Vafin R.R.,Tatar Agriculture Research Institute |
Tyulkin S.V.,Bauman Kazan State Academy of Veterinary Medicine |
Zagidullin L.R.,Bauman Kazan State Academy of Veterinary Medicine |
Muratova A.V.,Bauman Kazan State Academy of Veterinary Medicine |
And 6 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016
The main purpose of the research was to develop and prove effective PCR methods for cattle genotyping by allelic variants of the Diacylglycerol O-Acyltransferase 1 (DGAT1) gene. The article describes two PCR methods for genotyping of cattle by alleles A and K of gene DGAT1: the first method based on electrophoretic detection, which comprises using two forward allele-specific primers and one reverse primer; and the second method based on real-time hybridization fluorescence detection, which uses two 5/-fluorescently labeled forward allele-specific primers, one non-modified reverse primer, and one anti-primer labeled with fluorescence extinguisher at 3/-end of oligonucleotide. The approved PCR methods have correctly identified the analyzed genotypes, showing consistent results, comparable to the estimated data and to the results of preliminary research studies based on PCR-RFLP of the same cattle sampling.
Tikhonova O.Y.,Kazan Federal University |
Toymentseva A.A.,Kazan Federal University |
Savenkova A.A.,Tatar Agriculture Research Institute |
Gizatullina A.T.,Tatar Agriculture Research Institute |
And 4 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015
In this study effects of genotype, explants size and the composition of the culture medium on regeneration capacity of apexes of etiolated potato tubers sprouts were estimated. We observed positive correlation among survival, growth and development of plant tissues in vitro and the size of plant explants. When the cultivation medium was supplemented by RNase A (1-10 μg/ml) and ribonucleases from Bacillus pumilus (RNAse Bp, 1 μg/ml) 35% increase of the number of the regenerants was observed. Enzymes with ribonuclease activity at low concentrations (1-10 μg/ml) stimulated regenerative and morphogenic processes. In contrast neither plant regeneration dynamic nor plant morphogenesis were changed when cultivation medium was supplemented by other Bacillus pumilus proteolytic enzymes (subtilisin-like protease (AprBp), glutamyl endopeptidase (GseBp) and metalloendopeptidase (MprBp) in concentration of 1 μg/ml.