Tamil Nadu Veterinary and Animal science University is the first veterinary university in Asia, founded in 1989 in Madhavaram Milk Colony, Chennai, India. It is composed of the Madras Veterinary College, Veterinary College and Research Institute, Namakkal and the Fisheries College and Research Institute in Thoothukkudi and the Institute of Food and Dairy Technology, Koduvalli, Chennai-52. Research farms are for leprosy bacteria, for prawn and edible fish culture, and for animal feed safety. Wikipedia.
Eswari S.,Tamil Nadu Veterinary and Animal Sciences University |
Sai Kumar G.,Indian Veterinary Research Institute |
Sharma G.T.,Indian Veterinary Research Institute
Zygote | Year: 2013
Summary The objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus-oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo semen. After 18 h of co-incubation with sperm, the presumptive zygotes were cultured in modified synthetic oviductal fluid without (control) or with rhLIF (100 ng/ml). There was no significant difference in the overall cleavage rate up to morula stage however the development of blastocysts, hatching rate and total cell numbers were significantly higher in the LIF-treated group than control. Transcripts for LIFRβ were detected from immature, in vitro-matured oocytes and in the embryos up to blastocyst stage, while transcripts for the LIF were detected from 8-16-cell stage up to blastocyst, which indicated that embryo-derived LIF can act in an autocrine manner on differentiation process and blastocyst formation. This study indicated that the addition of LIF to the embryo culture medium improved development of blastocysts, functional (hatching) and morphological (number of cells) quality of the blastocysts produced in vitro. The stage-specific expression pattern of LIF and LIFRβ mRNA transcripts in buffalo embryos indicated that LIF might play an important role in the preimplantation development and subsequent implantation of buffalo embryos. © Cambridge University Press 2012.
Chaudhry U.,University of Calgary |
Redman E.M.,University of Calgary |
Raman M.,Tamil Nadu Veterinary and Animal Sciences University |
Gilleard J.S.,University of Calgary
International Journal for Parasitology | Year: 2015
It is important to understand how anthelmintic drug resistance mutations arise and spread in order to determine appropriate mitigation strategies. We hypothesised that a molecular genetic study of Haemonchus contortus in southern India, a region where resistance may be less advanced than in western Europe and North America, might provide some important insights into the origin and spread of anthelmintic resistance. The F200Y (T. AC) isotype-1 β-tubulin benzimidazole resistance mutation is common in H. contortus throughout the world and the F167Y (T. AC) and E198A (G. CA) mutations, although less common, have been reported in a number of different countries. We have investigated the haplotypic diversity and phylogenetic relationship of isotype-1 β-tubulin benzimidazole resistance alleles for 23 H. contortus populations from small ruminants across southern India. The F200Y (T. AC) mutation was most common, being detected in 18/23 populations at frequencies between 9% and 84% and the E198A (G. CA) mutation was also detected in 8/23 populations at frequencies between 8% and 18%. The F167Y (T. AC) mutation was not detected in any of the 23 populations. Phylogenetic haplotype network analysis suggested that the F200Y (T. AC) mutation has arisen multiple independent times in the region with at least three independent origins of resistance alleles across the populations surveyed. In contrast, the E198A (G. CA) mutation was present on a single haplotype which, given the high level of haplotypic diversity of the susceptible alleles in the region, suggests this particular mutation has spread from a single origin, likely by anthropogenic animal movement. Population genetic analysis of 12 of the H. contortus populations, using a panel of eight microsatellite markers, revealed extremely low genetic differentiation between populations, consistent with the hypothesis of high gene flow among sites. Additionally, there was no significant genetic differentiation between H. contortus taken from sheep and goats which is consistent with H. contortus populations being freely shared between these two different hosts. Overall, we believe these results provide the first clear genetic evidence for the spread of an anthelmintic resistance mutation to multiple different locations from a single origin. © 2015.
Anandh M.A.,Tamil Nadu Veterinary and Animal Sciences University
Journal of Applied Animal Research | Year: 2014
Cooked restructured buffalo meat rolls prepared from restructuring process were stored at 4 ± 1°C in polyethylene terephthalate laminated with polythene pouches under vacuum packaging condition. The samples were evaluated for physicochemical parameters, microbial quality and sensory attributes at regular intervals of 0, 5, 10, 15, 25 and 30 days of storage. A significant decrease in pH, extract release volume values, was observed with increasing storage period. Overall days mean for moisture decreased with increasing storage period, however, no significant difference in overall days mean for moisture content was observed on from day 0 to day 30 of storage. A significant (P < 0.01) increase in thiobarbituric acid value and tyrosine value was observed with increase in storage period. Microbiological counts increased with the advancement of storage period. However, throughout the storage period, all microbial counts were within the acceptable limits of cooked meat products. Boiled restructured buffalo meat rolls did not show any symptoms of spoilage such as off odour and surface slime on day 30 of storage and were acceptable for sensory quality up to 30 days of refrigerated storage (4 ± 1°C) under vacuum packaging. Thus, the present study indicates that vacuum packaging could be used as a way to improve the shelf life of boiled restructured buffalo meat rolls without affecting the physicochemical, microbiological and sensory qualities of the products. © 2014 Taylor & Francis
Agency: GTR | Branch: BBSRC | Program: | Phase: Research Grant | Award Amount: 24.49K | Year: 2013
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Kapgate S.S.,Tamil Nadu Veterinary and Animal Sciences University |
Barbuddhe S.B.,Campus Management |
Kumanan K.,Tamil Nadu Veterinary and Animal Sciences University
Acta Virologica | Year: 2015
Increased globalisation, climatic changes and wildlife-livestock interface led to emergence of novel viral pathogens or zoonoses that have become serious concern to avian, animal and human health. High biodiversity and bird migration facilitate spread of the pathogen and provide reservoirs for emerging infectious diseases. Current classical diagnostic methods designed to be virus-specific or aim to be limited to group of viral agents, hinder identifying of novel viruses or viral variants. Recently developed approaches of next-generation sequencing (NGS) provide culture-independent methods that are useful for understanding viral diversity and discovery of novel virus, thereby enabling a better diagnosis and disease control. This review discusses the different possible steps of aNGS study utilizing sequence-independent amplification, high-throughput sequencing and bioinformatics approaches to identify novel avian viruses and their diversity. NGS lead to the identification of awide range of new viruses such as picobirnavirus, picornavirus, orthoreovirus and avian gamma coronavirus associated with fulminating disease in guinea fowl and is also used in describing viral diversity among avian species. The review also briefly discusses areas of viral-host interaction and disease associated causalities with newly identified avian viruses.