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Andino J.E.G.,University of California at Berkeley | Andino J.E.G.,Instituto Nacional Of Biodiversidad | Fernandez-Fernandez D.,Instituto Nacional Of Biodiversidad | Palacios W.A.,Instituto Nacional Of Biodiversidad | Palacios W.A.,Technical University of the North, Ibarra
Phytotaxa | Year: 2016

Sloanea multinervis, a new species collected in the terra firme forests of the Ecuadorean Amazon, is described and illustrated, and its morphological similarities with other species of Sloanea are discussed. © 2016 Magnolia Press.


Benito-Pescador D.,University of Salamanca | Santander D.,University of Salamanca | Santander D.,Technical University of the North, Ibarra | Arranz M.,University of Salamanca | And 4 more authors.
Frontiers in Microbiology | Year: 2016

Botrytis cinerea is a widespread necrotrophic fungus which infects more than 200 plant species. In an attempt to characterize the physiological status of the fungus in planta and to identify genetic factors contributing to its ability to infect the host cells, a differential gene expression analysis during the interaction B. cinerea-tomato was carried out. Gene Bcmimp1 codes for a mRNA detected by differential display in the course of this analysis. During the interaction with the host, it shows a transient expression pattern with maximal expression levels during the colonization and maceration of the infected tissues. Bioinformatic analysis suggested that BCMIMP1 is an integral membrane protein located in the mitochondrial inner membrane. Co-localization experiments with a BCMIMP1-GFP fusion protein confirmed that the protein is targeted to the mitochondria. ΔBcmimp1 mutants do not show obvious phenotypic differences during saprophytic growth and their infection ability was unaltered as compared to the wild-type. Interestingly, the mutants produced increased levels of reactive oxygen species, likely as a consequence of disturbed mitochondrial function. Although Bcmimp1 expression is enhanced in planta it cannot be considered a pathogenicity factor. © 2016 Benito-Pescador, Santander, Arranz, Díaz-Mínguez, Eslava, van Kan and Benito.


Palacios W.,Technical University of the North, Ibarra
Caldasia | Year: 2012

Four new tree species of Ecuador belonging to four families are described: Carapa amorphocarpa (Meliaceae) and Hasseltia crassa (Salicaceae) from the slopes of the Cerro Golondrinas, near the border with Colombia; Gloeospermum crassicarpum (Violaceae) from shared border of Colombia, Ecuador, and Peru; and Gyranthera amphibiolepis (Malvaceae) from the foothills of eastern Ecuador. Carapa amorphocarpa is distinguished easily by its thick leaflets with rounded or shortly emarginated apices and especially by its enormous amorphous fruits. Hasseltia crassa is distinguished by its large, coriaceous, thick leaves with deeply impressed venation and the inflorescence, which is relatively short and robust. Gloeospermum crassicarpum is characterized by the corolla, which is orange and apically curved, and the fruit, which has a thick mesocarp. Gyranthera amphibiolepis is characterized by the digitate leaves and the flowers, which are up to 8 cm in length, and have a staminal tube that shows the anthers in a similar arrangement to that of Ochroma (Malvaceae).


Trujillo L.E.,University las Fuerzas Armadas | Avalos R.,University las Fuerzas Armadas | Granda S.,University las Fuerzas Armadas | Guerra L.S.,University las Fuerzas Armadas | Pais-Chanfrau J.M.,Technical University of the North, Ibarra
Biology and Medicine | Year: 2016

Nanoscience is an emerging technology today with great application in different fields. Biocatalysts design, identification of different bacterial strains, monitoring the quality of food by different biosensors, food packaging with smart systems, active, intelligent and nano-encapsulation of bioactive food compounds are examples of some of these applications in the food industry. In this paper, some topics related to the potential of nanotechnology in the food industry are updated. In addition, some concerns about nanotechnology application in this popular industry are also discussed. © 2016 Trujillo LE, et al.


Pais-Chanfrau J.M.,Technical University of the North, Ibarra | Trujillo-Toledo L.E.,University las Fuerzas Armadas
Biology and Medicine | Year: 2016

The methylotrophic yeast Pichia pastoris became an excellent and successfully expression system for therapeutics and other useful heterologous proteins at large-scale. Its capacity to reach a high-cell-densityconcentration during its fed-batch fermentations in a chemically-defined culture medium is one of the main features of this system. The standard culture medium BSM developed by Invitrogen Co. has been the most widespread used. However, it contains certain components in improper ratios that lead to precipitation of some of its components during preparation, mainly after sterilization and/or during pH adjustment. In this study, we propose a modified version of the BSM culture medium, named MBSM. These simple modifications by employing a linear optimization technique solves the precipitation problems observed on BSM and other culture media. Beside it, is rather simple, easy to prepare, and inexpensive, and it has impact positively over the environment by reducing the pollution load of the fermentation waste and also diminish the cost of the industrial scale fermentation process. By using this modified medium, were reached cell densities of 100 gDCW · L-1 and 60 gDCW · L-1 for mini-proinsulin and exo-levanase under the inducible AOX1 and the constitutive GAPDH promoters, respectively. These results allow to get vast amounts of proteins able to be used in nanoscience and other biotechnological applications. © 2016 Pais-Chanfrau JM, et al.

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