Wang J.-W.,Tangshan Worker Hospital |
Liu Y.,Peking Union Medical College |
Tian H.-M.,Peking Union Medical College |
Zhang W.,Peking Union Medical College
Chinese Journal of Cancer Research | Year: 2010
Objective: Survivin is one of the apoptosis inhibitor genes and is rarely expressed in adulttissues. However, survivin expression has been detected in various human cancers and correlations have been recognized between the level of expression of this gene in tumors and prognosis. In this study, we investigated the effect of Survivin-siRNA on the drug sensitivity of osteosarcoma cell line MG-63. Methods: Two siRNAs (Survivin-siRNA1, Survivin-siRNA2) specifically targeting Survivin gene were chemically synthesized and transfected into MG-63 cells. The Survivin mRNA level was detected by reverse transcription-polymerase chain reaction (RT-PCR). The survivin protein expression and cell apoptosis rate were analyzed by flow cytometry (FCM). The 50% inhibition concentration (IC50) of cisplatin (DDP) and adriamycin (ADM) on MG-63 cells was determined by MTT method. Results: Two short siRNA targeting survivin down-regulated the transcription of survivin gene dramatically and elevated apoptosis rate. They increased the drug sensitivity of MG-63 cells to ADM by five-fold and to DDP by nine-fold. Conclusion: Validated Survivin specific siRNA can effectively inhibit Survivin expression in survivin-overexpressing osteosarcoma MG-63 cell line and enhance the drug sensitivity of MG-63 cell line to ADM and DDP. Short survivin-siRNA mediated gene silencing may be a useful therapeutic strategy for osteosarcoma. These results suggest that survivin might be helpful for diagnosis of osteosarcoma and survivin siRNA combined with adriamycin or cisplatin may be a feasible strategy to enhance the effects of chemotherapy in patients with osteosarcoma. © 2010 Chinese Anti-Cancer Association and Springer Berlin Heidelberg.
Wang D.-C.,Capital Medical University |
Zhang Y.,Nanchang University |
Chen H.-Y.,University of Utah |
Li X.-L.,Hebei United University |
And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012
Gliomas are a group of heterogeneous primary central nervous system tumors. Hyperthermia has proven to be a potential therapeutic tool for cancers in the clinic. However, the molecular mechanisms of hyperthermia remain unclear. The objective of this study was to investigate the effects of hyperthermia on the invasiveness in C6 glioma cells and related molecular pathways. Here our data show hyperthermia stimulated the release of tumor necrosis factor-alpha. (TNF-α) and decreased C6 glioma cell migration and invasive capability at 30, 60, 120 and 180 min; with increased spontaneous apoptosis in C6 glioma cells at 120 min. We also found mitogenactivated protein kinase. (P38 MAPK) protein expression to be increased and nuclear factor-kappa B. (NF-κB) protein expression decreased. Based on the results, we conclude that hyperthermia alone reduced invasion of C6 glioma cells through stimulating TNF-α signaling to activate apoptosis, enhancing P38 MAPK expression and inhibiting the NF-κB pathway, a first report in C6 rat glioma cells.
Wang D.,Tangshan Worker Hospital |
Zhang Y.,Jiangsu University |
Li X.,Tangshan Worker Hospital |
Cui J.,Tangshan Worker Hospital |
Wang S.,Capital Medical University
Medical Oncology | Year: 2012
Tumor-associated glycoprotein-72 (TAG-72) is a high molecular weight tumor-associated glycoprotein, which is known to be overexpressed in various human tumors, but its expression in glioma tissues is unknown. Therefore, the aim of this study was to investigate whether TAG-72 is present in glioma and to evaluate the correlation between TAG-72 expression and the severity of the malignancy of this tumor. Immunohistochemistry and Western blot were used to investigate the expression of TAG-72 protein, respectively, in 152 patients with gliomas. There were 90 men and 62 women (mean age 50.6 ± 11.8 years). Astrocytoma was found in 130 patients and glioblastoma in 22. No TAG-72 expression was found in the non-cancerous brain tissues. TAG-72 protein expression was identified in 80 patients with glioma (52.6%). The expression level of TAG-72 protein was increased from gliomas with low grades to those with high grades. The highest mean value of TAG-72 protein was found in patients with glioblastoma (P < 0.01), whereas the lowest mean values were found in those with grade I astrocytoma (P < 0.01). Our results provide convincing evidence for the first time that the expression of TAG-72 is up-regulated in human gliomas. The expression levels of TAG-72 in gliomas were associated with the severity of the tumor. Whether positive TAG-72 protein expression can be used as a predictor for prognosis of the patients or as a therapeutic target for glioma requires further investigation. Copyright © Springer Science+Business Media, LLC 2011.
Chen L.-L.,Tangshan Worker Hospital |
Huang L.-M.,Tangshan Worker Hospital |
Zhan H.-Y.,Tangshan Worker Hospital |
Cao Y.-B.,Tangshan Worker Hospital
Chinese Journal of New Drugs | Year: 2011
Objective: To observe the expression of C-fos protein in the brain at different stages after pilocarpine-induced seizure. Methods: Epileptic seizure was induced by pilocarpine in rats. The behavioral changes were observed, and the expression of C-fos in the brain in different stages of seizure was detected by immunohistochemistry. Results: The C-fos expression in hippocampal CA1, CA3, dentate gyrus and cerebral cortex was significantly different at different stages of seizure (P < 0.05). In the early stages of seizure (stage I and II), C-fos was mainly expressed in the cortex. With increasing of the degree of convulsion, C-fos expression expanded in the dentate gyrus, CA1, CA3 and cortex in stages IV and V. Conclusion: In the early stages of epileptic seizure, C-fos begins to express, so in order to decrease neurons damage measurements should be taken to early monitor.
Wang L.-M.,Tangshan Worker Hospital |
Wang X.-Y.,Tangshan Worker Hospital |
Zhao Y.-C.,Tangshan Worker Hospital |
Peng P.,Tangshan Worker Hospital |
Zhu C.-Q.,Tangshan Worker Hospital
World Chinese Journal of Digestology | Year: 2016
AIM: To explore the method of decreasing liver cyst recurrence after laparoscopic decompression. METHODS: The clinical and postoperative follow-up data for 72 patients who underwent liver cyst surgery at Tangshan Worker Hospital were analyzed retrospectively. Efficacy of open surgery and laparoscopic liver cyst drainage for treatment of liver cysts was compared. RESULTS: Compared with open surgery, laparoscopic liver cyst drainage had the advantages of less pain and shorter hospitalization time. However, the liver cyst recurrence rate was 5/24 (20.1%) after drainage, which was higher than 3/48 (6.3%) after open surgery. Sodium hyaluronate could effectively reduce cyst recurrence after laparoscopic liver cyst drainage by preventing postoperative liver wound adhesions. CONCLUSION: Laparoscopic liver cyst drainage is safe and feasible. Unroofing of the liver cyst as much as possible, using sclerosing agents rationally to inactivate residual cyst epithelial cells, and effective drainage on the premise of proper use of sodium hyaluronate and other anti-adhesion agents could effectively reduce cyst recurrence after laparoscopic surgery. © 2016 Baishideng Publishing Group Inc.