Kurayoshi, Japan
Kurayoshi, Japan

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Sugimoto H.,Taniguchi Hospital | Sugimoto H.,Kinki University | Kida Y.,Kinki University | Oh N.,Kinki University | And 5 more authors.
Zygote | Year: 2015

We examined growing oocytes collected from follicles remaining in superovulated rabbit ovaries, that were grown (in vitro growth, IVG) and matured (in vitro maturation, IVM) in vitro. We produced somatic cell nuclear transfer (SCNT) embryos using the mature oocytes and examined whether these embryos have the ability to develop to the blastocyst stage. In addition, we examined the effects of trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), on the developmental competence of SCNT embryos derived from IVG-IVM oocytes. After growth for 7 days and maturation for 14-16 h in vitro, the growing oocytes reached the metaphase II stage (51.4%). After SCNT, these reconstructed embryos reached the blastocyst stage (20%). Furthermore, the rate of development to the blastocyst stage and the number of cells in the blastocysts in SCNT embryos derived from IVG-IVM oocytes were significantly higher for TSA-treated embryos compared with TSA-untreated embryos (40.6 versus 21.4% and 353.1 ± 59.1 versus 202.5 ± 54.6, P < 0.05). These results indicate that rabbit SCNT embryos using IVG-IVM oocytes have the developmental competence to reach the blastocyst stage. Copyright © Cambridge University Press 2014.


Sugimoto H.,Taniguchi Hospital | Kida Y.,Kinki University | Miyamoto Y.,Kinki University | Kitada K.,Kitayama Labes Co | And 4 more authors.
Theriogenology | Year: 2012

The objective was to develop a culture system that produced blastocyst stage embryos from rabbit oocytes grown in vitro. Two experiments were performed. First, various concentrations of fetal bovine serum (FBS, 0, 0.05, 0.5 and 5%) were used in the culture medium for in vitro growth (IVG) of oocytes recovered from follicles 200 to 299 μm in diameter. Intracytoplasmic sperm injection (ICSI) was performed on mature oocytes obtained after IVG for 8 days and in vitro maturation for 14 to 16 h. Rates of survival and pronuclear formation after ICSI were higher for oocytes grown in a medium with 0.05% FBS compared to oocytes grown in a medium lacking FBS (97.6 vs. 76.9%, 97.5 vs. 70%, P < 0.1). The rate of development to the blastocyst stage was also higher in the medium containing 0.05% FBS than in the medium lacking FBS (9.5 vs. 17.9%, P < 0.05). Next, using oocytes recovered from follicles 200 to 399 μm in diameter which were cultured in 0.05% FBS, oxygen consumption and the number of cells were analyzed. Blastocysts from oocytes grown in vitro with 0.05% FBS had reduced oxygen consumption and number of cells compared with those from ovulated oocytes (21.66 ± 4.54 × 1014 vs. 50.19 ± 4.61 × 1014 mol/sec, 244 ± 25 vs. 398 ± 24, P < 0.05). Rabbit oocytes grown in vitro with 0.05% FBS achieved pregnancy, but pregnancies were not maintained to term. In conclusion, the addition of 0.05% FBS to the culture medium for IVG improved developmental competence of rabbit oocytes grown in vitro. © 2012 Elsevier Inc.


Sawa R.,Kobe University | Doi T.,Center for Gerontology and Social Science | Doi T.,Japan Society for the Promotion of Science | Asai T.,Kobe Gakuin University | And 3 more authors.
Gait and Posture | Year: 2015

The aim of this study was to compare gait characteristics, including the functional ability of the trunk, between women before and during the third trimester of pregnancy. Gait measurements were performed on 27 pregnant women, who were divided into two groups using the threshold of 28 gestational weeks. The subjects were instructed to walk at their preferred speed. In addition to stride-time coefficient of variation, root mean square (RMS) and autocorrelation coefficient, coefficient of attenuation (CoA) of acceleration was computed as an index to assess the functional ability of the trunk. Differences of gait characteristics between the groups were determined by the Mann-Whitney U test. Gait characteristics that showed a significant difference between the groups were further analyzed with adjustment by age, height, weight and gait velocity by using multiple regression analysis. Women during the third trimester of pregnancy showed significantly smaller RMS in the anteroposterior direction at the lower trunk than those before the third trimester of pregnancy, even after adjusting for age, height, weight and gait velocity [ β= 0.47; 95% confidence interval (CI) 0.07-0.25]. CoA in the anteroposterior direction was also significantly lower in women during the third trimester of pregnancy than in those before the third trimester of pregnancy after adjustment by age, height, weight and gait velocity (β. = 0.44; 95% CI 0.39-18.52). The present cross-sectional study suggests the possibility that the functional ability of the trunk during gait declines in late pregnancy. © 2015 Elsevier B.V.


Miyagawa I.,Tottori University | Ohata R.,Red Cross | Sasaki N.,Red Cross | Koumi T.,Red Cross | And 7 more authors.
Nishinihon Journal of Urology | Year: 2011

Nocturia causes sleep disturbance, which has been associated with impairment of daytime activity and QOL (quality of life). Deep sleep should appear by three hours after bedtime. It is thought that an adequate duration of sleep until the 1st voiding after bedtime is important for health, and contributes towards maintaining a patient's good QOL. The present study investigated whether the a 1-adrenoceptor blocker, naftopidil, prolongs the duration of sleep. Naftopidil was administered to patients with nocturia associated with benign prostatic hyperplasia for a period of 12 weeks. Nighttime frequency and duration of sleep until the 1st voiding after bedtime were evaluated by a micturition time chart, and international prostate symptom score (IPSS) and QOL score were also assessed at 12 weeks after administration. After treatment by naftopidil for 12 weeks in 15 patients, nighttime frequency was decreased significantly from 2.9 ± 1.5 to 2.1 ± 1.1 times (p < 0.01) and duration of sleep until the 1st voiding after bedtime was significantly prolonged from 114.7 ± 56.6 to 157.7 ± 66.5 minutes (p < 0.05). Furthermore naftopidil significantly improved IPSS (from 18.3 ± 6.7 to 12.1 ± 7.4, p < 0.01) and QOL score (from 4.5 ± 1.2 to 2.9 ± 1.2, p < 0.01). Naftopidil seems to improve both nocturia and duration of sleep until the 1st voiding after bedtime, which should improve not only the quality of sleep but also daytime activity.


PubMed | Japan Society for the Promotion of Science, Kobe Gakuin University, Taniguchi Hospital, Kobe University and University of Shiga Prefecture
Type: Journal Article | Journal: Gait & posture | Year: 2015

The aim of this study was to compare gait characteristics, including the functional ability of the trunk, between women before and during the third trimester of pregnancy. Gait measurements were performed on 27 pregnant women, who were divided into two groups using the threshold of 28 gestational weeks. The subjects were instructed to walk at their preferred speed. In addition to stride-time coefficient of variation, root mean square (RMS) and autocorrelation coefficient, coefficient of attenuation (CoA) of acceleration was computed as an index to assess the functional ability of the trunk. Differences of gait characteristics between the groups were determined by the Mann-Whitney U test. Gait characteristics that showed a significant difference between the groups were further analyzed with adjustment by age, height, weight and gait velocity by using multiple regression analysis. Women during the third trimester of pregnancy showed significantly smaller RMS in the anteroposterior direction at the lower trunk than those before the third trimester of pregnancy, even after adjusting for age, height, weight and gait velocity [=0.47; 95% confidence interval (CI) 0.07-0.25]. CoA in the anteroposterior direction was also significantly lower in women during the third trimester of pregnancy than in those before the third trimester of pregnancy after adjustment by age, height, weight and gait velocity (=0.44; 95% CI 0.39-18.52). The present cross-sectional study suggests the possibility that the functional ability of the trunk during gait declines in late pregnancy.


Miyoshi R.,University of Miyazaki | Matsuo H.,University of Miyazaki | Naono-Nagatomo K.,University of Miyazaki | Ozono K.,Taniguchi Hospital | And 5 more authors.
Asian Journal of Psychiatry | Year: 2014

Aim: This study examined whether daily self-monitoring of weight and monthly interviews with a doctor improved eating habits and led to weight loss, and whether temperament and character traits affect weight change in persons with schizophrenia. Methods: Participants used Sakata's Charting of Daily Weight Pattern to monitor their weight daily. In addition, Sakata's Eating Behavior Questionnaire was administered to evaluate eating-behavior awareness. The Temperament and Character Inventory (TCI) was used to assess participants' temperament and character. Fifty patients were divided into two groups: the intervention group (n= 25) filled in Sakata's Charting of Daily Weight Pattern every day; was interviewed monthly by a doctor about weight management; was weighed monthly. The non-intervention group (n= 25) was only weighed monthly. Results: The body mass index (mean±standard error: 0.59±0.10kg/m2, p<0.001) of the intervention group decreased significantly while their scores on Sakata's Eating Behavior Questionnaire significantly improved albeit marginally. Conversely, body mass index increased significantly (0.66±0.18kg/m2, p<0.001) in the non-intervention group, whose scores on Sakata's Eating Behavior Questionnaire did not change significantly. Weight change and TCI scores were not correlated for the intervention group, but scores for "self-directedness" and weight gain in the non-intervention group had a marginally significant negative correlation (r=-0.33, p<0.10). Conclusion: Our results suggest that monitoring one's weight daily on Sakata's Charting of Daily Weight Pattern led to improvements in eating behavior and a decrease in BMI of patients with schizophrenia. © 2013 Elsevier B.V.


PubMed | University of Miyazaki and Taniguchi Hospital
Type: Journal Article | Journal: Asian journal of psychiatry | Year: 2014

This study examined whether daily self-monitoring of weight and monthly interviews with a doctor improved eating habits and led to weight loss, and whether temperament and character traits affect weight change in persons with schizophrenia.Participants used Sakatas Charting of Daily Weight Pattern to monitor their weight daily. In addition, Sakatas Eating Behavior Questionnaire was administered to evaluate eating-behavior awareness. The Temperament and Character Inventory (TCI) was used to assess participants temperament and character. Fifty patients were divided into two groups: the intervention group (n = 25) filled in Sakatas Charting of Daily Weight Pattern every day; was interviewed monthly by a doctor about weight management; was weighed monthly. The non-intervention group (n = 25) was only weighed monthly.The body mass index (mean standard error: 0.59 0.10 kg/m(2), p < 0.001) of the intervention group decreased significantly while their scores on Sakatas Eating Behavior Questionnaire significantly improved albeit marginally. Conversely, body mass index increased significantly (0.66 0.18 kg/m(2), p < 0.001) in the non-intervention group, whose scores on Sakatas Eating Behavior Questionnaire did not change significantly. Weight change and TCI scores were not correlated for the intervention group, but scores for self-directedness and weight gain in the non-intervention group had a marginally significant negative correlation (r = -0.33, p < 0.10).Our results suggest that monitoring ones weight daily on Sakatas Charting of Daily Weight Pattern led to improvements in eating behavior and a decrease in BMI of patients with schizophrenia.


Fujime M.,Osaka University | Tomimatsu T.,Osaka University | Okaue Y.,Taniguchi Hospital | Koyama S.,Osaka University | And 3 more authors.
Hypertension Research | Year: 2012

The current study tested the hypothesis that pregnancy-related changes are more pronounced in central hemodynamics, and both central aortic systolic blood pressure (cSBP) and augmentation index (AIx) are independent from brachial systolic blood pressure (bSBP) in normal pregnant subjects. In 830 healthy pregnant women from 12 to 36 weeks gestation, we measured cSBP and AIx-75 (AIx at heart rate of 75 beats per minute) non-invasively by pulse waveforms of the radial artery using an automated applanation tonometric system. In 69 pregnant women, we recorded these data longitudinally. cSBP and AIx-75 significantly declined during pregnancy, reaching its nadir in mid-pregnancy and rising towards term. Pregnancy-related changes were more pronounced in AIx-75 compared with cSBP, but less evident in bSBP. AIx-75, but not cSBP, was independent from bSBP throughout pregnancy. cSBP and AIx-75, but not bSBP, were significantly increased in healthy pregnant women older than 35 years. This study established normal values for pulse wave analysis parameters throughout pregnancy, and indicated that pulse wave analysis might offer additional and independent information about maternal arterial compliance to conventional brachial blood pressure measurements. These data may be used as the basis for further investigation into the role of pulse wave analysis in the assessment, management and prediction of disorders, which might interfere with pregnancy-related cardiovascular adaptations. © 2012 The Japanese Society of Hypertension All rights reserved.


Suzuki F.,Taniguchi Hospital
Nihon rinsho. Japanese journal of clinical medicine | Year: 2013

Smoking causes various health problems in women in relation to their life cycle. About the effects of smoking on obstetric and gynecological disorders, it is clarified that smoking has adverse effects on menopausal disorders, miscarriage, premature birth, low birth weight infant, breast cancer, uterine cancer and ovarian cancer. The high rate of smoking among women of reproductive age is of particular concern for the next generation because smoking affects not only the women themselves, but also the fetus. It is necessary to promote smoking prevention education to prevent women from developing a smoking habit and to provide smoking cessation education and support for smokers.


PubMed | Taniguchi Hospital
Type: Journal Article | Journal: Theriogenology | Year: 2012

The objective was to develop a culture system that produced blastocyst stage embryos from rabbit oocytes grown in vitro. Two experiments were performed. First, various concentrations of fetal bovine serum (FBS, 0, 0.05, 0.5 and 5%) were used in the culture medium for in vitro growth (IVG) of oocytes recovered from follicles 200 to 299 m in diameter. Intracytoplasmic sperm injection (ICSI) was performed on mature oocytes obtained after IVG for 8 days and in vitro maturation for 14 to 16 h. Rates of survival and pronuclear formation after ICSI were higher for oocytes grown in a medium with 0.05% FBS compared to oocytes grown in a medium lacking FBS (97.6 vs. 76.9%, 97.5 vs. 70%, P < 0.1). The rate of development to the blastocyst stage was also higher in the medium containing 0.05% FBS than in the medium lacking FBS (9.5 vs. 17.9%, P < 0.05). Next, using oocytes recovered from follicles 200 to 399 m in diameter which were cultured in 0.05% FBS, oxygen consumption and the number of cells were analyzed. Blastocysts from oocytes grown in vitro with 0.05% FBS had reduced oxygen consumption and number of cells compared with those from ovulated oocytes (21.66 4.54 10(14) vs. 50.19 4.61 10(14) mol/sec, 244 25 vs. 398 24, P < 0.05). Rabbit oocytes grown in vitro with 0.05% FBS achieved pregnancy, but pregnancies were not maintained to term. In conclusion, the addition of 0.05% FBS to the culture medium for IVG improved developmental competence of rabbit oocytes grown in vitro.

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