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Ðà Lạt, Vietnam

Averyanov L.V.,RAS Komarov Botanical Institute | Van Duy N.,Tay Nguyen Institute of Biology | Loc P.K.,Hanoi University of Science and Technology
Taiwania | Year: 2012

Hymenorchis phitamii - new species for science discovered in southern Vietnam described and illustrated. From most closely related H. javanica it differs in emarginate orbicular lip and nearly straight (or hardly serrulate) tepals and leaves. The first record of the genus Hymenorchis in mainland Asia represents new remarkable addition to the orchid flora of Vietnam, as well as floras of Indochina and mainland Asia.

Nhut D.T.,Tay Nguyen Institute of Biology | Nam N.B.,Tay Nguyen Institute of Biology
IFMBE Proceedings | Year: 2010

Light-emitting diodes (LEDs) have been demonstrated to be a artificial flexible lighting source which has significant effects on biological processes. LEDs are not only the solution for biological studies but also for health caring projects. Numerous studies have been conducted in order to investigate the effects of LEDs on plants such as elongation, axillary shoot formation, leaf anatomy, and rhizogenesis as well as on animals such as cellular proliferation, collagen synthesis, growth factor metabolism in cells, cell growth enhancement, and cancer treatment. These studies have lead to many satisfactory results. The use of LEDs has a wide range of applications, such as a radiation source for plant production, investigations on animal nerve system and cell growth, and other applications for fishery and creative nature photographing, etc. © Springer-Verlag 2010.

Nhut D.T.,Tay Nguyen Institute of Biology | Hai N.T.,Tay Nguyen Institute of Biology | Thu P.T.M.,Tay Nguyen Institute of Biology | Thi N.N.,Tay Nguyen Institute of Biology | And 6 more authors.
Methods in Molecular Biology | Year: 2013

White or light purple flower color Torenia (Torenia fournieri Lind.) varieties were successfully developed from the parental variety having violet flowers. This was accomplished by reducing Fe micronutrient in the culture media for the induction of in vitro flowering. The flower induction was highest in modified Murashige and Skoog (MS) medium containing strength of macroelements, microelements, organic additives, and full Fe (M1) when compared to MS medium containing strength of macronutrients, micronutrients, full Fe, and full organic additives (M2). The flower color was stable in two new Torenia varieties through three generations ex vitro. The results showed a wide range of somaclonal variation in flower colors; early flowering occurred in MS medium containing strength of macroelements, microelements, Fe, and full strength of organic additives (M3). The selection of desirable somaclones and their micropropagation in subsequent generations led to the development of new and stable Torenia lines. © 2013 Springer Science+Business Media New York.

Nhut D.T.,Tay Nguyen Institute of Biology | Minh Thu H.T.,Tay Nguyen Institute of Biology | The Vinh B.V.,Ho Chi Minh City University of Technology | Van Binh N.,Tay Nguyen Institute of Biology | Quoc Luan V.,Tay Nguyen Institute of Biology
Propagation of Ornamental Plants | Year: 2012

In this study, the morphogenesis of Cyclamen persicum Mill. using the thin cell layer technology (TCL) was successfully established. The peduncles were sliced into thin cell layers (1 mm thick), which were then cultured on MS medium supplemented with NAA, IBA, 2,4-D at various concentrations (0.1; 0.3; 0.5; 0.7 or 1.0 mg l-1) alone or in combination with TDZ (0.2 mg l-1). The results showed that the presence of auxin alone in variants of culture medium was not sufficient for callus induction from peduncle TCL explants. MS medium supplemented with 0.2 mg l-1 TDZ and 1.0 mg l-1 2,4-D was found to be the optimal medium for callus induction. Different sizes of thin cell layer explants (0.5, 1.0, 2.0 or 3.0 mm) from two positions of the peduncle (the upper or lower position of peduncle) were also studied. The 3 mm initiating explants at the upper position of the peduncle gave 100% callus induction rate and high callus fresh weight. After 8 weeks of culture, calluses which were transferred onto MS medium supplemented with 0.5 mg l-1 BA and 0.7 mg l-1 IBA induced the highest number of adventitious shoots (39.4 shoots/explant). Rooting reached 100% after transferring 2-3 cm long shoots to MS medium supplemented with 1.0 mg l-1 IBA. The results indicated that different kinds of plant growth regulators, the explant size and position are important in determining the morphogenesis from peduncle TCL explants of Cyclamen persicum Mill.

Duong T.N.,Tay Nguyen Institute of Biology | Nguyen T.H.,Tay Nguyen Institute of Biology | Mai X.P.,Tay Nguyen Institute of Biology
Methods in molecular biology (Clifton, N.J.) | Year: 2010

A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 +/- 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.

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