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Yokohama-shi, Japan

Suginaka T.,Takanashi Milk Products Co.
Animal Science Journal | Year: 2012

We collected 17 milk samples at 1day to 6months post partum from one captive okapi (Okapia johnstoni). Milks were examined for nutrient composition, including water, fat, crude protein, carbohydrates, ash and five minerals. The average values of the concentrations were 79.2±3.0% SD water, 8.5±3.4% fat, 7.5±1.1% crude protein, 3.6±0.6% carbohydrate, 1.2±0.1% ash, 263.7±47.3mg/100g calcium, 183.0±40.0mg/100g phosphorus, 95.2±17.6mg/100g sodium, 74.4±34.3mg/100g potassium and 28.4±4.4mg/100g magnesium, respectively. The protein concentration increased at late lactation (P<0.01). The contents of sodium and potassium were rather high in the early lactation period, while those of calcium and phosphorus were rather low in the early lactation period. During the course of lactation in the first week post partum and the protein concentration was high at 1day post partum. We compare our findings with the American Association of Zoos & Aquariums's Okapi Species Survival Plan Hand-rearing Protocol, and recommend that nutrient composition of the formula for okapi calves is developed using the present study as a guide. © 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science.

Honda K.,Wayo Womens University | Moto M.,Wayo Womens University | Uchida N.,Wayo Womens University | He F.,Takanashi Milk Products Co. | Hashizume N.,Wayo Womens University
Journal of Clinical Biochemistry and Nutrition | Year: 2012

The antidiabetic effects of lactic acid bacteria were investigated using mice. In Experiment 1, normal ICR mice were loaded with sucrose or starch with or without viable Lactobacillus rhamnosus GG cells. GG significantly inhibited postprandial blood glucose levels when administered with sucrose or starch. In Experiment 2, KK-Ay mice, a model of genetic type 2 diabetes, were given a basal diet containing viable GG cells or viable Lactobacillus delbrueckii subsp. bulgaricus cells for 6 weeks. Viable GG cells significantly inhibited fasting blood glucose, postprandial blood glucose in a glucose tolerance test and HbA1c. Such effects were not shown by viable L. bulgaricus cells. In Experiment 3, the KK-Ay mice were given a basal diet containing viable GG cells or heat-treated GG cells for 3 weeks. The viable GG cells significantly suppressed fasting blood glucose and impaired glucose tolerance, but the heat-treated GG showed no effects. These results demonstrated that GG decreased the postprandial blood glucose in ICR mice, and that the antidiabetic activity of lactic acid bacteria on the KK-Ay mice differed depending on the bacterial strain and whether the bacterium is viable when it arrives in the intestine. In the present study, we conclude that the antidiabetic activity may result from continuous inhibition of the postprandial blood glucose through suppression of glucose absorption from the intestine. These findings indicate that specific strains of lactic acid bacterium can be expected to be beneficial for the management of type 2 diabetes. ©2012 JCBN.

Yoda K.,Takanashi Milk Products Co. | Miyazawa K.,Takanashi Milk Products Co. | Hosoda M.,Takanashi Milk Products Co. | Hiramatsu M.,Takanashi Milk Products Co. | And 2 more authors.
European Journal of Nutrition | Year: 2014

Background: Fermented milk is considered one of the best sources for efficient consumption of probiotic strains by hosts to promote good health. The purpose of this study was to investigate the effects of orally administering LGG-fermented milk (LGG milk) on intestinal inflammation and injury and to study the mechanisms of LGG milk's action. Methods: LGG milk and non-LGG-fermented milk (non-LGG milk) were administered through gavage to mice before and during dextran sodium sulfate (DSS)-induced intestinal injury and colitis. Inflammatory/injury score and colon length were assessed. Intestinal epithelial cells were treated with the soluble fraction of LGG milk to detect its effects on the epidermal growth factor receptor (EGFR) and its downstream target, Akt activation, cytokine-induced apoptosis, and hydrogen peroxide (H 2O2)-induced disruption of tight junctions. Results: LGG milk treatment significantly reduced DSS-induced colonic inflammation and injury, and colon shortening in mice, compared to that in non-LGG milk-treated and -untreated mice. The soluble fraction of LGG milk, but not non-LGG milk, stimulated the activation of EGFR and Akt in a concentration-dependent manner, suppressed cytokine-induced apoptosis, and attenuated H2O 2-induced disruption of tight junction complex in the intestinal epithelial cells. These effects of LGG milk were blocked by the EGFR kinase inhibitor. LGG milk, but not non-LGG milk, contained two soluble proteins, p40 and p75, that have been reported to promote survival and growth of intestinal epithelial cells through the activation of EGFR. Depletion of p40 and p75 from LGG milk abolished the effects of LGG milk on prevention of cytokine-induced apoptosis and H2O2-induced disruption of tight junctions. Conclusions: These results suggest that LGG milk may regulate intestinal epithelial homeostasis and potentially prevent intestinal inflammatory diseases through activation of EGFR by LGG-derived proteins. © 2013 Springer-Verlag Berlin Heidelberg.

Harata G.,Takanashi Milk Products Co. | He F.,Takanashi Milk Products Co. | Hiruta N.,Shinshu University | Kawase M.,Takanashi Milk Products Co. | And 3 more authors.
World Journal of Microbiology and Biotechnology | Year: 2011

We conducted a study to evaluate the possibility that intranasal administration of a new probiotic strain Lactobacillusgasseri TMC0356 (TMC0356) may protect host animals from influenza virus (IFV) infection, which was indicated by enhanced respiratory immune responses in a mouse model. After 3 days of exposure to TMC0356, BALB/c mice were intranasally infected with IFVA/PR/8/34 (H1N1). Lung cells were isolated from the tested mice and evaluated for cytotoxicity against YAC-1 cells. After intranasal treatment with TMC0356, mice showed a lower morbidity and higher survival rate compared to control mice (P < 0.05). The cytotoxicity of lung cells isolated from mice after intranasal treatment against YAC-1 cells was statistically higher than that of lung cells isolated from control mice (P < 0.05). Intranasal administration of TMC0356 significantly increased mRNA expression of interleukin (IL)-1β, tumor necrosis factor, IL-10, and monocyte chemotactic protein-1 (P < 0.01). These results suggest that intranasal administration of TMC0356 may protect the host animal from IFV infection. They also indicate that TMC0356 can enhance respiratory cell-mediated immune responses of host animals characteristically with up-regulated activation of lung natural killer cells. Further studies will evaluate the possible role of the immune stimulatory effects of TMC0356 within the protective effects of this bacterium against IFV, as observed in the present study. © 2010 Springer Science+Business Media B.V.

Yoda K.,Takanashi Milk Products Co. | He F.,Takanashi Milk Products Co. | Kawase M.,Takanashi Milk Products Co. | Miyazawa K.,Takanashi Milk Products Co. | Hiramatsu M.,Takanashi Milk Products Co.
Journal of Microbiology, Immunology and Infection | Year: 2014

Background: Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in human. This study was conducted to investigate the ability of orally administrated probiotic lactobacilli to protect hosts from EPEC infection via enhancement of immune responses. Methods: Lyophilized Lactobacillus gasseri TMC0356 (TMC0356) was orally administered to Institute of Cancer Research (ICR) mice and Sprague Dawley (SD) rats for 11 and 7 days, respectively. These tested mice and rats were intraperitoneally injected with EPEC. Body weight, general symptoms (piloerection, soft stool, diarrhea, and anal hyperemia), and mortality of the tested mice were observed. Peritoneal macrophages were extracted from peritoneal cavity of tested rats, and their phagocytosis and cytokine production were analyzed. Results: Oral administration of TMC0356 accelerated the disappearance of general symptoms and reduced mortality of EPEC-infected mice in the early phase. Peritoneal macrophages from rats orally administered with TMC0356 showed significant increases in phagocytic activity (p<0.05) and interleukin (IL)-6 production (p<0.01) compared to those from control rats. Tumor necrosis factor-α and production of IL-1β, IL-10, and IL-12 slightly increased, although the changes were not statistically significant. Conclusion: These results suggest that some of selected probiotic lactobacilli may, at least partly, protect hosts from EPEC infection by the enhancement of innate immunity of host and attenuate symptoms caused by the infection. © 2012.

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