Tasly Jenner Biotechnology Tianjin Co.

Tianjin, China

Tasly Jenner Biotechnology Tianjin Co.

Tianjin, China

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Tian W.-L.,Tasly Jenner Biotechnology Tianjin Co. | Yang J.-S.,Tasly Jenner Biotechnology Tianjin Co. | Wan Y.-F.,Tasly Jenner Biotechnology Tianjin Co. | Qi J.,Tasly Jenner Biotechnology Tianjin Co. | And 2 more authors.
Chinese Journal of Biologicals | Year: 2012

Objective: To determine the formaldehyde content in influenza virus subunit vaccine by acetylacetone spectrophotometry. Methods: The formaldehyde content in influenza virus subunit vaccine was determined by acetylacetone spectrophotometry, based on which the detection wavelength, concentrations of acetylacetone, acetic acid and ammonium acetate as well as time for maintaining and lowering the temperature were optimized, and the method was verified. Results: The optimal reaction system consisted of 500 μl of test sample and 4.5 ml of acetylacetone. The optimal concentrations of acetylacetone, acetic acid and ammonium acetate were 0.125%, 1.5% and 25% respectively, while the optimal time for maintaining (at 40°C) and lowering the temperature were both 30 min, and the optimal detection wavelength was 414 nm. Under the optimized condition, the determination result was satisfactory. The standard curve showed good linearity within a concentration range of 1-50 μg/ml, while the recovery rate and minimum detection limit were 101.9% and 1 μg/ml respectively. Conclusion: Acetylacetone spectrophotometry was simple, rapid, accurate and sensitive for determination of formaldehyde content in influenza virus subunit vaccine, which was suitable for the routine determination of formaldehyde content in laboratory.


Tian W.-L.,Tasly Jenner Bio Technology Tianjin Co. | Yang J.-S.,Tasly Jenner Bio Technology Tianjin Co. | Song Q.-Y.,Tasly Jenner Bio Technology Tianjin Co. | Wan Y.-F.,Tasly Jenner Bio Technology Tianjin Co. | And 3 more authors.
Chinese Journal of Biologicals | Year: 2011

Objective: To develop a HPLC method for detection of cleavage reagent nonoxynol-9 content in influenza virus subunit vaccine. Methods: The test samples of influenza virus subunit vaccine were precipitated with four volumes of methanol. The condition for HPLC was as follows: column: Thermo BioBasic-4 C 4 column (4.6 mm x 150 mm, 5 μm); mobile phase: methanol : water = 80:20 (v/v); flow rate: 1 ml/min; detection wavelength of ultraviolet detector: 275 nm; column temperature: 33°C; sample load: 100 μl. Results: The developed HPLC method showed good linearity for detection of nonoxynol-9 at concentrations of 5-2500 μg/ml. The method showed also high specificity for nonoxynol-9. The recovery rates of nonoxynol-9 in inactivated influenza whole virus vaccine extract and in influenza virus subunit vaccine were both about 100%. Both intra- and inter-precision of determination results of nonoxynol-9 standard and influenza virus subunit vaccine were less than 3%. Both the limits of detection and quantification were 5 μg/ml. Conclusion: The developed HPLC method was simple, rapid, accurate and suitable for the routine laboratory determination of cleavage reagent content in influenza virus subunit vaccine.


Tian W.-L.,Tasly Jenner Bio Technology Tianjin Co. | Yang J.-S.,Tasly Jenner Bio Technology Tianjin Co. | Wan Y.-F.,Tasly Jenner Bio Technology Tianjin Co. | Qi J.,Tasly Jenner Bio Technology Tianjin Co. | Yang X.,Tasly Jenner Bio Technology Tianjin Co.
Chinese Journal of Biologicals | Year: 2013

Objective: To compare the determination results of content of nonoxynol-9 as a cleavage reagent in influenza virus subunit vaccine by HPLC and UV spectrophotometry. Methods: The nonoxynol-9 contents in intermediate and final product of influenza virus subunit vaccine were determined by HPLC and UV spectrophotometry separately, and the intermediate was analyzed by SDS-PAGE. Results: The nonoxynol-9 contents in intermediate and final product, especially in intermediate, of influenza virus subunit vaccine determined by UV spectrophotometry increased by more than 10% as compared with those by HPLC. SDS-PAGE showed the nucleoprotein (NP) contents were significantly different in intermediates 1, 2 and 3, which was the highest in intermediate 3. Conclusion: HPLC may be used for determination of nonoxynol-9 content in influenza virus subunit vaccine instead of UV spectrophotometry.

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