Tecan Schweiz AG

Switzerland

Tecan Schweiz AG

Switzerland
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TECAN Schweiz AG | Date: 2016-07-14

A relative humidity control apparatus for control of the relative humidity in a gas space has a nebulizer source with an outlet for nebulized liquid, a frame surrounding an open area and comprising an opening arrangement to the open area and in operational flow connection with the outlet, and a flow drive arrangement generating a gas flow from the outlet to and out of the opening arrangement. A liquid handling robot comprising this apparatus, a method of operating the apparatus, an immunoassay method and methods of controlling the time course of the relative humidity in a gaseous space and of producing a predetermined volume of a liquid are also disclosed.


Hadener M.,University of Bern | Weinmann W.,University of Bern | Van Staveren D.R.,Tecan Schweiz AG | Konig S.,University of Bern
Bioanalysis | Year: 2017

Aim: Generally, urine drug testing for cannabis abuse involves measuring total concentrations of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCCOOH) obtained by enzymatic and/or alkaline hydrolysis of THCCOOH-glucuronide. As hydrolysis can be inconsistent and incomplete, direct measurement of the two metabolites is preferable. Methodology & results: We developed a high-throughput LC-MS/MS method for simultaneous quantification of free and glucuronidated THCCOOH in urine using coated 96-well plates for analyte extraction and column-switching chromatography. Excellent separation of the two analytes was achieved within 2.5 min, with linear ranges from 5 to 2000 μg/l for THCCOOH and from 10 to 4000 μg/l for THCCOOH-glucuronide. Conclusion: The method was successfully validated and applied to authentic urine samples from cannabis consumers, demonstrating its applicability for routine cannabinoid testing. © 2017 Future Science Ltd.


Almario J.,University Claude Bernard Lyon 1 | Almario J.,CNRS Microbial Ecology | Gobbin D.,ETH Zurich | Gobbin D.,Research and Innovation Center | And 6 more authors.
Research in Microbiology | Year: 2014

Functional type III secretion system (T3SS) genes are needed for effective biocontrol of Pythium damping-off of cucumber by Pseudomonas fluorescens KD, but whether biocontrol Pseudomonas strains with T3SS genes display overall a higher plant-protecting activity is unknown. The assessment of 198 biocontrol fluorescent pseudomonads originating from 60 soils worldwide indicated that 32% harbour the ATPase-encoding T3SS gene hrcN, which was most often found in tomato isolates. The hrcN+ biocontrol strains (and especially those also producing 2,4-diacetylphloroglucinol and displaying 1-aminocyclopropane-1-carboxylate deaminase activity) displayed higher plant-protecting ability in comparison with hrcN- biocontrol strains, both in the Pythium/cucumber and Fusarium/cucumber pathosystems. © 2014 Institut Pasteur.


Beck J.,ETH Zurich | Maerki S.,ETH Zurich | Maerki S.,Tecan Schweiz AG | Posch M.,University of Dundee | And 10 more authors.
Nature Cell Biology | Year: 2013

Polo-like kinase 1 (PLK1) critically regulates mitosis through its dynamic localization to kinetochores, centrosomes and the midzone. The polo-box domain (PBD) and activity of PLK1 mediate its recruitment to mitotic structures, but the mechanisms regulating PLK1 dynamics remain poorly understood. Here, we identify PLK1 as a target of the cullin 3 (CUL3)-based E3 ubiquitin ligase, containing the BTB adaptor KLHL22, which regulates chromosome alignment and PLK1 kinetochore localization but not PLK1 stability. In the absence of KLHL22, PLK1 accumulates on kinetochores, resulting in activation of the spindle assembly checkpoint (SAC). CUL3-KLHL22 ubiquitylates Lys 492, located within the PBD, leading to PLK1 dissociation from kinetochore phosphoreceptors. Expression of a non-ubiquitylatable PLK1-K492R mutant phenocopies inactivation of CUL3-KLHL22. KLHL22 associates with the mitotic spindle and its interaction with PLK1 increases on chromosome bi-orientation. Our data suggest that CUL3-KLHL22-mediated ubiquitylation signals degradation-independent removal of PLK1 from kinetochores and SAC satisfaction, which are required for faithful mitosis. © 2013 Macmillan Publishers Limited. All rights reserved.


Weibel R.,ZHAW Zurich University of Applied Sciences | Iten M.,ZHAW Zurich University of Applied Sciences | Konig I.,ZHAW Zurich University of Applied Sciences | Beckbissinger R.,Hamilton Bonaduz AG | And 6 more authors.
JALA - Journal of the Association for Laboratory Automation | Year: 2010

Robotic liquid-handling systems using fixed reusable pipetting tips are used not only in fully integrated in vitro clinical diagnostic analyzers, but also in open platform robotic liquid-handling systems. These are not confined to particular diagnostic assays, as customers adapt a large variety of assays on these platforms. One major problem with the use of fixed, reusable tips is the carry over (CO) of analyte from sample to sample. Despite widespread use of fixed tips in open platform systems, systematic studies on procedures to quantify CO for analytes other than nucleic acids are missing. In a consortium with three liquid-handling system suppliers and one coating specialist, we developed test procedures for the quantification of CO. The procedures were standardized and tested with the analytes fluorescein, immunoglobulin G, and hepatitis B surface antigen as model substances for small organic molecules, antibodies, and complex biomolecules. The test procedures allow the reproducible quantification of the CO with intra- and interassay precisions of less than 6% coefficient of variation. They were used to investigate the effect of different tip coatings on the CO of the three analytes. Fluoropolymers, inorganic-organic nanocomposites, sodium-silicate glass, titanium dioxide, and silicone resins, which are used in special applications, showed only small differences in CO. The CO test procedures can be easily transferred to different liquid-handling systems and used with different analytes. © 2010 Society for Laboratory Automation and Screening.


Iten M.,ZHAW Zurich University of Applied Sciences | Weibel R.,ZHAW Zurich University of Applied Sciences | Konig I.,ZHAW Zurich University of Applied Sciences | Beckbissinger R.,Hamilton Bonaduz AG | And 6 more authors.
JALA - Journal of the Association for Laboratory Automation | Year: 2010

Robotic liquid-handling systems can be equipped with disposable pipetting tips or fixed reusable pipetting tips. The use of disposable tips is perceived as the best option to avoid carry over (CO) of analyte from sample to sample. We recently developed standardized CO test procedures that allow precise and reproducible quantification of CO for fixed reusable tips. We used these test procedures to reduce CO of the analytes fluorescein, IgG, and hepatitis B surface antigen (HBsAg) to minimal levels. Variations of washing intensity, using water as a washing solution, did not lower CO below acceptable target levels. These target levels would preclude a false-positive detection of IgG and HBsAg in human serum when a negative sample is measured subsequent to a sample with a high analyte concentration. We therefore integrated a decontamination step into the washing procedure. Screening of 12 decontamination solutions showed that sodium hypochlorite solution was very efficient in reducing CO. Optimization led to a final washing routine in which tips are exposed for 0.2. s to 0.17. M NaOCl and subsequently rinsed with 2. mL of water to remove any remaining decontaminant solution. The washing procedure only takes 15. s and is thus suitable for high-throughput applications. The procedure was able to lower CO of IgG and HBsAg in human sera below relevant levels. The decontamination step with hypochlorite can easily be integrated into different liquid-handling systems and is likely to be effective against CO of most proteins and peptides. © 2010 Society for Laboratory Automation and Screening.


Rimann M.,ZHAW Zurich University of Applied Sciences | Angres B.,Cellendes GmbH | Patocchi-Tenzer I.,Tecan Schweiz AG | Braum S.,Tecan Schweiz AG | Graf-Hausner U.,ZHAW Zurich University of Applied Sciences
Journal of Laboratory Automation | Year: 2014

Drug development relies on high-throughput screening involving cell-based assays. Most of the assays are still based on cells grown in monolayer rather than in three-dimensional (3D) formats, although cells behave more in vivo-like in 3D. To exemplify the adoption of 3D techniques in drug development, this project investigated the automation of a hydrogel-based 3D cell culture system using a liquid-handling robot. The hydrogel technology used offers high flexibility of gel design due to a modular composition of a polymer network and bioactive components. The cell inert degradation of the gel at the end of the culture period guaranteed the harmless isolation of live cells for further downstream processing. Human colon carcinoma cells HCT-116 were encapsulated and grown in these dextran-based hydrogels, thereby forming 3D multicellular spheroids. Viability and DNA content of the cells were shown to be similar in automated and manually produced hydrogels. Furthermore, cell treatment with toxic Taxol concentrations (100 nM) had the same effect on HCT-116 cell viability in manually and automated hydrogel preparations. Finally, a fully automated dose-response curve with the reference compound Taxol showed the potential of this hydrogel-based 3D cell culture system in advanced drug development. © 2013 Society for Laboratory Automation and Screening.


Franscini N.,ZHAW Zurich University of Applied Sciences | Wuertz K.,University of Zürich | Patocchi-Tenzer I.,Tecan Schweiz AG | Durner R.,Tecan Schweiz AG | And 2 more authors.
Journal of Laboratory Automation | Year: 2011

Implementation of regenerative medicine in the clinical setting requires not only biological inventions, but also the development of reproducible and safe method for cell isolation and expansion. As the currently used manual techniques do not fulfill these requirements, there is a clear need to develop an adequate robotic platform for automated, large-scale production of cells or cell-based products. Here, we demonstrate an automated liquid-handling cell-culture platform that can be used to isolate, expand, and characterize human primary cells (e.g., from intervertebral disc tissue) with results that are comparable to the manual procedure. Specifically, no differences could be observed for cell yield, viability, aggregation rate, growth rate, and phenotype. Importantly, all steps-from the enzymatic isolation of cells through the biopsy to the final quality control-can be performed completely by the automated system because of novel tools that were incorporated into the platform. This automated cell-culture platform can therefore replace entirely manual processes in areas that require high throughput while maintaining stability and safety, such as clinical or industrial settings. © 2011 Society for Laboratory Automation and Screening.


Baecher S.,Ludwig Maximilians University of Munich | Geyer R.,Tecan Schweiz AG | Lehmann C.,Tecan Schweiz AG | Vogeser M.,Ludwig Maximilians University of Munich
Clinical Chemistry and Laboratory Medicine | Year: 2014

Already available sample preparation technologies for liquid chromatography-tandem mass spectrometry have substantial shortcomings with respect to automation. A novel approach is based on gel-like polymeric material with defined absorption chemistry, which is immobilized in micro-plate wells. It is referred to as Tecan Immobilized Coating Extraction™ (TICE™) technology and it enables easy automation on liquid handling systems. We aimed to study the performance of Tecan AC Extraction Plate™ based on this principle by addressing 25-hydroxyvitamin D (25OHD) as an exemplary analyte. Methods: A protocol for extraction of 25OHD from serum samples based on TICE™ technology was implemented on a robotic liquid handling system Freedom EVO® (Tecan). An isotope-dilution ultra-performance liquid chromatography- tandem mass spectrometry method was used for quantification. Performance was tested according to a comprehensive protocol. Results: Linearity was found over a range from 4.3 to 65.8 ng/mL for 25OHD3. The coefficients of variation for the intra-day and inter-day precision were < 6% and accuracy ranged between 96.9% and 99.8% for 25OHD3. Recovery was 84% and efficient control of matrix effects was verified. High sample throughput could be observed with 96 samples prepared in < 60 min. Close agreement of results was found for clinical samples analyzed with a second tandem mass spectrometry method based on protein precipitation and two-dimensional ultra-performance liquid chromatography for sample preparation (r = 0.988, n = 73). Conclusions: The new TICE™ technology was found to be a useful process for sample preparation in clinical mass spectrometry. Full automation suited for routine analysis was achieved.


Fang R.,Life Technologies | Liu J.Y.,Life Technologies | Kijenski H.L.,Life Technologies | Benfield J.,Life Technologies | And 9 more authors.
JALA - Journal of the Association for Laboratory Automation | Year: 2010

The HID EVOlution-qPCR/STR Setup System enables automation of DNA quantitative real-time polymerase chain reaction (PCR) setup, normalization of DNA sample, and PCR setup for short tandem repeat (STR) analysis. The HID EVOlution System tracks sample and reagent information and facilitates data transfer of DNA quantification, normalization, and PCR setup for STR analysis steps, eliminating the need for manual processing and repetitive data entry. Instruments for the automated system include a Tecan Freedom EVO 150 robot for liquid handling, the 7500 Real-Time PCR System for DNA quantification, the GeneAmp PCR System 9700 for STR amplification, and the 3130xl Genetic Analyzer for the detection of amplified STR fragments. Validation studies including reproducibility, accuracy, correlation, and contamination studies were performed. Results demonstrated clean liquid-handling capabilities and maintenance of sample integrity. Variation in average allele peak height obtained using automated protocol was similar to that obtained using the manual protocol. © 2010 The Association for Laboratory Automation.

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