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Swarna J.,Tal Samy Center For Plant Tissue Culture And Molecular Biology | Lokeswari T.S.,Sri Ramachandra University | Smita M.,CSIR - National Chemical Laboratory | Ravindhran R.,Tal Samy Center For Plant Tissue Culture And Molecular Biology
Food Chemistry | Year: 2013

Talinum triangulare (Jacq.) Willd is an erect, succulent, perennial herb belonging to the family Portulacaceae. Under extreme environmental conditions, the plant produces betalain pigments which get accumulated in the stem region. Hence, in the present study, the betaxanthin and betacyanin patterns from different samples of T. triangulare have been investigated by applying high-performance liquid chromatography photo-diode array detection (HPLC-PDA) coupled with positive ion electro-spray mass spectrometry. Two betacyanins and two betaxanthins were identified in aqueous methanolic extract of flower, stem and leaf. Betanin, isobetanin, immonium conjugates of betalamic acid with dopamine and tyrosine were elucidated. The total betalain content was estimated by photometric analysis. In vitro antioxidant activity for the betalain extract determined by various methods revealed potent scavenging ability. The current work may possibly be considered beneficial in utilisation of the plant T. triangulare as a natural colourant in food and beverage industries. © 2013 Elsevier Ltd. Source


Swarna J.,Tal Samy Center For Plant Tissue Culture And Molecular Biology | Ravindhran R.,Tal Samy Center For Plant Tissue Culture And Molecular Biology | Lokeswari T.S.,Sri Ramachandra University
South African Journal of Botany | Year: 2015

Talinum triangulare is a medicinal herb known to have originated from tropical Africa and is now widely cultivated in the humid tropical countries. The characterization of plant germplasm using molecular descriptors aids in describing the genotypic traits of the plant T. triangulare. Ten different accessions of T. triangulare were collected from nine districts of Tamil Nadu. High quality genomic DNA was isolated from the different samples, checked for purity and quantified. The DNA samples were subjected to PCR amplification using RAPD markers and by DNA barcoding technique. RAPD fingerprints were generated for the 10 different accessions of T. triangulare for the first time. Ten random decamer primers resulted in 73.08% of polymorphism by producing a total of 78 fragments, of which, 57 bands were determined as polymorphic loci. Phylogenetic relationship and intra-specific variation among the samples were established by constructing a dendrogram based on Jaccard's coefficient. DNA barcoding studies included the amplification of chloroplast large subunit of ribulose-bisphosphate carboxylase (rbcL), trnH-psbA intergenic spacer (trnH. -psbA), maturase K (matK) and nuclear internal transcribed spacer (ITS). The amplicons were gel eluted, sequenced and checked for homology by using the BLAST tool. However, no variation was detected among the samples after sequencing, proving that the accessions were identical at the genetic level. © 2014 South African Association of Botanists. Source


Swarna J.,Tal Samy Center For Plant Tissue Culture And Molecular Biology | Ravindhran R.,Tal Samy Center For Plant Tissue Culture And Molecular Biology
Plant Growth Regulation | Year: 2013

Talinumtriangulare is an important medicinal herb used traditionally in the treatment of various diseases. The present study was intended to develop a rapid and efficient protocol for indirect organogenesis from leaf discs and transverse thin cell layer (tTCL) of internodal explants of T. triangulare. Best callusing response (100 %) was observed with tTCL explants on Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzyl amino purine and 5. 37 μM α-naphthalene acetic acid (NAA). High frequency shoot regeneration (96. 67 %) was obtained from tTCL derived calli on MS medium supplemented with a combination of 0.45 μM thidiazuron and 0. 27 μM NAA, by producing 9. 20 ± 0.35 shoots with a shoot length of 2.74 ± 0. 03 cm. In vitro rooting of the microshoots was recorded on half-strength MS medium containing 2. 46 μM indole-3-butyric acid by eliciting 15.20 ± 0.27 roots with a length of 4. 25 ± 0. 11 cm. The rooted shoots were acclimatized on garden soil, sand and coco pith (1:1:3 v/v) planting substrate. The plantlets were successfully established under field conditions with 100 % survival rate. The hardened plants exhibited homogeneity and no observable morphological variations were detected among the regenerants and the mother plants of T. triangulare. © 2013 Springer Science+Business Media Dordrecht. Source

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