Tatar Agriculture Research Institute

Kazan, Russia

Tatar Agriculture Research Institute

Kazan, Russia
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Hadieva G.F.,Kazan Federal University | Karamova N.S.,Kazan Federal University | Stasevski Z.,Tatar Agriculture Research Institute | Djabbarova E.M.,Kazan Federal University | And 2 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016

Fusarium species are the most frequently fungal pathogens of potato worldwide. In this study we isolated Fusarium species causing dry rot in potatoes that were grown in Republic of Tatarstan in 2014. Isolated species were identified as Fusarium oxysporum (4 strains), Fusarium solani (1), Fusarium avenaceum (1), Fusarium tricinctum (1), Fusarium sambucinum (1) and Fusarium redolens(1) by sequencing the ITS regions of rRNA. Variable degrees of pathogenicity were observed with the collected Fusarium isolates after artificial inoculation of healthy potato tubers. Fusarium oxysporum strains (MG2, NK3, MG1) were the most aggressive and virulent followed by Fusariumsolani NZ1, Fusarium tricinctum SA1and Fusarium sambucinum NK2, Fusarium avenaceum NK1 were the least pathogenic. The results of the identification of prevalent pathogenic Fusarium isolates from potato tubers can contribute to development of regional strategies for controlling the disease development in this area.


Kuzminova O.A.,Kazan Federal University | Vologin S.G.,Tatar Agriculture Research Institute | Stasevski Z.,Tatar Agriculture Research Institute | Gimaeva E.A.,Tatar Agriculture Research Institute | And 2 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016

We studied the resistance to potato virus Y (PVY) in a potato hybrid population, and its effect on the yield formation under contrasting meteorological conditions. The virus resistance analysis was conducted after growing the plants under natural virus infection and after PVY inoculation. The dominant Ryadg gene allele in the potato breeding lines genotypes was revealed by detection of the PCR-based molecular marker RYSC3. Virus resistant lines were selected more often in the potato breeding program than susceptible forms due to higher productivity and larger number of tubers. PVY-resistant samples demonstrated a higher yield than susceptible forms. Significant differences (p < 0.05) were detected by univariate ANOVA in three of the four years studied. The virus resistance effect value ranged from 4 to 22%. Resistant form yield was more stable than that of susceptible one, which was evident from the corresponding coefficients of variation. Resistant samples also had a greater number of tubers per plant. At the same time, univariate ANOVA revealed significant differences (p < 0.05) only in one of the four studied years. The virus resistance effect ranged from 2 to 22%.


Vafin R.R.,Tatar Agriculture Research Institute | Tyulkin S.V.,Bauman Kazan State Academy of Veterinary Medicine | Zagidullin L.R.,Bauman Kazan State Academy of Veterinary Medicine | Muratova A.V.,Bauman Kazan State Academy of Veterinary Medicine | And 6 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016

The main purpose of the research was to develop and prove effective PCR methods for cattle genotyping by allelic variants of the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene. The article describes two PCR methods for genotyping of cattle by alleles A and K of gene DGAT1: the first method based on electrophoretic detection, which comprises using two forward allele-specific primers and one reverse primer; and the second method based on real-time hybridization fluorescence detection, which uses two 5/-fluorescently labeled forward allele-specific primers, one non-modified reverse primer, and one anti-primer labeled with fluorescence extinguisher at 3/-end of oligonucleotide. The approved PCR methods have correctly identified the analyzed genotypes, showing consistent results, comparable to the estimated data and to the results of preliminary research studies based on PCR-RFLP of the same cattle sampling.


Tikhonova O.Y.,Kazan Federal University | Toymentseva A.A.,Kazan Federal University | Savenkova A.A.,Tatar Agriculture Research Institute | Gizatullina A.T.,Tatar Agriculture Research Institute | And 4 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

In this study effects of genotype, explants size and the composition of the culture medium on regeneration capacity of apexes of etiolated potato tubers sprouts were estimated. We observed positive correlation among survival, growth and development of plant tissues in vitro and the size of plant explants. When the cultivation medium was supplemented by RNase A (1-10 μg/ml) and ribonucleases from Bacillus pumilus (RNAse Bp, 1 μg/ml) 35% increase of the number of the regenerants was observed. Enzymes with ribonuclease activity at low concentrations (1-10 μg/ml) stimulated regenerative and morphogenic processes. In contrast neither plant regeneration dynamic nor plant morphogenesis were changed when cultivation medium was supplemented by other Bacillus pumilus proteolytic enzymes (subtilisin-like protease (AprBp), glutamyl endopeptidase (GseBp) and metalloendopeptidase (MprBp) in concentration of 1 μg/ml.

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