Takii and Co.

Konan, Japan

Takii and Co.

Konan, Japan
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Noda T.,Kumamoto Prefectural Agriculture Research Center | Iimure K.,Kumamoto Prefectural Agriculture Research Center | Okamoto S.,Takii and Company | Saito A.,Kumamoto Prefectural Agriculture Research Center
Bioscience, Biotechnology and Biochemistry | Year: 2017

Browning of plant tissue is generally considered attributable to enzymatic oxidation by polyphenol oxidase (PPO). Electrophoresis followed by activity staining has been used as an effective procedure to visually detect and isolate isozymes; however, it has not been applied for examination of various PPO isozymes in lettuce. Our study demonstrated that different lettuce PPO isozymes could be detected at different pH in active staining, and multiple isozymes were detected only under alkaline conditions. As a result, we concluded that activity staining with approximately pH 8 enabled to detect various PPO isozymes in lettuce. By expression analysis of the PPO isozymes after wounding, PPO isozymes that correlated with time-course of tissue browning were detected. The wound-induced PPO may play a key role in enzymatic browning. © 2017 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

Kishimoto K.,Japan National Agriculture and Food Research Organization | Maeda H.,Takii and Company Ltd | Haketa T.,Takii and Company Ltd | Oyama-Okubo N.,Japan National Agriculture and Food Research Organization
Journal of the Japanese Society for Horticultural Science | Year: 2014

Ornamental cabbage (Brassica oleracea var. acephala f. tricolor) cultivars of Japanese seed companies are grown in Europe. Unpleasant odors from cut flowers have become a problem in this region. We investigated volatiles emitted from cut flowers of 'Hatsubeni' and 'Haresugata', two major Japanese ornamental cabbage cultivars in Europe, by gas chromatography-mass spectrometry (GC-MS). Dimethyl disulfide was identified as the major odor-active component emerging from plants as well as from vase water. Vase water was a major source of unpleasant scents, and odor development was prevented by changing the water frequently. We tested the effects of potential suppressors of dimethyl disulfide emission from cut flowers applied to vase water. Cyprodinil, an inhibitor of the synthesis of dimethyl disulfide from methionine, and aminooxyacetic acid, an inhibitor of enzymatic reactions involved in dimethyl disulfide biosynthesis, did not show any effects. In contrast, isothiazolinonic germicide, a cut-flower preservative, inhibited the rot of cut ends of flower stems and reduced dimethyl disulfide emission from cut flowers by 30-40%. In addition, this germicide significantly inhibited emissions from vase water. Isothiazolinonic germicide is a promising candidate inhibitor of unpleasant scents from cut flowers of ornamental cabbage. © 2014 The Japanese Society for Horticultural Science (JSHS), All right reserved.

Oikawa E.,Tohoku University | Takuno S.,Tohoku University | Takuno S.,University of California at Irvine | Izumita A.,Sakata Seed Corporation | And 4 more authors.
Molecular Breeding | Year: 2011

In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i. e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds. © 2010 Springer Science+Business Media B.V.

Shichij C.,Kobe University | Takahashi-Asami M.,Kobe University | Takahashi-Asami M.,Takii and Co. | Nagatoshi Y.,Kobe University | And 2 more authors.
Plant Signaling and Behavior | Year: 2010

Progression of the apical hook of tomato, Solanum lycopersicum, exaggerated by phytochrome mediation at the early germination stage, is followed in detail macroscopically and anatomically, and its proposed significance, i.e., survival by securing the seed coat release in the field, is reinforced by new findings. Furthermore, after self-release or artificial removal of the seed coat and the endosperm, no hook exaggeration occurs any more. Similar light-induced hook exaggeration (LIHE) is also found in carrot, parsley and Cryptotaenia japonica, which share some seed characteristics with tomato. These findings also support the abovestated significance. © 2010 Landes Bioscience.

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