PubMed | University of Houston, Center for Technological Transfer, King Fahd University of Petroleum and Minerals and Italian National Cancer Institute
Type: Journal Article | Journal: Oncotarget | Year: 2016
We synthesized, characterized and tested in a panel of cancer cell lines, nine new bipyridine gold(III) dithiocarbamate-containing complexes. In vitro studies demonstrated that compounds 1, 2, 4, 5, 7 and 8 were the most cytotoxic in prostate, breast, ovarian cancer cell lines and in Hodgkin lymphoma cells with IC50 values lower than the reference drug cisplatin. The most active compound 1 was more active than cisplatin in ovarian (A2780cis and 2780CP-16) and breast cancer cisplatin-resistant cells. Compound 1 determined an alteration of the cellular redox homeostasis leading to increased ROS levels, a decrease in the mitochondrial membrane potential, cytochrome-c release from the mitochondria and activation of caspases 9 and 3. The ROS scavenger NAC suppressed ROS generation and rescued cells from damage. Compound 1 resulted more active in tumor cells than in normal human Mesenchymal stromal cells. Gold compounds were active independent of p53 status: exerted cytotoxic effects on a panel of non-small cell lung cancer cell lines with different p53 status and in the ovarian A2780 model where the p53 was knocked out. In conclusion, these promising results strongly indicate the need for further preclinical evaluation to test the clinical potential of these new gold(III) complexes.
Tamburini M.,Center for Technological Transfer |
Maresi G.,Center for Technological Transfer |
Salvadori C.,Center for Technological Transfer |
Battisti A.,University of Padua |
And 2 more authors.
Bulletin of Insectology | Year: 2012
Non-native organisms can affect native communities and ecosystems in different ways. We examine here the case of the western conifer seed bug Leptoglossus occidentalis Heidemann (Heteroptera Coreidae), a polyphagous pest of conifer seeds, introduced from northern America into Italy in 1999 and then spreading across the whole Europe. The bug was detected in alpine forests of Trentino (northern Italy) in 2002, and since then known mainly as a nuisance agent for its habit to overwinter inside buildings. The lack of information on the ecology in mountain areas led us to investigate its distribution in some alpine pine stands, in relation to altitude and to fungal pathogens potentially associated. The presence of L. occidentalis was observed in all main geographic areas of Trentino, up to the subalpine belt in the southern part of the region. The seed bug completed one or two generations depending on altitude and local climatic conditions. Field data and rearing under artificial conditions indicated the importance of heat accumulation for the development of nymphal instars and the role of temperature thresholds in regulating adult behaviour. The seed bug was observed on trees (Pinus nigra, P. sylvestris) as well as on shrubs (P. mugo), facilitating the rapid colonisation of a fragmented mountain environment. This may have important implication in the dispersal of pathogens, as spores of Diplodia pinea were detected on adults. Although a direct economic impact has not been evidenced yet in this alpine area, an ecological impact hampering natural regeneration, especially in high-altitude forest ecosystems, can be envisaged.