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Chandrasekar A.,Tamil Nadu Veterinary and Animal Sciences University | Raja A.,Tamil Nadu Veterinary and Animal Sciences University | Dhinakar Raj G.,Translational Research Platform for Veterinary Biologicals | Thangavelu A.,Madras Veterinary College | Kumanan K.,TANUVAS
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2015

A reverse-transcription loop mediated isothermal amplification (RT-LAMP) was developed for rapid diagnosis of infectious bronchitis (IB) in poultry by targeting the spike protein 2 gene (S2). RT-LAMP primers were designed for IBV-S2 targets and optimized to run at 60 °C for 45 min. As compared with RT-PCR, RT-LAMP was 100 times more sensitive for IBV-S2 gene. RT-LAMP showed specific amplification with IB viral genome but not with other avian respiratory pathogens due to their mismatching with IBV-S2-RT-LAMP primers. RT-LAMP reaction products were visually detected by the addition of propidium iodide stain. Out of 102 field samples tested for detection of IBV, RT-LAMP detected IBV in 12 samples for S2 gene whereas RT-PCR detected IBV in six samples for S2 gene. The sensitivity of the RT-LAMP was 100 % and the specificity was 94 % for S2 gene. Since the developed RT-LAMP to detect IBV is simple, rapid, sensitive and specific, it can be a useful diagnostic tool for detection of IB in poultry in less equipped laboratories and in field conditions. © 2015, The National Academy of Sciences, India.

Jerome A.,Indian Veterinary Research Institute | Sarath T.,Indian Veterinary Research Institute | Arunmozhi N.,TANUVAS
Buffalo Bulletin | Year: 2010

A conjoined twin monster fetus was delivered by per-vaginum in a pluriparous buffalo, which was presented with dystocia. The twin monster consisted of two female fetuses which were fully developed having eight limbs, i.e. four forelimbs and four hind limbs, joined together at the anterior part of the body. The monster can be classified as conjoined twin monster (Thoraco abdominopygophagus).

Manjunath S.,Madras Veterinary College | Vijayarani K.,Madras Veterinary College | Kumanan K.,TANUVAS
Indian Journal of Animal Sciences | Year: 2014

The present study was carried out to clone and express fusion protein gene of Newcastle disease virus in mammalian expression system. The Newcastle disease virus (2K3) isolate from pigeon was propagated in 9-11 day-old embryonated chicken eggs and infected allantoic fluid was collected for RNA isolation. The fusion (F) gene of 1.68 Kb was amplified with cDNA as template and was cloned in TOPO cloning vector. The recombinant TOPO colonies were digested with NcoI and XhoI enzymes, the insert released was further ligated into pTriEx Neo 1.1 expression vector digested with the same enzymes and was transformed in Escherichia coli. The recombinant pTriEx colonies confirmed by colony PCR and restriction digestion were induced with 1mM IPTG which showed expressed fusion protein of 55 kDa at 4 h post induction and increased in overnight induced cultures. The recombinant pTriEx plasmid was transfected into vero cells. The cell lysate collected at 48 h and 72 h post transfection showed expressed fusion protein with the molecular weight of 55 kDa in 12% SDS-PAGE. The protein was further confirmed to be NDV specific by its immunoreactivity with NDV antiserum raised in rabbits showing fusion protein of 55 kDa. The immunofluorescence assay of transfected vero cells exhibited a bright cytoplasmic fluorescence confirming the fusion protein expression.

Jeichitra V.,TANUVAS | Jeichitra V.,Madras Veterinary College | Rajendran R.,TANUVAS | Karunanithi K.,TANUVAS | And 2 more authors.
Indian Journal of Animal Research | Year: 2016

Data on 1763 Mecheri sheep, maintained at the Mecheri Sheep Research Station, Pottaneri, Salem, south India, and recorded between 1991 and 2006, were analysed to study the growth related traits and their genetic control. The average weights at birth and at 12 months of age were 2.25 ± 0.01 and 17.48 ± 0.14 kg, respectively. The pre- and post-weaning (3-6, 3-9 and 3-12) average daily weight gains were 63.40 ± 0.58, 39.57 ± 0.57, 37.48 ± 0.44 and 34.31 ± 0.42 g respectively. The heritabilities of body weights and weight gains were in general moderate to high. The phenotypic and genetic correlations among body weights were positive and moderate to high. The phenotypic and genetic correlations among average daily gains were positive and low to high. The estimates of genetic correlation among average daily gains and body weights were positive and high with few exceptions. © 2016, Agricultural Research Communication Centre. All rights reserved.

Prabakar G.,Indian Veterinary Research Institute | Gopi M.,ICAR Central Avian Research Institute | Karthik K.,TANUVAS | Shanmuganathan S.,Indian Veterinary Research Institute | And 2 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2016

The ever increasing consumer’s awareness and their concern over the presence of antibiotic residues in poultry products necessitate looking for an alternative to antibiotic growth promoters. Among the numerous alternatives like probiotics, prebiotics, acidifiers, the plant origin compound attracts more interest than else. The phytochemicals consists of various natural products that may be nutritional, non-nutritional or anti-nutritional in nature. These phyto-chemicals also act as an antibacterial, antiviral, antifungal, antioxidant, digestive stimulant, immune-modulator, hypo-lipidemic agent and also heat stress alleviator. A compound of multi-functional can be considered as additive in animal production system. Their anti-oxidant activity and hypo-lipidemic property of these plant derived compounds will be attributed for the improvement of shelf-life of various animal or poultry products. Their hypolipidemic properties are used in production of lean meat production. These phytobiotics also impart readily acceptable flavour to the products especially meat and eggs. They aid in digestive process by stimulating the digestive secretions throughout the gastro-intestinal tract thereby increasing the overall digestibility of the nutrients and reduce the environmental pollution. Moreover, these botanical products are becoming more prominent in insect and pest control strategies due to their availability and cost. With their wide range of activities, these phytobiotics will go to be a new group of feed additives for better growth rather than simply as another alternate to in-feed antibiotics in food production industry. © 2016 Govinthasamy Prabakar et al.

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