Takanashi Milk Products Co.

Yokohama-shi, Japan

Takanashi Milk Products Co.

Yokohama-shi, Japan
SEARCH FILTERS
Time filter
Source Type

PubMed | University of Turku and Takanashi Milk Products Co.
Type: | Journal: European journal of nutrition | Year: 2016

Japanese cedar pollinosis (JCP) is a challenging public health problem in Japan. Altered gut microbiota is associated with several diseases, including allergic diseases. However, only a few studies have focused on JCP and the underlying mechanisms for probiotic effects remain unclear. In addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in JCP.Faecal samples from JCP subjects were collected before and after treatment with (n=14) and without (n=11) LGG-TMC0356-fermented milk for 10weeks. Gut microbiota composition was characterized from faecal DNA using sequencing of 16S rRNA genes.16S rRNA-based operational taxonomic unit clustering of the microbiota revealed that LGG-TMC0356-fermented milk significantly altered gut microbiota after 10weeks of milk consumption, and eight dominant genera of microbes were detected. During the JCP season, the Bacteroidetes/Firmicutes ratio, when compared to baseline, was significantly decreased in subjects at end of the study. Bacteroidetes showed positive correlation with LDL- and HDL-cholesterol levels, whereas Firmicutes showed negative correlation with total cholesterol, LDL- and HDL- cholesterol.The altered gut microbiota through supplementation of fermented milk containing the study probiotics may be a prospective target for protection against JCP, with beneficial effects on blood lipid levels.


Yoda K.,Takanashi Milk Products Company Ltd | Sun X.,University of Tennessee at Knoxville | Kawase M.,Takanashi Milk Products Company Ltd | Kubota A.,Takanashi Milk Products Company Ltd | And 6 more authors.
British Journal of Nutrition | Year: 2015

Lactobacillus rhamnosus GG, Lactobacillus paracasei TMC0409, Streptococcus thermophilus TMC1543 and whey proteins were used to prepare fermented milk. For the experiment aP2-agouti transgenic mice were pre-treated with a high-sucrose/high-fat diet for 6 weeks to induce obesity. The obese mice were fed a diet containing 1·2 % Ca and either non-fat dried milk (NFDM) or probiotic-fermented milk (PFM) with nutritional energy restriction for 6 weeks. The animals were examined after the treatment for changes in body weight, fat pad weight, fatty acid synthase (FAS) activity, lypolysis, the expression levels of genes related to lipid metabolism, insulin sensitivity in adipocytes and skeletal muscle and the presence of biomarkers for oxidative and inflammatory stress in plasma. It was found that the PFM diet significantly reduced body weight, fat accumulation, and adipocyte FAS activity, and increased adipocyte lipolysis as compared with the effects of the NFDM diet (P< 0·05). The adipose tissue gene expression of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) was significantly suppressed in mice that were fed PFM as compared with those that were fed NFDM (P< 0·05). PFM caused a greater up-regulation of skeletal muscle PPARα, PPARδ, uncoupling protein 3 (UCP3) and GLUT4 expression and a significant decrease in the plasma concentration of insulin, malondialdehyde, TNF-α, monocyte chemotactic protein-1 and C-reactive protein as compared with the effects of NFDM (P< 0·05). Fermentation of milk with selected probiotics and supplementation of milk with whey proteins may thus enhance anti-obesity effects of Ca and dairy products by the suppression of adipose tissue lipogenesis, activation of fat oxidation in skeletal muscle and reduction of oxidative and inflammatory stress. © 2015 The Authors.


Honda K.,Wayo Women's University | Moto M.,Wayo Women's University | Uchida N.,Wayo Women's University | He F.,Takanashi Milk Products Co. | Hashizume N.,Wayo Women's University
Journal of Clinical Biochemistry and Nutrition | Year: 2012

The antidiabetic effects of lactic acid bacteria were investigated using mice. In Experiment 1, normal ICR mice were loaded with sucrose or starch with or without viable Lactobacillus rhamnosus GG cells. GG significantly inhibited postprandial blood glucose levels when administered with sucrose or starch. In Experiment 2, KK-Ay mice, a model of genetic type 2 diabetes, were given a basal diet containing viable GG cells or viable Lactobacillus delbrueckii subsp. bulgaricus cells for 6 weeks. Viable GG cells significantly inhibited fasting blood glucose, postprandial blood glucose in a glucose tolerance test and HbA1c. Such effects were not shown by viable L. bulgaricus cells. In Experiment 3, the KK-Ay mice were given a basal diet containing viable GG cells or heat-treated GG cells for 3 weeks. The viable GG cells significantly suppressed fasting blood glucose and impaired glucose tolerance, but the heat-treated GG showed no effects. These results demonstrated that GG decreased the postprandial blood glucose in ICR mice, and that the antidiabetic activity of lactic acid bacteria on the KK-Ay mice differed depending on the bacterial strain and whether the bacterium is viable when it arrives in the intestine. In the present study, we conclude that the antidiabetic activity may result from continuous inhibition of the postprandial blood glucose through suppression of glucose absorption from the intestine. These findings indicate that specific strains of lactic acid bacterium can be expected to be beneficial for the management of type 2 diabetes. ©2012 JCBN.


Yoda K.,Takanashi Milk Products Co. | Miyazawa K.,Takanashi Milk Products Co. | Hosoda M.,Takanashi Milk Products Co. | Hiramatsu M.,Takanashi Milk Products Co. | And 2 more authors.
European Journal of Nutrition | Year: 2014

Background: Fermented milk is considered one of the best sources for efficient consumption of probiotic strains by hosts to promote good health. The purpose of this study was to investigate the effects of orally administering LGG-fermented milk (LGG milk) on intestinal inflammation and injury and to study the mechanisms of LGG milk's action. Methods: LGG milk and non-LGG-fermented milk (non-LGG milk) were administered through gavage to mice before and during dextran sodium sulfate (DSS)-induced intestinal injury and colitis. Inflammatory/injury score and colon length were assessed. Intestinal epithelial cells were treated with the soluble fraction of LGG milk to detect its effects on the epidermal growth factor receptor (EGFR) and its downstream target, Akt activation, cytokine-induced apoptosis, and hydrogen peroxide (H 2O2)-induced disruption of tight junctions. Results: LGG milk treatment significantly reduced DSS-induced colonic inflammation and injury, and colon shortening in mice, compared to that in non-LGG milk-treated and -untreated mice. The soluble fraction of LGG milk, but not non-LGG milk, stimulated the activation of EGFR and Akt in a concentration-dependent manner, suppressed cytokine-induced apoptosis, and attenuated H2O 2-induced disruption of tight junction complex in the intestinal epithelial cells. These effects of LGG milk were blocked by the EGFR kinase inhibitor. LGG milk, but not non-LGG milk, contained two soluble proteins, p40 and p75, that have been reported to promote survival and growth of intestinal epithelial cells through the activation of EGFR. Depletion of p40 and p75 from LGG milk abolished the effects of LGG milk on prevention of cytokine-induced apoptosis and H2O2-induced disruption of tight junctions. Conclusions: These results suggest that LGG milk may regulate intestinal epithelial homeostasis and potentially prevent intestinal inflammatory diseases through activation of EGFR by LGG-derived proteins. © 2013 Springer-Verlag Berlin Heidelberg.


Kawase M.,Takanashi Milk Products Co. | He F.,Takanashi Milk Products Co. | Miyazawa K.,Takanashi Milk Products Co. | Kubota A.,Takanashi Milk Products Co. | And 2 more authors.
FEMS Microbiology Letters | Year: 2012

The present study was conducted to test the ability of probiotic lactobacilli to alter age-related immunosenescence in host animals. Senescence-accelerated mouse prone 1 mice were orally fed heat-killed Lactobacillus gasseri TMC0356 (TMC0356) for 4 and 8 weeks at dosages of 10 mg day -1 after a 16-week period of prefeeding with a standard diet. After 4 and 8 weeks of TMC0356 intervention, splenic activation of natural killer (NK) cells and mRNA expression of cytokines and other immune molecules in the lungs were analysed. After 4 and 8 weeks, splenic NK cell activities were significantly higher in the TMC0356-fed mice compared with control mice (P < 0.05). After 4 weeks, mRNA expression of interleukin-2 and interferon-(α and β) receptor 1 in lung cells isolated from the TMC0356-fed mice also increased significantly compared with that in lung cells from the control mice (P < 0.05). These results suggest that lactobacilli, especially certain selected strains, might enhance cell-mediated immunity in host animals and thereby alter age-related immunosenescence. © 2012 Federation of European Microbiological Societies.


Kawase M.,Takanashi Milk Products Co. | He F.,Takanashi Milk Products Co. | Kubota A.,Takanashi Milk Products Co. | Yoda K.,Takanashi Milk Products Co. | And 2 more authors.
FEMS Immunology and Medical Microbiology | Year: 2012

This study investigated whether heat-killed Lactobacillus protects host animal against influenza virus infection and stimulates their immunity. Heat-killed Lactobacillus gasseri TMC0356 was orally administered to BALB/c mice for 19 days; the mice were intranasally infected with Flu A/PR/8/34 (H1N1) on day 14, and clinical symptoms were monitored. After 6 days, the mice were sacrificed, and pulmonary virus titres were determined. Splenic activation of natural killer (NK) cells and the mRNA expression of cytokines and other immune molecules in the lung and Peyer's patch (PP) were analysed. Clinical symptom scores of mice orally fed TMC0356 ameliorated significantly (P < 0.01); their pulmonary virus titres decreased significantly compared with those of control mice (P < 0.05); their mRNA expression of interleukin (IL)-12, IL-15 and IL-21 in PP and the pulmonary mRNA expression of IFN-γ, TNF, IL-12a, IL-12rbl, IL-2rb and perforin 1 increased significantly (P < 0.05). Oral administration of heat-killed lactobacilli may protect against influenza virus infection by stimulating local and systemic immune responses. Cellular components of lactobacilli may be pivotal in protecting against viral infection by enhancing gut and respiratory immune responses. © 2012 Federation of European Microbiological Societies.


Kawase M.,Takanashi Milk Products Co. | He F.,Takanashi Milk Products Co. | Kubota A.,Takanashi Milk Products Co. | Harata G.,Takanashi Milk Products Co. | Hiramatsu M.,Takanashi Milk Products Co.
Letters in Applied Microbiology | Year: 2010

Aims: Our study was conducted to evaluate the potent protective effects of oral administration of probiotic Lactobacillus strains against influenza virus (Flu) infection in a mouse model. Method and Results: Lyophilized Lactobacillus rhamnosus GG (LGG) and Lactobacillus gasseri TMC0356 (TMC0356) were orally administered to BALB/c mice for 19 days. The test mice were intranasally infected with Flu A/PR/8/34 (H1N1) on day 14, and any changes in clinical symptoms were monitored. After 6 days of infection, the mice were killed and pulmonary virus titres were determined. The clinical symptom scores of mice administered oral LGG and TMC0356 were significantly ameliorated, compared to those of the control mice (P < 0·01). The pulmonary virus titres of the mice fed LGG and TMC0356 were also significantly decreased compared to those of control mice (P < 0·05). Conclusions: These results indicate that oral administration of lactobacilli, such as LGG and TMC0356, might protect a host animal against Flu infection. Significance and Impact of the Study: These results demonstrate that oral administration of selected lactobacilli might protect host animals from Flu infection by interactions with gut immunity. © 2010 The Society for Applied Microbiology.


Miyazawa K.,Takanashi Milk Products Co. | Kawase M.,Takanashi Milk Products Co. | Kubota A.,Takanashi Milk Products Co. | Yoda K.,Takanashi Milk Products Co. | And 3 more authors.
Beneficial Microbes | Year: 2015

This double-blind, placebo-controlled clinical trial was conducted to test whether Lactobacillus gasseri TMC0356 (TMC0356) can modify the immune response in the elderly. Heat-killed TMC0356 or placebo was orally administered to 28 healthy subjects aged 50-70 years old for 4 weeks at a dosage of 1.0×109 cfu/day. Peripheral blood mononuclear cells (PBMCs) were collected from the subjects before and after the study completion, together with general health and blood examination records. Isolated PBMCs were examined for the number of T cells, CD8+CD28+ cells, native T cells, B cells, natural killer (NK) cells and the ratios of CD4/CD8 T cells and native/memory T cells. NK cell activation and concanavalin A-induced lymphocyte transformation of the isolated PBMCs were also examined. The number of CD8+ T cells significantly increased in the subjects after TMC0356 oral administration (P < 0.05). Furthermore, the population of CD8+CD28+ T cells and the amount of lymphocyte transformation both significantly decreased in PBMCs from the placebo group (P < 0.05). However, such changes were not observed in the subjects exposed to TMC0356. These results suggest that TMC0356 can increase the number of CD8+ T cells and reduce CD28 expression loss in CD8+ T cells of the elderly. The effect of TMC0356 on immune responses in the elderly may enhance their natural defence mechanisms against pathogenic infections. © 2015 Wageningen Academic Publishers.


Yoda K.,Takanashi Milk Products Company Ltd | He F.,Takanashi Milk Products Company Ltd | Kawase M.,Takanashi Milk Products Company Ltd | Miyazawa K.,Takanashi Milk Products Company Ltd | Hiramatsu M.,Takanashi Milk Products Company Ltd
Journal of Microbiology, Immunology and Infection | Year: 2014

Background: Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in human. This study was conducted to investigate the ability of orally administrated probiotic lactobacilli to protect hosts from EPEC infection via enhancement of immune responses. Methods: Lyophilized Lactobacillus gasseri TMC0356 (TMC0356) was orally administered to Institute of Cancer Research (ICR) mice and Sprague Dawley (SD) rats for 11 and 7 days, respectively. These tested mice and rats were intraperitoneally injected with EPEC. Body weight, general symptoms (piloerection, soft stool, diarrhea, and anal hyperemia), and mortality of the tested mice were observed. Peritoneal macrophages were extracted from peritoneal cavity of tested rats, and their phagocytosis and cytokine production were analyzed. Results: Oral administration of TMC0356 accelerated the disappearance of general symptoms and reduced mortality of EPEC-infected mice in the early phase. Peritoneal macrophages from rats orally administered with TMC0356 showed significant increases in phagocytic activity (p<0.05) and interleukin (IL)-6 production (p<0.01) compared to those from control rats. Tumor necrosis factor-α and production of IL-1β, IL-10, and IL-12 slightly increased, although the changes were not statistically significant. Conclusion: These results suggest that some of selected probiotic lactobacilli may, at least partly, protect hosts from EPEC infection by the enhancement of innate immunity of host and attenuate symptoms caused by the infection. © 2012.


Patent
Takanashi Milk Products Co. | Date: 2012-07-11

An object is to provide a pasteurized fresh cheese which has a smooth texture and a good taste, shows little water release, scarcely shows a lowering in pH or acidity and has a long best-before period. The objective is achieved by a method for processing a pasteurized fresh cheese comprising the steps of:(a) coagulating a starting milk material to obtain a curd;(b) adding a stabilizer to the curd;(c) pasteurizing the curd by heating; and,(d) separating a whey from the curd to obtain a cheese curd.

Loading Takanashi Milk Products Co. collaborators
Loading Takanashi Milk Products Co. collaborators