Taurida Academy Of Vi Vernadsky Crimean Federal University

Simferopol, Russia

Taurida Academy Of Vi Vernadsky Crimean Federal University

Simferopol, Russia
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Oberemok V.V.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Laikova K.V.,Medical Academy Of Vi Vernadsky Crimean Federal University | Zaitsev A.S.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Nyadar P.M.,Taurida Academy Of Vi Vernadsky Crimean Federal University | And 4 more authors.
Journal of Plant Protection Research | Year: 2016

The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV-infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by th antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant protection against insect pests. © 2017 by Palmah M. Nyadar.


Mutah N.P.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Adesoji A.T.,Taurida Academy Of Vi Vernadsky Crimean Federal University
International Journal of Pest Management | Year: 2017

In this study, we designed and analysed the insecticidal effectiveness of two short single-stranded DNA fragments from LdMNPV (Lymantria dispar multicapsid nuclear polyhedrosis virus) IAP-2 gene. Our results show that the ssDNA fragments from the antisense region is selectively lethal, and rapidly decreases the population of second instar gypsy moth (Lymantria dispar L.) larvae one day post-treatment (Control-H2O group vs oligoAn group, χ2 = 5; p < 0.05) when infected with LdMNPV as a trigger, before treatment through feeding with 75 pmol/µl of synthesized fragments from LdMNPV IAP-2 gene. Data analysis on larvae biomass did not show significant influence in all groups of the experiment when compared with the Control-H2O group. Relatively, our preliminary evaluation of biochemical parameters (total glucose and total protein concentrations) did not show significant response to the applied ssDNA oligonucleotides. Our conclusive remarks based on mortality data support the concept that antisense oligonucleotides from LdMNPV IAP-2 gene can act as an insecticide against pre-infected gypsy moth larvae. © 2017 Informa UK Limited, trading as Taylor & Francis Group


Zaitsev A.S.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Omel'chenko O.V.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Nyadar P.M.,Taurida Academy Of Vi Vernadsky Crimean Federal University | Oberemok V.V.,Taurida Academy Of Vi Vernadsky Crimean Federal University
Bulletin of the Transilvania University of Brasov, Series II: Forestry, Wood Industry, Agricultural Food Engineering | Year: 2015

In this paper we evaluated the impact of the DNA oligonucleotides used as insecticides on the biochemical parameters of English oak (Quercus robur) and apple seedlings (Malus domestica). The assessment of the biochemical parameters of the plants was based on the measurement of the alkaline phosphatase activity and glucose concentration, important markers in plant cell, in the leaves of English oak and apple seedlings. We have found that the use of the RING domain fragment in a concentration of 5 pmol/cm2 of plant leaf, leads to a significant decrease in alkaline phosphatase activity in apple seedlings (p<0.05) and to a significant decrease in the concentration of glucose in the leaves of English oak (p<0.01) compared to the control, 24 hours after the treatment. However, the values of the parameters in the experimental groups were non-significant, compared to the control on the 7th day. These results suggest the absence of a long-term negative effect of the DNA oligonucleotides on apple seedlings and leaves of English oak.

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