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Bohoyo-Gil D.,Agri Food Technological Institute INTAEX | Dominguez-Valhondo D.,Agri Food Technological Institute INTAEX | Garcia-Parra J.J.,Agri Food Technological Institute INTAEX | Gonzalez-Gomez D.,Agri Food Technological Institute INTAEX | Gonzalez-Gomez D.,Spanish University for Distance Education (UNED)
European Food Research and Technology | Year: 2012

The purpose of this paper was to optimize an ultra-high performance liquid chromatography (UHPLC) method for the determination and quantification of carotenoid pigments in food products and compare it with a HPLC method in terms of resolution, selectivity, sensitivity, time and reagent saving. Less analysis times, solvent consumption and better peak resolution were achieved when the UHPLC methodology was used. Ultra-high performance liquid chromatography analysis was performed on Zorbax Eclipse Plus C18 column (2. 1 × 50 mm; 1. 8 μm) using a gradient solvent system and diode array detector. A total of seven carotenoids were separated and quantified in <8. 5 min. For all components, the resolution was higher than 1. 5 and LOD was below 1. 0 μg mL-1. To determine the intraday and reproducibility, assays were conducted in 10 continuous days for method validation achieving recuperation rates ranging between 85 and 100 % and no significant differences among the results obtained in same and different days. The proposed methodology was applied for the determination of the carotenoid contents in honeybee pollen samples, pumpkin and nectarine flesh with satisfactory recovery. © 2012 Springer-Verlag Berlin Heidelberg. Source

Dominguez-Valhondo D.,Agri Food Technological Institute INTAEX | Gonzalez-Gomez D.,Agri Food Technological Institute INTAEX | Hernandez-Mendez T.,Agri Food Technological Institute INTAEX | Bohoyo-Gil D.,Agri Food Technological Institute INTAEX
Food Science and Technology International | Year: 2013

Honeybee-collected pollen constitutes a potential source of energy and proteins for human consumption. The aim of this study was to determinate if the volatile fraction of pollen is affected by the application of industrial treatment processes and by the floral origin. Two process (thermal and freeze-drying) were compared with the fresh product. The volatile composition was investigated by a suitable analytical procedure based on dynamic headspace followed by thermal desorption gas chromatography mass spectrometry. Using this methodology more than 60 volatile compounds were separated in a phenyl-methyl-siloxane 50 m chromatographic column and identified according to their mass spectra, Kovats retention indices and comparison with those of authenticated reference standards. The differences among the studied honeybee pollens, in terms on volatile profile, were studied by means of the analysis of variance and principal component analysis. The volatile profile of fresh pollen is influenced by the floral origin and altered when it is processed, either by thermal and freeze-drying process, but when thermal process was used, compounds as dimethyl sulphyde and furfural, which affect negatively the functional quality, are found. As conclusion, the freeze-drying system could be a good alternative to the traditional thermal drying process. © SAGE Publications 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav. Source

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