Kamble R.D.,Tatyasaheb Kore Institute of Engineering and Technology |
Jadhav A.R.,Smt KRP Kanya Mahavidyalaya
Asian Pacific Journal of Tropical Biomedicine | Year: 2012
Objective: The aim of this experimental study was production, purification and characterization of alkali stable xylanase from locally isolated Cellulosimicrobium sp. MTCC 10645, which is an important industrial enzyme used in the pulp and paper industry. Methods: The enzyme was produced in Erlenmeyer flasks containing fresh basal salt medium supplemented with 1% oat spelt xylan. The enzyme was extracted and isolated using ammonium sulphate precipitation and dialysis. It was further purified using DEAE cellulose chromatography and purity was checked by SDS-PAGE. Effect of temperature and pH on activity and stability of enzyme was studied. The enzyme was laso studied for its substrate specificity and kinetic parameters. Results: The isolate was identified on the basis of cultural, morphological, physiological and biochemical properties as well as 16S rRNA sequencing. Among the carbon sources tested, birchwood xylan found prominent for increased level of xylanase i. e. 96.33 U/ml. The enzyme was purified by DEAE cellulose chromatography at NaCl concentration of 0.25 M and had a molecular mass of 78.0 kDa. Xylanase was purified sixteen fold with a specific activity of 246.6 U/mg. Xylanase activity was maximum at 50°C. The enzyme was thermostable retaining 8% of the original activity after incubation at 60°C of 4 h. The enzyme was active over a pH range of 6.0-11.0, although its activity was optimal at pH 7.0. About 48.52% of the enzyme activity was retained after 4 h at pH 11.0. The enzyme was active on oat spelt and birchwood xylans but not on avicel, CMC, cellobiose, starch or p-nitrophenyl xylopyranoside. The xylanase had Km and Vmax values of 4.76 mg/ml and 232.5 μmol/min/mg, respectively when birchwood xylan used as substrate. Conclusions: The xylanase showed a unique pattern of xylan hydrolysis releasing a large amount of intermediate products (xylotriose and xylobiose) with small quantity of xylose. Some of these characteristics make this enzyme potentially effective in xylan biodegradation and pulp bleaching. © 2012 Asian Pacific Tropical Biomedical Magazine.
Desai S.,Tatyasaheb Kore Institute of Engineering and Technology
New Mathematics and Natural Computation | Year: 2014
In literature many researchers have studied full rationality of fuzzy choice functions. The aim of the present paper is to discuss rationality of fuzzy choice functions with rationalization reflexive, complete and quasi-transitive. For this purpose, we use fuzzy path independent property and fuzzy Condorcet property. We characterize rationality of fuzzy choice functions with reflexive, complete and quasi-transitive rationalization in terms of these axioms. © 2014 World Scientific Publishing Company.
Vambase S.V.,Tatyasaheb Kore Institute of Engineering and Technology |
Mangalwede S.R.,Gogte Institute of Technology
Proceedings of 2014 International Conference on Contemporary Computing and Informatics, IC3I 2014 | Year: 2014
One of the most widely used resources is electricity. However, electrical network has a serious issue of uniform distribution of electricity. This paper investigates the distributed generation systems in electrical network and proposes an interdisciplinary mechanism for effective sharing of electricity across the electrical sub-network. The proposed mechanism is based on wireless mesh network and micro-controller. The proposed system forms a wireless mesh network used to exchange information (such as current status, predicted status of electricity generators) between mesh nodes (in WMN). The information from mesh node is used by micro-controller to drive the electrical devices. The micro-controller is responsible for controlling the direction of flow of electricity and quantity of electricity to be permitted on a particular part of electrical network. © 2014 IEEE.
Sharma A.K.,Shivaji University |
Patil A.V.,Tatyasaheb Kore Institute of Engineering and Technology |
Haridas R.N.,Shivaji University
Exploration Geophysics | Year: 2012
Electromagnetic anomalies covering a wide range of frequencies from ultra low frequency (ULF), very low frequency (VLF) up to very high frequency (VHF) have been observed before earthquakes. However, the ULF range emissions provide a greater source of information regarding the earthquake precursor. One of the main techniques of investigating such a precursor is by using a magnetic sensor. In this paper, we have carried out a study of spectral density (magnetic field intensity) and polarization ratio methods to extract earthquake precursory signatures of the ULF data for moderate earthquakes (magnitude M b=3.74.8), using a three-component induction coil magnetometer installed at Shivaji University, Kolhapur (16.40°N, 74.15°E), India. We have applied a Fast Fourier Transform (FFT) procedure to calculate the spectral density of the ULF time series. We have found enhancement in ULF magnetic field intensity 3 to 5 days before the main shock and this specific enhancement appeared ±3h around the main shock time in the 15Hz frequency range. We have examined ULF variations with polarization values and K p index data. Magnetic field intensity of ULF data can give important information about earthquake preparation processes and it can be involved in the development of earthquake prediction methodology. © 2012 ASEG.
Kulkarni R.B.,Gogte Institute of Technology |
Vaghe V.M.,Tatyasaheb Kore Institute of Engineering and Technology
International Journal of Advanced Structural Engineering | Year: 2014
Cold-formed structural members are being used more widely in routine structural design as the world steel industry moves from the production of hot-rolled section and plate to coil and strip, often with galvanized and/or painted coatings. Steel in this form is more easily delivered from the steel mill to the manufacturing plant where it is usually cold-rolled into open and closed section members. In the present experimental study, the use of packing plate at the joints in cold-formed channel sections may increase the load carrying capacity and also reduce the buckling of unconnected cold form channel steel plate at joints. The present study focuses on examining the experimental investigation to use mild steel as a packing plate with cold-formed channel sections by bolted connection at the joints and the connection subjected to axial tension. Series of tests are carried out with increase in the thickness of packing plate and results are observed and analyzed. Total Twelve experimental tests have been carried out on cold-formed channel tension members fastened with single as well as three numbers of bolts at the connection and from the observations the strength of the joint is increased by increasing the various thicknesses of packing plates and also the buckling of unconnected leg of channel specimen is reduced. It is analyzed by plotting the entire load versus elongation path, so that the behavior of the connection is examined. © 2014, The Author(s).