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Durham, NC, United States

Zinn M.,Empa - Swiss Federal Laboratories for Materials Science and Technology | Durner R.,Tecan Group Ltd | Zinn H.,Alstom | Ren Q.,Empa - Swiss Federal Laboratories for Materials Science and Technology | And 3 more authors.
Biotechnology Journal | Year: 2011

It has been shown that Pseudomonas putida GPo1 is able to grow in continuous culture simultaneously limited by ammonium (N source) and octanoate (C source), and concomitantly accumulate poly([R]-3-hydroxyalkanoate) (PHA). Under such growth conditions the material properties of PHA can be fine-tuned if a second PHA precursor substrate is supplied. To determine the range of dual carbon and nitrogen (C, N)-limited growth conditions, tedious chemostat experiments need to be carried out for each carbon source separately. To determine the growth regime, the C/N ratio of the feed (f) to a chemostat was changed in a stepwise manner at a constant dilution rate of 0.3/h. Dual-(C, N)-limited growth was observed between C f/N f ≤ 6.4 g/g and C f/N f >9.5 g/g. In the following, we analyzed alternative approaches, using continuous medium gradients at the same dilution rate, that do not require time consuming establishments of steady states. Different dynamic approaches were selected in which the C f/N f ratio was changed continuously through a convex increase of C f, a convex increase of N f, or a linear decrease of C f (gradients 1, 2, and 3, respectively). In these experiments, the dual-(C, N)-limited growth regime was between 7.2 and 11.0 g/g ·for gradient 1, 4.3 and 6.9 g/g for gradient 2, and 5.1 and 8.9 g/g for gradient 3. A mathematical equation was developed that compensated a time delay of the gradient that was caused by the wash-in/wash-out effects of the medium feed. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Fu Y.V.,Harvard University | Yardimci H.,Harvard University | Long D.T.,Harvard University | Ho V.,State University of New York at Stony Brook | And 9 more authors.
Cell | Year: 2011

The eukaryotic replicative DNA helicase, CMG, unwinds DNA by an unknown mechanism. In some models, CMG encircles and translocates along one strand of DNA while excluding the other strand. In others, CMG encircles and translocates along duplex DNA. To distinguish between these models, replisomes were confronted with strand-specific DNA roadblocks in Xenopus egg extracts. An ssDNA translocase should stall at an obstruction on the translocation strand but not the excluded strand, whereas a dsDNA translocase should stall at obstructions on either strand. We found that replisomes bypass large roadblocks on the lagging strand template much more readily than on the leading strand template. Our results indicate that CMG is a 3′ to 5′ ssDNA translocase, consistent with unwinding via "steric exclusion." Given that MCM2-7 encircles dsDNA in G1, the data imply that formation of CMG in S phase involves remodeling of MCM2-7 from a dsDNA to a ssDNA binding mode. © 2011 Elsevier Inc.


Trademark
Tecan Group Ltd. | Date: 2014-03-11

Analytical apparatus and instruments for scientific or non-medical use, namely absorbance readers, luminescence readers, fluorescence readers, cell imaging instruments and array scanners; all the aforesaid products for laboratory use. Analytical apparatus and instruments for clinical diagnostic and veterinaric use, namely absorbance readers, luminescence readers, fluorescence readers, cell imaging instruments and array scanners; all the aforesaid products for laboratory use.


Trademark
Tecan Group Ltd. | Date: 2014-08-14

(Based on 44(d) foreign application) (Based on intent to use) Analysis apparatus, not for medical purposes, for analyzing presence, absence, or quantity of nucleic acids, antibodies, proteins or small molecules in material of biological origin; apparatus, not for medical purposes, comprising a control unit and a cassette for the analysis of biological substances for the presence, absence, or quantity of nucleic acids, antibodies, proteins or small molecules. (Based on 44(d) foreign application) (Based on intent to use) Apparatus for medical clinical analysis, namely, for analyzing presence, absence, or quantity of nucleic acids, antibodies, proteins or small molecules in samples of body fluids; apparatus, for medical clinical purposes, comprising a control unit and a cassette for the analysis of biological substances and samples for virus or bacterial infections diseases, gene mutations, hormone levels. (Based on 44(d) foreign application) (Based on intent to use) Industrial analysis in the field of chemistry and life sciences; scientific research; development of bio-analytical instruments for others; consultancy in the field of scientific, biochemical, biotechnological, biological, technological and industrial development; development, information and consultancy regarding scientific and technological analysis apparatus, diagnostic apparatus, and apparatus for the handling of micro volumes of liquids; development services and consultancy in the field of the technical handling of micro volumes of liquids and disposable cassettes for non-medical research diagnostics. (Based on intent to use) Consultancy in the field of medical diagnostics and in adapting medical diagnostic tests to micro volume scale tests.


Trademark
Tecan Group Ltd. | Date: 2014-03-11

Robots; automated systems used for picking up, setting down, transporting or positioning objects and solid, liquid or gaseous substances. Scientific, measuring, metering, optical, analytical and control apparatus and instruments, for laboratory requirements. Scientific, medical, optical, measuring, dosing and monitoring apparatus and instruments, for clinical diagnosis requirements.

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