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Ojeda-Chi M.M.,Autonomous University of Yucatan | Rodriguez-Vivas R.I.,Autonomous University of Yucatan | Galindo-Velasco E.,University of Colima | Lezama-Gutierrez R.,University of Colima | Cruz-Vazquez C.,Technological Institute of the Llano de Aguascalientes
Revista Mexicana De Ciencias Pecuarias | Year: 2011

Infestations with cattle tick, Rhipicephalus microplus constitute the most important ectoparasite problem in the tropical and subtropical regions of the globe, resulting in major economic losses in the production of beef, milk, and leathers, in addition of increasing the cost of control programs transmitting Babesia bovis, B. bigemina and Anaplasma marginale. The control of R. microplus is mostly based on the use of ixodicides. Nevertheless, the irrational use of such products has resulted in tick populations exhibiting resistance to all major ixodicide drug classes. This has urged the development of non-chemical control alternatives, including the use of entomopathogenic fungi, among which Metarhizium anisopliae (Hypocreales: Clavicipitaceae) has shown to be efficient both in vitro and in vivo for the control of the different evolution stages of R. microplus. The use of these fungi results in decreased oviposition, increased incubation/hatch times, and death of tick larval and adult stages, with efficiency rates of up to 100 %. Several studies show that M. anisopliae is a sustainable non-chemical alternative for the control of ticks. The purpose of this paper is to present an updated review on the use of different M. anisopliae strains for the control of R. microplus.

Tirado-Estrada G.,Autonomous University of Aguascalientes | Tirado-Estrada G.,Technological Institute of the Llano de Aguascalientes | Mendoza-Martinez G.D.,Metropolitan Autonomous University | Pinos-Rodriguez J.M.,Autonomous University of San Luis Potosi | And 2 more authors.
Journal of Applied Animal Research | Year: 2011

The objective of this study was to compare the effects of two fibrolytic enzyme mixtures (Fibrozyme and Promote) on growth performance, digestion and ruminal fermentation in lambs fed corn stover based diets. Forty-two crossbred (Black Belly × Dorper) lambs (20±1.6 kg) were randomly assigned to three treatments defined as Control (no enzymes), Fibrozyme (Alltech, Nicholasville, KY, USA), and Promote (Cargill Corp., Minneapolis, MN, USA). Growth performance evaluation lasted 55 days. On day 55, rumen fluid samples were obtained. Both Fibrozyme and Promote improved growth performance, feed conversion and digestion as compared to Control. Enzymes increased total volatile fatty acids, but only Fibrozyme increased molar proportion of propionate, decreased molar proportion of butyrate, and reduced the acetate: propionate ratio. It is concluded that both Fibrozyme and Promote improved growth performance and digestion in lambs fed highforage diets. © 2011 Taylor & Francis.

Galindo-Velasco E.,University of Colima | Lezama-Gutierrez R.,University of Colima | Cruz-Vazquez C.,Technological Institute of the Llano de Aguascalientes | Pescador-Rubio A.,University of Colima | And 4 more authors.
Veterinary Parasitology | Year: 2015

The purpose of this paper is to evaluate the effect of five strains of Metarhizium anisopliae (Ma) and three strains of Isaria fumosorosea (Ifr) at a concentration of 1 × 108colonyformingunits/ml applied by spraying onto bovines with controlled infestation of Haematobia irritans under stable conditions in the Mexican dry tropics. Four experiments were performed, in each of which three treatments (two fungal strains and one control) were evaluated with eight repetitions for each one, by carrying out a single application of the aqueous suspension of each strain. The animals were isolated in individual cages and direct counts of the infestation were carried out for 13 days. It was observed that strains Ma2, Ma6, Ma10, Ma14, and Ma34 caused 94-100% reduction in infestation between days 12 and 13 post-treatment, while strains Ifr19, Ifr11, and Ifr12 reduced infestation from 90% to 98% up to day 13 post-application. There was an effect in the generation of horn flies from the excrement of bovines that were treated with different strains, reducing the reproduction of subsequent generations. It was concluded that the strains of M. anisopliae and I. fumosorosea evaluated in this study can be used as biocontrol agents in infestations of H. irritans in stabled bovines. © 2015 Elsevier B.V.

Zapata-Vazquez A.,Technological Institute of the Llano de Aguascalientes
TheScientificWorldJournal | Year: 2012

Chili pepper blight observed on pepper farms from north Aguascalientes was monitored for the presence of Phytophthora capsici during 2008-2010. Initially, ELISA tests were directed to plant samples from greenhouses and rustic nurseries, showing an 86% of positive samples. Later, samples of wilted plants from the farms during the first survey were tested with ELISA. The subsequent survey on soil samples included mycelia isolation and PCR amplification of a 560 bp fragment of ITS-specific DNA sequence of P. capsici. Data was analyzed according to four geographical areas defined by coordinates to ease the dispersal assessment. In general, one-third of the samples from surveyed fields contained P. capsici, inferring that this may be the pathogen responsible of the observed wilt. Nevertheless, only five sites from a total of 92 were consistently negative to P. capsici. The presence of this pathogen was detected through ELISA and confirmed through PCR. The other two-thirds of the negative samples may be attributable to Fusarium and Rhizoctonia, both isolated instead of Phytophthora in these areas. Due to these striking results, this information would be of interest for local plant protection committees and farmers to avoid further dispersal of pathogens to new lands.

Beas R.C.,Technological Institute of the Llano de Aguascalientes | Batista-Galvez A.,Juarez Autonomous University of Tabasco
Acta Horticulturae | Year: 2010

The induction of callus formation was done from three different explants: leaf, hypocotyls and embryos, in combination with 24 formulations with 2,4-D (2,4-dichlorophenoxyacetic acid), BA (benzyladenine), NAA (1-naphthaleneacetic acid) and K (kinetin). The results show a great diversity of callus formation types. The value of logistic regression was significant for explants from leaves and hypocotyls. Callus induction occurred in 31 of 75 treatments with leaf and hypocotyl explants considered the best material for callus induction in guava.

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