Time filter

Source Type

Kamble R.D.,Tatyasaheb Kore Institute of Engineering and Technology | Jadhav A.R.,Smt. K.R.P. Kanya Mahavidyalaya
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: The aim of this experimental study was production, purification and characterization of alkali stable xylanase from locally isolated Cellulosimicrobium sp. MTCC 10645, which is an important industrial enzyme used in the pulp and paper industry. Methods: The enzyme was produced in Erlenmeyer flasks containing fresh basal salt medium supplemented with 1% oat spelt xylan. The enzyme was extracted and isolated using ammonium sulphate precipitation and dialysis. It was further purified using DEAE cellulose chromatography and purity was checked by SDS-PAGE. Effect of temperature and pH on activity and stability of enzyme was studied. The enzyme was laso studied for its substrate specificity and kinetic parameters. Results: The isolate was identified on the basis of cultural, morphological, physiological and biochemical properties as well as 16S rRNA sequencing. Among the carbon sources tested, birchwood xylan found prominent for increased level of xylanase i. e. 96.33 U/ml. The enzyme was purified by DEAE cellulose chromatography at NaCl concentration of 0.25 M and had a molecular mass of 78.0 kDa. Xylanase was purified sixteen fold with a specific activity of 246.6 U/mg. Xylanase activity was maximum at 50°C. The enzyme was thermostable retaining 8% of the original activity after incubation at 60°C of 4 h. The enzyme was active over a pH range of 6.0-11.0, although its activity was optimal at pH 7.0. About 48.52% of the enzyme activity was retained after 4 h at pH 11.0. The enzyme was active on oat spelt and birchwood xylans but not on avicel, CMC, cellobiose, starch or p-nitrophenyl xylopyranoside. The xylanase had Km and Vmax values of 4.76 mg/ml and 232.5 μmol/min/mg, respectively when birchwood xylan used as substrate. Conclusions: The xylanase showed a unique pattern of xylan hydrolysis releasing a large amount of intermediate products (xylotriose and xylobiose) with small quantity of xylose. Some of these characteristics make this enzyme potentially effective in xylan biodegradation and pulp bleaching. © 2012 Asian Pacific Tropical Biomedical Magazine.


Desai S.S.,Tatyasaheb Kore Institute of Engineering and Technology
New Mathematics and Natural Computation | Year: 2016

The aim of this paper is to study a quasi-transitive rationality of the fuzzy choice functions through indicators. In this paper, we introduce the indicators of the path independent property, fuzzy Condorcet property and fuzzy α2 condition of a fuzzy choice function. These indicators measure the degree to which the fuzzy choice function satisfies the fuzzy path independent, fuzzy Condorcet property and fuzzy α2 condition, respectively. We express the indicator of quasi-transitive rationality in terms of the indicator of the path independent, Condorcet property and fuzzy α2 condition. © 2016 World Scientific Publishing Company.


Karjinni V.V.,Tatyasaheb Kore Institute of Engineering and Technology | Anadinni S.B.,Sai Vidya Institute of Technology
Journal of Structural Engineering (Madras) | Year: 2010

In this experimental work, a mix design for Self-Compacting Concrete (SCC) has been carried out following Nan-Su method.1 The flow properties such as passing ability, filling ability, and segregation resistance2 were evaluated by conducting various tests by varying the dosage of super plasticizer and viscosity modifying admixture (VMA) with fly ash, silica fume and metakaolin as mineral admixtures. Following Nan-Su method for the mix proportions of SCC for M20, M30, M40 and M50 were arrived. Mechanical properties viz. compressive strength, split tensile strength and flexural strength were carried out on cubes, cylinders and beam specimen, respectively. It has been observed from the test results; the quantity of cement was found to be insufficient to get the required strength for all the above said grades and some trials have been made to increase the cement quantity by reducing the mineral admixtures to arrive required strength for normal grade of SCC. The comparative studies have been made on compressive strength, tensile strength, flexural strength, young's modulus, Poisson's ratio and density of concrete and are determined after 7 and 28 days curing. Comparing the mechanical properties with conventional concrete laid out some conclusions. Also an attempt has been made to incorporate some suggestions in the Nan-Su method for the normal grades (M20 to M50) of Self-Compacting Concrete.


Desai S.S.,Tatyasaheb Kore Institute of Engineering and Technology | Chaudhari S.R.,North Maharashtra University
Fundamenta Informaticae | Year: 2015

Many researchers have studied the rationality of fuzzy choice functions with transitive rationalization. However, not much is known when transitivity is weakened to quasi-transitivity or any other weaker property of the preference relation. In the present paper we study the rationality of fuzzy choice functions with quasi-transitive rationalization for the domains that contain characteristic functions of all single and two element subsets of the universal set. © 2015 IOS Press and the authors. All rights reserved.


Desai S.,Tatyasaheb Kore Institute of Engineering and Technology
New Mathematics and Natural Computation | Year: 2014

In literature many researchers have studied full rationality of fuzzy choice functions. The aim of the present paper is to discuss rationality of fuzzy choice functions with rationalization reflexive, complete and quasi-transitive. For this purpose, we use fuzzy path independent property and fuzzy Condorcet property. We characterize rationality of fuzzy choice functions with reflexive, complete and quasi-transitive rationalization in terms of these axioms. © 2014 World Scientific Publishing Company.


Vambase S.V.,Tatyasaheb Kore Institute of Engineering and Technology | Mangalwede S.R.,Gogte Institute of Technology
Proceedings of 2014 International Conference on Contemporary Computing and Informatics, IC3I 2014 | Year: 2014

One of the most widely used resources is electricity. However, electrical network has a serious issue of uniform distribution of electricity. This paper investigates the distributed generation systems in electrical network and proposes an interdisciplinary mechanism for effective sharing of electricity across the electrical sub-network. The proposed mechanism is based on wireless mesh network and micro-controller. The proposed system forms a wireless mesh network used to exchange information (such as current status, predicted status of electricity generators) between mesh nodes (in WMN). The information from mesh node is used by micro-controller to drive the electrical devices. The micro-controller is responsible for controlling the direction of flow of electricity and quantity of electricity to be permitted on a particular part of electrical network. © 2014 IEEE.


Sharma A.K.,Shivaji University | Patil A.V.,Tatyasaheb Kore Institute of Engineering and Technology | Haridas R.N.,Shivaji University
Exploration Geophysics | Year: 2012

Electromagnetic anomalies covering a wide range of frequencies from ultra low frequency (ULF), very low frequency (VLF) up to very high frequency (VHF) have been observed before earthquakes. However, the ULF range emissions provide a greater source of information regarding the earthquake precursor. One of the main techniques of investigating such a precursor is by using a magnetic sensor. In this paper, we have carried out a study of spectral density (magnetic field intensity) and polarization ratio methods to extract earthquake precursory signatures of the ULF data for moderate earthquakes (magnitude M b=3.74.8), using a three-component induction coil magnetometer installed at Shivaji University, Kolhapur (16.40°N, 74.15°E), India. We have applied a Fast Fourier Transform (FFT) procedure to calculate the spectral density of the ULF time series. We have found enhancement in ULF magnetic field intensity 3 to 5 days before the main shock and this specific enhancement appeared ±3h around the main shock time in the 15Hz frequency range. We have examined ULF variations with polarization values and K p index data. Magnetic field intensity of ULF data can give important information about earthquake preparation processes and it can be involved in the development of earthquake prediction methodology. © 2012 ASEG.


Desai S.,Tatyasaheb Kore Institute of Engineering and Technology | Potdar R.,Veermata Jijabai Technological Institute
New Mathematics and Natural Computation | Year: 2016

This paper introduces indicators of the weak fuzzy T-congruence axiom and the fuzzy Chernoff axiom. These indicators measure the degree to which the fuzzy choice function satisfies the weak fuzzy T-congruence axiom and the fuzzy Chernoff axiom. The indicator of the full rationality is expressed in terms of indicators of weak fuzzy T-congruence axiom and the fuzzy Chernoff axiom. © 2016 World Scientific Publishing Company.


Kulkarni R.B.,Gogte Institute of Technology | Vaghe V.M.,Tatyasaheb Kore Institute of Engineering and Technology
International Journal of Advanced Structural Engineering | Year: 2014

Cold-formed structural members are being used more widely in routine structural design as the world steel industry moves from the production of hot-rolled section and plate to coil and strip, often with galvanized and/or painted coatings. Steel in this form is more easily delivered from the steel mill to the manufacturing plant where it is usually cold-rolled into open and closed section members. In the present experimental study, the use of packing plate at the joints in cold-formed channel sections may increase the load carrying capacity and also reduce the buckling of unconnected cold form channel steel plate at joints. The present study focuses on examining the experimental investigation to use mild steel as a packing plate with cold-formed channel sections by bolted connection at the joints and the connection subjected to axial tension. Series of tests are carried out with increase in the thickness of packing plate and results are observed and analyzed. Total Twelve experimental tests have been carried out on cold-formed channel tension members fastened with single as well as three numbers of bolts at the connection and from the observations the strength of the joint is increased by increasing the various thicknesses of packing plates and also the buckling of unconnected leg of channel specimen is reduced. It is analyzed by plotting the entire load versus elongation path, so that the behavior of the connection is examined. © 2014, The Author(s).


Kamble R.D.,Tatyasaheb Kore Institute of Engineering and Technology | Jadhav A.R.,K.R.P. Kanya Mahavidyalaya
International Journal of Microbiology | Year: 2012

A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was 29.8kDa. The optimum temperature and pH for the enzyme activity were 50C and pH 8.0. The enzyme was active on birchwood xylan and little active on p-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity. For birchwood xylan, the enzyme gave a Km 5.26mg/mL and Vmax 277.7mol/min/mg, respectively. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry. © 2012 Rajashri D. Kamble and Anandrao R. Jadhav.

Loading Tatyasaheb Kore Institute of Engineering and Technology collaborators
Loading Tatyasaheb Kore Institute of Engineering and Technology collaborators