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Jin L.,Nanjing Agricultural University | Zhao W.-S.,Tasly Pharmaceutical Group Co. | Guo Q.-S.,Nanjing Agricultural University | Zhang W.-S.,Tasly Pharmaceutical Group Co. | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To establish a UPLC fingerprint method of Paeoniae Alba Radix, and provide comprehensive evaluation of their quality in different regions. Methods: The UPLC chromatographic column was Acquity UPLC® HSS T3 (100 mm × 2.1 mm, 1.8 μm). The mobile phase was acetonitrile-0.05% phosphoric acid water with gradient elution. The detection wavelength was 230 nm and column temperature was 30 ℃ with the flow rate of 0.4 mL/min. Similarity analysis, hierarchical clustering analysis, and principal component analysis were undertaken to study 23 sets of UPLC fingerprints of Paeoniae Alba Radix. Results: A specific UPLC fingerprint of Paeoniae Alba Radix was established and eight common peaks were designated. The results showed that the qualities of the 23 sets of Paeoniae Alba Radix were not stable and the samples collected from same region and different regions both had certain differences. Conclusion: UPLC fingerprint is an available and convenient method which can be used to access the quality of Paeoniae Alba Radix rapidly. © 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Han M.,Tasly Holding Group Co. | Han M.,Tianjin Medical University | Wang G.-C.,Tasly Holding Group Co. | Duan H.-Q.,Tianjin Medical University
Chinese Chemical Letters | Year: 2014

The self-assembly of cationic perylene diimide (PDI) and anionic cholesterol derivatives (CHOL) was conveniently achieved by the electrostatic attraction and π-π stacking interactions, exhibiting the well-defined supramolecular nanofibers ranging from hundreds of nanometers to micron dimension. © 2013 Guo-Cheng Wang and Hong-Quan Duan.

Chu Y.,Tasly Academy | Yan K.-J.,Tasly Academy | Li W.,Tasly Academy | Ma X.-H.,Tasly Academy | And 5 more authors.
Chinese Journal of New Drugs | Year: 2015

Objective: To investigate the pharmacokinetics and bioequivalence of domestic and imported temozolomide capsules in Beagle dogs. Methods: In a two-way crossover trial, six Beagle dogs randomly received a single crossover oral dose of domestic (test preparation) and imported (reference preparation) temozolomide capsules. The concentration of temozolomide in dog plasma was determined by HPLC. By using Bapp 3.0 software, the pharmacokinetic parameters were calculated and the bioequivalence of the two preparations was evaluated. Results: The main pharmacokinetic parameters of the test and reference capsules were as follows: Cmax were (11.35±1.35) and (11.70±2.77) μg·mL-1; Tmax were (0.97±0.56) and (1.08±0.49) h; t1/2β were (1.44±0.37) and (1.43±0.24) h; AUC0~t were (27.34±2.30) and (28.34±7.47) μg·h·mL-1, AUC0~∞ were (29.85±4.49) and (30.85±8.46) μg·h·mL-1, respectively. The relative bioavailability of the test capsules was (106.1±36.1)%. Conclusion: The test and reference temozolomide capsules meet the regulatory criteria for the pharmacokinetic equivalence, thus are bioequivalent in Beagle dogs. ©, 2015, Chinese Journal of New Drugs Co. Ltd. All right reserved.

Du X.,Zhejiang Sci-Tech University | Zhang C.,Shaanxi University of Technology | Guo W.,Zhejiang Sci-Tech University | Jin W.,Zhejiang Sci-Tech University | And 6 more authors.
Molecules | Year: 2015

Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-D-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (Nω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation. © 2015 by the authors; licensee MDPI.

Xing B.,Zhejiang Sci-Tech University | Yang D.,Zhejiang Sci-Tech University | Guo W.,Zhejiang Sci-Tech University | Liang Z.,Zhejiang Sci-Tech University | And 3 more authors.
Molecules | Year: 2015

Phenolic acids and tanshinones are two groups of bioactive ingredients in Salvia miltiorrhiza Bunge. As a heavy metal elicitor, it has been reported that Ag+ can induce accumulations of both phenolic acids and tanshinones in S. miltiorrhiza hairy roots. In this study, the effects of Ag+ treatment on accumulations of six phenolic acids and four tanshinones in S. miltiorrhiza hairy roots were investigated. To further elucidate the molecular mechanism, expressions of key genes involved in the biosynthesis of these ingredients were also detected. The results showed that although the total phenolic acids content was almost not affected by Ag+ , accumulations of rosmarinic acid (RA), caffeic acid and ferulic acid were significantly increased, while accumulations of salvianolic acid B (LAB), danshensu (DSU) and cinnamic acid were decreased. We speculate that LAB probably derived from the branch pathway of DSU biosynthesis. Contents of four tanshinones were enhanced by Ag+ and their accumulations were more sensitive to Ag+ than phenolic acids. Genes in the upstream biosynthetic pathways of these ingredients responded to Ag+ earlier than those in the downstream biosynthetic pathways. Ag+ probably induced the whole pathways, upregulated gene expressions from the upstream pathways to the downstream pathways, and finally resulted in the enhancement of ingredient production. Compared with phenolic acids, tanshinone production was more sensitive to Ag+ treatments. This study will help us understand how secondary metabolism in S. miltiorrhiza responds to elicitors and provide a reference for the improvement of the production of targeted compounds in the near future. © 2014 by the authors licensee MDPI Basel Switzerland.

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