Blodgett film method) in solution and at air-water interface. Both CTAB and TX-100 aggregate at lower concentration in the presence of BP4compared to the absence of later, indicating the generation of synergism in the solution mixture. The hydrocarbon chain of BP4interacts with the cetyl chain of CTAB or 1,1,3,3-teramethyl butyl chain of TX-100 through hydrophobic interaction thereby enhancing the surface activity of the mixture. Subsequently, CMC decreases. On the contrary, CMC of BP4increases upon addition of both CTAB and TX-100 due to the inefficiency of BP4to interact aptly with the CTAB/TX-100 to form hydrophobic complex (U. Dash, P. K. Misra, JCIS, 357(2011)407-418). But both the surfactants and BP4adsorb as monomer in the presence of each other and form a mixed monolayer at the air-water interface by associating through lateral interaction of their hydrocarbon chain. This leads to an increase of the cooperative effect as evidenced by the decrease in area per molecule at the air-water interface. © 2014 Published by Elsevier B.V.


Banerjee P.,CSIR - Central Electrochemical Research Institute | Singh V.S.,CSIR - Central Electrochemical Research Institute | Chatttopadhyay K.,Satyam Computer Services Ltd. | Chandra P.C.,Central Ground Water Board | Singh B.,Technology Bhavan
Journal of Hydrology | Year: 2011

The paper evaluates the prospect of artificial neural network (ANN) simulation over mathematical modeling in estimating safe pumping rate to maintain groundwater salinity in island aquifers. Feed-forward ANN model with quick propagation (QP) as training algorithm has been used to forecast the salinity under varied pumping rates. The accuracy, generalization ability and reliability of the model are verified by real-time field data. The model is trained with 2 years of real-time field data and prediction on water quality with varying pumping rate is made for a span of 5 years. The same is then compared with both real-time field data and the prediction based on SUTRA (Saturated-Unsaturated Transport) computations. The proposed ANN model has surfaced as a simpler and more accurate alternative to the numerical method techniques. The ANN methodology using minimal lag and number of hidden nodes, along with the optimal number of spatial and temporal variables consistently produced the best performing network based simulation models. The prediction accuracy of the ANN model has been extended for another 5 years to further define the limit of pumping at a permissible level of groundwater salinity. © 2010 Elsevier B.V.


Kumar S.,Jawaharlal Nehru University | Kumar S.,Hemwati Nandan Bahuguna Garhwal University | Kumar N.,Jawaharlal Nehru University | Alam N.,Technology Bhavan | Gourinath S.,Jawaharlal Nehru University
Biochimica et Biophysica Acta - Proteins and Proteomics | Year: 2014

Brucella abortus is the major cause of premature foetal abortion in cattle, can be transmitted from cattle to humans, and is considered a powerful biological weapon. De novo cysteine biosynthesis is one of the essential pathways reported in bacteria, protozoa, and plants. Serine acetyltransferase (SAT) initiates this reaction by catalyzing the formation of O-acetylserine (OAS) using l-serine and acetyl coenzyme A as substrates. Here we report kinetic and crystallographic studies of this enzyme from B. abortus. The kinetic studies indicate that cysteine competitively inhibits the binding of serine to B. abortus SAT (BaSAT) and noncompetitively inhibits the binding of acetyl coenzyme A. The crystal structures of BaSAT in its apo state and in complex with coenzyme A (CoA) were determined to 1.96 Å and 1.87 Å resolution, respectively. BaSAT was observed as a trimer in a size exclusion column; however, it was seen as a hexamer in dynamic light scattering (DLS) studies and in the crystal structure, indicating it may exist in both states. The complex structure shows coenzyme A bound to the C-terminal region, making mostly hydrophobic contacts from the center of the active site extending up to the surface of the protein. There is no conformational difference in the enzyme between the apo and the complexed states, indicating lock and key binding and the absence of an induced fit mechanism. © 2014 Elsevier B.V.

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