Kumar V.,Tamilnadu Fisheries University |
Sukumar D.,Tamilnadu Fisheries University |
Muruganantham M.,Tamilnadu Fisheries University
Indian Journal of Animal Research | Year: 2015
Squids perish rapidly like other fishes and require some treatment to maintain the quality for export. The microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with the commercial food grade chemicals imported from Spain was therefore studied. Dressed squids were treated with the chemicals Hidratech_4A (0.4%) and Whitech_3 (0.25%) dissolved in chilled freshwater (STCF) and chilled saltwater (STCS). Chilled squids not treated served as control (SNTC). Squids were quick frozen at -40ºC in contact plate freezer and stored at-20±1ºC. Samples were tested raw, after pre-processing, treatments and freezing and during storage at monthly intervals for 7 months. The microbial quality evaluation included Total Plate Count (TPC), Escherichia coli, Staphylococcus aureus, Vibrio cholerae and Salmonella. Study revealed a better quality of treated samples than control. Microbiological quality of STCS was better than STCF and SNTC. E. coli counts decreased after treatment. Salmonella and V. cholerae were absent. © 2015, Agricultural Research Communication Centre. All rights reserved.
Kowsalya R.,Dr. M.G.R. Educational and Research Institute |
Karrunakaran C.M.,Dr. M.G.R. Educational and Research Institute |
Deecaraman M.,Dr. M.G.R. Educational and Research Institute |
Uma A.,Tamilnadu Fisheries University |
Singh S.,Chandigarh Institute of Microbial Technology
International Journal of Pharma and Bio Sciences | Year: 2013
The effect of textile effluents in Cyprinus carpio (Common carp) was studied by experimental exposure to effluents at three different levels (10%, 15% and 20% v/v).The histopathological changes in the gills of common carp was recorded at day 1, 2, 4 and 7 after exposure to textile effluents. Results showed that textile effluents causes histopathological changes in the gills of Cyprinus carpio which included congestion, fusion of gill lamellae and degeneration of secondary gill epithelium.
PubMed | Tamilnadu Fisheries University
Type: Journal Article | Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] | Year: 2015
A study was performed to investigate the genomic variations in the shrimp farm isolates of Vibrio alginolyticus and V. harveyi when the isolates were subjected to environmental stress. Samples of shrimps, water and sediment were collected from Southern Indian coastal shrimp farms. Vibrio isolates were biochemically identified and confirmed using 16S rDNA and gyrB gene specific PCR. The bacterial strains were genotyped by PCR fingerprinting using GTG(5) and IS (Insertion Sequence) primers. Seven strains each of V. alginolyticus and V. harveyi were subjected to 10 passages through trypticase soya broth (TSB), which contained different NaCl concentrations (3, 6 and 8%) and trypticase soya agar (TSA). V. alginolyticus was also passaged through TSB with a 12% NaCl concentration. PCR fingerprinting, which was performed on the strains that were passaged through different salt concentrations, confirmed that V. alginolyticus and V. harveyi could affect the genomic variations, depending on the environmental conditions of the culture. The study highlights the complex genotypic variations that occur in Vibrio strains of tropical aquatic environment because of varied environmental conditions, which result in genetic divergence and/or probable convergence. Such genetic divergence and/or convergence can lead to the organismal adaptive variation, which results in their ability to cause a productive infection in aquatic organisms or generation of new strains.