Bowman J.P.,Tasmania Institute of Agriculture
Comprehensive Analytical Chemistry | Year: 2014
Proteomics involves analysis of the complement of proteins in a system. In recent years, the transition from a gel-based approach to proteomics has been rapidly advanced to include gel-free, label-free, quantitative procedures that offer both deep analysis of proteomes and absolute determination of cell protein concentrations. This review covers these developments and relates the applicability of such approaches to the study of prokaryotic systems including the current state-of-the-art procedures as well as more basic aspects such as protein extraction, protein fractionation, methods for protein quantification, and visualization of data in relation to functional ontology. Proteomics is proving to be in a period of rapid technological development and represents a leading aspect of the vanguard in the postgenomic era, providing a valuable knowledge component within the science systems biology. © 2014 Elsevier B.V.
Tarbath M.P.,Tasmania Institute of Agriculture |
Measham P.F.,Tasmania Institute of Agriculture |
Glen M.,Tasmania Institute of Agriculture |
Barry K.M.,Tasmania Institute of Agriculture
Australasian Plant Pathology | Year: 2014
Fungal rot of sweet cherry fruit leads to reduced yield and quality at harvest. This study investigated a range of host factors related to cherry rot and also identified the main species present in latently infected-fruit and fruit with visible rot at harvest in a Tasmanian orchard. There was a significant effect of tree cultivar on total rot found at harvest, with the cultivar “Sweetheart” associated with almost double (approx. 25 % infection) the amount of rotten fruit compared to “Regina” and “Simone” (approx. 13 %). Harvest date may have had some influence on this result, as Sweetheart fruit were harvested 2 weeks later than the other cultivars, and there was a large rainfall event just before Sweetheart harvest (rainfall can increase disease spread and infection). Crop load did not influence the amount of rotten fruit found at harvest for any cultivar, although crop load was low in this season at this site. Removing fungicide application from the point of 50 % full bloom onwards did not significantly alter disease incidence. The study found that Botrytis cinerea was the dominant rot pathogen in cherry fruit at this site and season. No evidence of Monilinia species was found in this study with the methods used, despite it being typically associated with rot of stone fruit. Fruit quality characteristics were not indicators of disease susceptibility, as very few characteristics observed to correlate with disease at harvest. © 2014, Australasian Plant Pathology Society Inc.