Tamil Nadu Rice Research Institute
Tamil Nadu Rice Research Institute
Govindasamy K.,Tamil Nadu Rice Research Institute |
Ebenezer J.,Tamil Nadu Agricultural University |
Sevugapperumal N.,Tamil Nadu Agricultural University |
Devi A.,Tamil Nadu Rice Research Institute
Indian Journal of Nematology | Year: 2016
Biopesticidal formulations of naturally occurring beneficial microorganisms are the most promising methods for more rational and safe crop management practices. Bacillus subtilis strains are important groups of natural antagonists that produce a broad spectrum of bioactive peptides with great potential for biocontrol of phytopathogens. Surfactin and iturin are antibiotic lipopeptides which imparts biocidal activity by direct suppression of plant-parasitic nematodes. Root-knot nematode, Meloidogyne incognita is an economically important pathogen of noni, a herbal medicine gaining popularity in India. In this investigation, endophytic strains of B. subtilis were isolated from noni plants and tested for their nematicidal activity against root knot nematode, in vitro. The genomic DNA of the Bacillus strains was isolated and amplified by PCR to identify antibiotic genes surfactin and iturin. The strain Bs 5, with high surfactin and iturin activity was found to suppress egg hatching and caused juvenile mortality. Using the ‘omic’ approaches effort were taken to understand pathogenicity and defence-related genes and proteins expressed during the three way interaction of host, pathogen and biocontrol agent during disease development. Protein profiling was done using (2-DE) two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were analyzed by mass spectrometry. Unique protein spots were excised and analyzed by MALDI-TOF MS/MS, followed by cross-species protein identification. A total of 15 different proteins were found to be differentially expressed. Proteomic investigations revealed that certain functionally important defense related proteins viz., Putative late blight resistance protein homolog, Toll-interleukin resistance domain containing protein, Translation Initiation factor IF1, Disease resistance protein putative Kalata- B1 and β-1,3-glucanase were induced by B. subtilis which are involved in the induction of defense response of host against the pathogen, M. incognita. © 2016, Nematological Society of India. All rights reserved.
Santha S.,Soil and Water Management Research Institute |
Vaithilingam R.,Tamil Nadu Rice Research Institute |
Karthikeyan A.,Regional Research Station
Vegetos | Year: 2016
In rice, a line x tester analysis with twelve high yielding genotypes viz., ADT 39, ADT 42, ADT 43, ADT (R) 45, ADT (R) 46, ADT (R) 47, TNAU Rice ADT 49, CO 47, ASD 16, TKM 11, TKM 12 and TRY 2 and five testers viz., Pusa 1460, Imp. Samba Mahsuri, Ajaya, IRBB 60 and IRBB 21 revealed that the ratio of GCA : SCA variances computed for all the six yield and yield component traits showed the predominance of non- additive gene action. Based on gca effects, ADT 43 and ADT (R) 46 were adjudged as the best combiners for five out of six biometrical traits including grain yield per plant. The parents ADT 42, Pusa 1460 and IRBB 60 possessed favourable gca effects for four biometrical traits including grain yield per plant. This was followed by ADT (R) 45, CO 47, ASD 16 and Imp. Samba Mahsuri which were considered as the next best general combiners since they exhibited desirable gca effects for three characters including hundred grain weight. ADT (R) 46/Pusa 1460 for grain yield per plant of the present study revealed the highest sca effect represent dominance and epistatic components of variation. © 2016, SciTechnol, All Rights Reserved.
PubMed | Tamil Nadu Rice Research Institute, Bangladesh Agricultural Research Institute, Indian Agricultural Research Institute, University of Hohenheim and 9 more.
Type: Journal Article | Journal: Global change biology | Year: 2016
South Asian countries will have to double their food production by 2050 while using resources more efficiently and minimizing environmental problems. Transformative management approaches and technology solutions will be required in the major grain-producing areas that provide the basis for future food and nutrition security. This study was conducted in four locations representing major food production systems of densely populated regions of South Asia. Novel production-scale research platforms were established to assess and optimize three futuristic cropping systems and management scenarios (S2, S3, S4) in comparison with current management (S1). With best agronomic management practices (BMPs), including conservation agriculture (CA) and cropping system diversification, the productivity of rice- and wheat-based cropping systems of South Asia increased substantially, whereas the global warming potential intensity (GWPi) decreased. Positive economic returns and less use of water, labor, nitrogen, and fossil fuel energy per unit food produced were achieved. In comparison with S1, S4, in which BMPs, CA and crop diversification were implemented in the most integrated manner, achieved 54% higher grain energy yield with a 104% increase in economic returns, 35% lower total water input, and a 43% lower GWPi. Conservation agriculture practices were most suitable for intensifying as well as diversifying wheat-rice rotations, but less so for rice-rice systems. This finding also highlights the need for characterizing areas suitable for CA and subsequent technology targeting. A comprehensive baseline dataset generated in this study will allow the prediction of extending benefits to a larger scale.
Ladhalakshmi D.,Directorate of Rice Research |
Laha G.S.,Directorate of Rice Research |
Singh R.,CCSHAU Rice Research Station |
Karthikeyan A.,Tamil Nadu Rice Research Institute |
And 4 more authors.
Phytoparasitica | Year: 2012
The intensity of rice false smut disease in selected states of northwest and south India was studied. In northern Indian states as a whole, disease incidence (percentage of false smut-infected tillers) varied from 2% to 75%. In the state of Haryana, maximum infection was recorded on hybrids like PA 6444 and PA 6129 while in Punjab state, 10-20% disease incidence was recorded in popular inbred rice varieties like PR 114, PA 116 and PAU 201. In the southern state of Tamil Nadu, the disease incidence varied from 5% to 85%. A heavy incidence of the disease was noticed in variety BPT 5204 and due to this, the air above the infected field gave a black smoky appearance from a distance as a result of release of spore mass in the atmosphere. In severe cases the number of infected grains reached even more than 100 per panicle. The pathogen Ustilaginoidea virens was isolated in potato dextrose agar medium and was characterized by both pathogenicity test and molecular analysis. Under glasshouse conditions, when a conidial suspension of the pathogen was injected during boot leaf stage of the rice variety TN1, typical smut balls were observed. The identity of the pathogen was further confirmed through polymerase chain reaction (PCR) analysis using U. virens-specific internal transcribed spacer (ITS) primers. The primer pair US 1-5/US3-3 and US2-5/US4-3 amplified 380 bp and 232 bp product, respectively, which are typical for the U. virens fungus. © 2012 Springer Science + Business Media B.V.
Perumalsamy S.,Tamil Nadu Agricultural University |
Bharani M.,Tamil Nadu Agricultural University |
Sudha M.,Tamil Nadu Agricultural University |
Nagarajan P.,Tamil Nadu Agricultural University |
And 4 more authors.
Plant Breeding | Year: 2010
Evolving high yielding rice genotypes with durable resistance to bacterial leaf blight (BLB) is pertinent considering the extensive damage caused by the disease in most of the rice growing regions. Two high yielding BLB susceptible indica rice cultivars, 'ADT43' and 'ASD16' popular among farmers and consumers across South India have been introgressed with three BLB resistance genes xa5, xa13 and Xa21 from isoline IRBB60 using functional markers. The F 2 populations of 500 plants from ADT43 × IRBB60 and 806 plants from ASD16 × IRBB60 crosses were screened for the presence of all the three resistance genes. Thirty genotypes with three genes in homozygous and 55 genotypes with two genes in homozygous and one gene in heterozygous condition were identified. These pyramided genotypes with two or three resistance genes exhibited high levels of resistance against two predominant Xanthomonas oryzae isolates of South India. Among the 30 pyramided genotypes (xa5 + xa13 + Xa21), 12 were found to be significantly high yielding with desirable agronomic characteristics and the selection efficiency of the present markers was hundred percent. © 2009 Blackwell Verlag GmbH.
Bharani M.,Tamil Nadu Agricultural University |
Nagarajan P.,Tamil Nadu Agricultural University |
Rabindran R.,Tamil Nadu Agricultural University |
Saraswathi R.,Tamil Nadu Rice Research Institute |
And 2 more authors.
Archives of Phytopathology and Plant Protection | Year: 2010
Marker assisted selection was employed to pyramid three bacterial blight resistance genes Xa21, xa13 and xa5 into high yielding susceptible rice cultivars ADT43 and ADT47. With the assistance of PCR markers, homozygous and heterozygous genotypes were identified in F2 generation of two crosses (ADT43 6 IRBB60 and ADT47 6 IRBB60) and goodness of fit was tested. Eighty nine plants from F3 generation of ADT43 6 IRBB60 were also screened for resistance genes.The genotypes carrying resistance genes in different combinations were identified. The pyramided lines showed a wider spectrum and higher level of resistance against two Xoo isolates under field conditions. © 2010 Taylor & Francis.
Sable A.,Tamil Nadu Agricultural University |
Suresh S.,Tamil Nadu Rice Research Institute |
Mohan Kumar S.,Tamil Nadu Agricultural University
Ecology, Environment and Conservation | Year: 2015
Antibiosis of five selected genotypes against brown planthopper (BPH) Nilaparvata lugens was studied under glass house conditions. Number of probing marks by BPH was higher and feeding rate and adult longevity was lower on all test genotypes as compared to susceptible check TNI. BPH produced highest number of probing marks on RP 2068-18-3-5 (27.88/plant). Feeding rate of BPH females was measured by honeydew excretion area over 24 hours. On test genotypes, feeding rate ranged from 58.13 to 277.50 mm2 and 22.63 to 178.88 mm2 on 40 and 60 day old plants respectively which were significantly lower than TNI. Longevity of adults was significantly lower ranging from 4.05 to 7.12 days on all genotypes compared to 9.69 days on TNI. Plant resistance appeared to be increasing with increasing age irrespective of resistance level. Copyright © EM International.
Pandian S.S.,Tamil Nadu Agricultural University |
Robin S.,Tamil Nadu Agricultural University |
Vinod K.K.,Tamil Nadu Agricultural University |
Vinod K.K.,Indian Agricultural Research Institute |
And 5 more authors.
Australian Journal of Crop Science | Year: 2011
Rice as the major staple food of the world although is high in calories but deficient in essential micronutrients. This deficiency can be tackled by biofortification of rice grains with enhanced micronutrient content. Field experiments were carried out across three locations involving 17 genotypes, with the objective of assessing the genotype × environment interaction for native soil micronutrient assimilation in rice kernels and the influence of intrinsic soil variables on micronutrient content in rice grains. Contents of Fe, Zn, Cu and Mn in the milled kernels were found to vary significantly among genotypes and locations. There was significant genotype-byenvironment interaction (GEI) for all the nutrients. Further, it was found that soil pH and soil available P had significant influence towards grain Fe content. Soil Zn availability and the electrical conductivity of the soil solution were found closely associated with grain Zn content. The two factors that regulated Cu and Mn content of rice grains were soil organic carbon content and soil Cu availability. The factorial regression (FR) approach to assess the role of significant intrinsic soil factor (s) on enrichment of specific micronutrient in the milled rice kernels showed differential genotype responses that ultimately determined grain micronutrient accumulation in each genotype. These determinants enabled prediction of genotypes suited to different environments. In certain cases, further examination of environmental co-factors is advocated to bring out a comprehensive picture of the micronutrient assimilation in milled rice grains.
Ramesh T.,Tamil Nadu Rice Research Institute |
Rathika S.,Tamil Nadu Rice Research Institute |
Parthipan T.,Tamil Nadu Rice Research Institute |
Ravi V.,Tamil Nadu Rice Research Institute
Legume Research | Year: 2016
Field experiment was conducted during rice fallow season (January-March), 2011 and 2012 to study the effect of DAP application to preceding rice in last irrigation and foliar nutrition to black gram on the productivity and profitability of black gram(ADT 3) under rice fallow condition. The results indicated that application of DAP at 50 kg/ha to preceding rice in last irrigation and foliar spray of DAP (2 per cent) and KCl (1 per cent) at 30 and 45 days after sowing to black gram registered higher plant height (24.6 cm) at 20 DAS number of pods per plant (18.4), grain yield (639 kg/ha), net returns (Rs.22290/ha) and additional income (Rs. 7563/ha). Application of DAP at 50 kg/ha in last irrigation to preceding rice produced significantly higher grain yield of succeeding black gram (613 kg/ha.) over control (488 kg/ha) which was comparable with foliar application of 2 per cent DAP and 1 per cent KCl to black gram. With reference to return per rupee invested, application of DAP at 50 kg/ha to preceding rice in last irrigation registered higher benefit cost ratio of 3.56 in black gram. © 2016, Agricultural Research Communication Centre. All rights reserved.
Maavimani M.,Agricultural College and Research Institute |
Saraswathi R.,Tamil Nadu Rice Research Institute
Indian Journal of Genetics and Plant Breeding | Year: 2014
The effect of high temperature at anthesis on spikelet fertility was studied in 22 rice genotypes selected from 98 heat tolerant genotypes. Genotypes were grown in field under natural conditions in Randomized Block Design replicated twice till vegetative stage. Before booting stage, few plants were transferred to pots and placed in polyhouse for high temperature treatments. On the first day of anthesis (i.e. the appearance of anthers), plants were transferred into polyhouse with temperatures gradually increasing from 29°C to 40°C and maintained at 40°C until 15 h, with a relative humidity of 75 percent. Seven genotypes viz., IR 86970-145-1, IR86977-140-2, IR 86977-141-3, IR86977-95-1, IR 86970-126-3, IR 86977-92-3 and IR 86977-104-1 exhibited 20 to 40 per cent spikelet fertility after exposure to high temperature of 40°C. The pollen fertility ranged from 39.0 to 90.5 per cent. The genotype IR 86970-145-1 had significant value for anther length (2.125) and anther breadth (0.320). Anther dimensions seem to have an influence on anther dehiscence, pollen and spikelet fertility. All other genotypes were found to be sterile with no seed set. Exposure to high temperature during heading has significantly reduced the spikelet fertility but there were genotypic differences. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.