Tzeng T.-F.,Pao Chien Hospital Pingtung County |
Chang C.J.,China Medical University at Taichung |
Liu I.-M.,Tajen University
Phytotherapy Research | Year: 2014
We investigated the effects of 6-gingerol ((S)-5-hydroxy-1-(4-hydroxy-3- methoxyphenyl)-3-decanone) on the inhibition of rosiglitazone (RGZ)-induced adipogenesis in 3T3-L1 cells. The morphological changes were photographed based on staining lipid accumulation by Oil-Red O in RGZ (1 μmol/l)-treated 3T3-L1 cells without or with various concentrations of 6-gingerol on differentiation day 8. Quantitation of triglycerides content was performed in cells on day 8 after differentiation induction. Differentiated cells were lysed to detect mRNA and protein levels of adipocyte-specific transcription factors by real-time reverse transcription-polymerase chain reaction and Western blot analysis, respectively. 6-gingerol (50 μmol/l) effectively suppressed oil droplet accumulation and reduced the sizes of the droplets in RGZ-induced adipocyte differentiation in 3T3-L1 cells. The triglyceride accumulation induced by RGZ in differentiated 3T3-L1 cells was also reduced by 6-gingerol (50 μmol/l). Treatment of differentiated 3T3-L1 cells with 6-gingerol (50 μmol/l) antagonized RGZ-induced gene expression of peroxisome proliferator-activated receptor (PPAR)γ and CCAAT/enhancer-binding protein α. Additionally, the increased levels of mRNA and protein in adipocyte-specific fatty acid binding protein 4 and fatty acid synthase induced by RGZ in 3T3-L1 cells were decreased upon treatment with 6-gingerol. Our data suggests that 6-gingerol may be beneficial in obesity, by reducing adipogenesis partly through the down-regulating PPARγ activity. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.
Pan H.-W.,Kaohsiung Veterans General Hospital |
Li S.-C.,Academia Sinica, Taiwan |
Tsai K.-W.,Kaohsiung Veterans General Hospital |
Tsai K.-W.,Tajen University
Current Pharmaceutical Design | Year: 2013
Gastric carcinogenesis is a complex multistep process involving genetic dysregulation of proto-oncogenes and tumorsuppressor genes, and has recently entered the era of microRNAs (miRNAs), a class of small non-coding RNAs that posttranscriptionally regulate gene expression and control various cellular functions. MicroRNAs are small (approximately 22 nucleotides) non-coding RNAs that play fundamental roles in diverse biological and pathological processes, including cell proliferation, differentiation, apoptosis, and carcinogenesis. MicroRNAs have been revealed to be involved in various stages of cancer development, showing that abnormal miRNA expressions play critical roles in modulating expression of known oncogenes or tumor suppressor genes during cancer progression. Therefore, microRNAs can perform the function of onco-miRs or tumor-suppressor-miRs in gastric carcinogenesis. This review summarizes a current understanding of the connection between miRNAs and gastric cancer. Additionally, this paper outlines the applications of miRNAs in clinical practice, such as diagnosis, prognosis, detection, and therapy of gastric cancer. © 2013 Bentham Science Publishers.
Chang J.S.,Kaohsiung Medical University |
Wang K.C.,Kaohsiung Medical University |
Yeh C.F.,Kaohsiung Medical University |
Shieh D.E.,Tajen University |
Chiang L.C.,Kaohsiung Medical University
Journal of Ethnopharmacology | Year: 2013
Ethnopharmacological relevance: Ginger, Zingiber officinale Roscoe, is a common spice and also a widely used medicinal plant in ancient China. Ginger is an ingredient of Ge-Gen-Tang (Kakkon-to; GGT). GGT has been proved to have antiviral activity against human respiratory syncytial virus (HRSV). However, it is unknown whether ginger is effective against HRSV. Aim of the study: To find a readily available agent to manage HRSV infection, the authors tested the hypothesis that ginger can effectively decrease HRSV-induced plaque formation in respiratory mucosal cell lines. Materials and methods: Effect of hot water extracts of fresh and dried gingers on HRSV was tested by plaque reduction assay in both human upper (HEp-2) and low (A549) respiratory tract cell lines. Ability of ginger to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA). Results: Fresh ginger dose-dependently inhibited HRSV-induced plaque formation in both HEp-2 and A549 cell lines (p<0.0001). In contrast, dried ginger didn't show any dose-dependent inhibition. 300 μg/ml fresh ginger could decrease the plaque counts to 19.7% (A549) and 27.0% (HEp-2) of that of the control group. Fresh ginger was more effective when given before viral inoculation (p<0.0001), particularly on A549 cells. 300 μg/ml fresh ginger could decrease the plaque formation to 12.9% when given before viral inoculation. Fresh ginger dose-dependently inhibited viral attachment (p<0.0001) and internalization (p<0.0001). Fresh ginger of high concentration could stimulate mucosal cells to secrete IFN-β that possibly contributed to counteracting viral infection. Conclusions: Fresh, but not dried, ginger is effective against HRSV-induced plaque formation on airway epithelium by blocking viral attachment and internalization. © 2012 Elsevier Ireland Ltd.
Tseng S.-P.,Tajen University
2016 2nd IEEE International Conference on Computer and Communications, ICCC 2016 - Proceedings | Year: 2017
Harmony search(HS) is a music-inspired metaheuristic for optimization problem. HS is simple to implement and apply on several kinds of problems. But HS is not successful to solve large size of travelling salesman problem(TSP). In this paper, we try to find the difficulties of HS on TSP. And we propose the elitists harmony search(EHS) to overcome these difficulties. The EHS reorganizes the harmony memory, which is divided into two groups: elitists and explorers. Elitists are used to raise the intensification and explorers are for the diversification. The experimental result shows that EHS is better than HS average 25.49%, and EIHS is better than IHS average 20.09%. The difference of execution time is very small. And for larger TSP benchmark, the EHS is more better than HS. © 2016 IEEE.
Chakraborty C.,Galgotias University |
Doss C.G.P.,Vellore Institute of Technology |
Bandyopadhyay S.,Indian Statistical Institute |
Agoramoorthy G.,Tajen University
Wiley Interdisciplinary Reviews: RNA | Year: 2014
The prevalence of type-2 diabetes (T2D) is increasing significantly throughout the globe since the last decade. This heterogeneous and multifactorial disease, also known as insulin resistance, is caused by the disruption of the insulin signaling pathway. In this review, we discuss the existence of various miRNAs involved in regulating the main protein cascades in the insulin signaling pathway that affect insulin resistance. The influence of miRNAs (miR-7, miR-124a, miR-9, miR-96, miR-15a/b, miR-34a, miR-195, miR-376, miR-103, miR-107, and miR-146) in insulin secretion and beta (β) cell development has been well discussed. Here, we highlight the role of miRNAs in different significant protein cascades within the insulin signaling pathway such as miR-320, miR-383, miR-181b with IGF-1, and its receptor (IGF1R); miR-128a, miR-96, miR-126 with insulin receptor substrate (IRS) proteins; miR-29, miR-384-5p, miR-1 with phosphatidylinositol 3-kinase (PI3K); miR-143, miR-145, miR-29, miR-383, miR-33a/b miR-21 with AKT/protein kinase B (PKB) and miR-133a/b, miR-223, miR-143 with glucose transporter 4 (GLUT4). Insulin resistance, obesity, and hyperlipidemia (high lipid levels in the blood) have a strong connection with T2D and several miRNAs influence these clinical outcomes such as miR-143, miR-103, and miR-107, miR-29a, and miR-27b. We also corroborate from previous evidence how these interactions are related to insulin resistance and T2D. The insights highlighted in this review will provide a better understanding on the impact of miRNA in the insulin signaling pathway and insulin resistance-associated diagnostics and therapeutics for T2D. © 2014 John Wiley & Sons, Ltd.
Tzeng T.-F.,Pao Chien Hospital |
Liu I.-M.,Tajen University
Phytomedicine | Year: 2013
6-Gingerol ((S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone) is one of the pungent constituents of Zingiber zerumbet (L) Smith (Zingiberaceae family). In this study, we investigated the effects of 6-gingerol on the inhibition of adipogenesis in 3T3-L1 cells. After treatment with 6-gingerol in differentiation medium for 4 or 8 days, the 3T3-L1 cells were lysed for experimental analysis. Cells were stained with Oil-Red-O to detect oil droplets in adipocytes. The 3T3-L1 cells were lysed and measured for triglyceride contents. The protein expression of adipogenesis-related transcription factor was evaluated by Western blot analysis. 6-Gingerol suppressed oil droplet accumulation and reduced the droplet size in a concentration (5-15 μg/ml)- and time-dependent manner. Treatment of 3T3-L1 cells with 6-gingerol reduced the protein levels of peroxisome proliferator-activated receptor (PPAR)γ and CCAAT/enhancer-binding protein (C/EBP)α. Additionally, the protein levels of fatty acid synthase (FAS) and adipocyte-specific fatty acid binding protein (aP2) decreased upon treatment with 6-gingerol. Meanwhile, 6-gingerol diminished the insulin-stimulated serine phosphorylation of Akt (Ser473) and GSK3β (Ser9). These results suggest that 6-gingerol effectively suppresses adipogenesis and that it exerts its role mainly through the significant down-regulation of PPARγ and C/EBPα and subsequently inhibits FAS and aP2 expression. 6-Gingerol also inhibited differentiation in 3T3-L1 cells by attenuating the Akt/GSK3β pathway. Our findings provide important insights into the mechanisms underlying the anti-adipogenic activity of 6-gingerol. © 2013 Elsevier GmbH. All rights reserved.
Wang Z.,Tajen University
MATEC Web of Conferences | Year: 2016
Tourism industry is closely linked with the natural environment but with a highly indivisibility of symbiotic relationship. Green tourism and green tourism hotel are not only the spindle stage of development industry. The environmental protection is also an environmental conservation and sustainable development of substantive liability demonstration. The study is also belong to the substance RDF itself, so we can call "clean energy". The raw materials came from agricultural waste through proper blending ratio and control technology, after PP14 adhesive extruded through the fluidized bed pyrolysis cracking process to burn stability. The recovery can also be used as fuel volatile process of drying and gasification. However, in the actual economic cost of the test running the hotel industry can reduce the cost per MJ USD $ 0.0082, more economical than coal expenses 23.17% of the fuel. Therefore, green hotel through biomass fuels RDF as clean fuels can further reduce carbon emissions to reach the green hotel of expectations. © The Authors, published by EDP Sciences, 2016.
Chakraborty C.,Iilm Academy Of Higher Learning Greater Noida |
Agoramoorthy G.,Tajen University
Biotechnology Advances | Year: 2010
India's biotechnology industry has been growing towards new heights in conjunction with the recent economic outburst. The country has the potential to revolutionize biopharmaceutical and healthcare sectors. In this review, we have highlighted the achievements of India's biotechnology industry, especially biopharmaceutical and healthcare sectors that include therapeutics, diagnostics, stem cell research, human healthcare related bioinformatics and animal health care. We have also described regulatory mechanisms involved in India's health care biotech including manpower development. © 2009 Elsevier Inc. All rights reserved.
Chinnasamy P.,International Water Management Institute |
Agoramoorthy G.,Tajen University
Water Resources Management | Year: 2015
Groundwater irrigation is the most predominant method used across India. The south Indian state of Tamil Nadu is one of the largest producers of agricultural products, and is largely dependent on groundwater for irrigation. The impact of increased irrigation due to intensification of agricultural activities on groundwater levels has not been well researched, both spatially and temporally. Hence this study has used remote sensing data from NASA’s Gravity Recovery and Climate Experiment and the Global Land Data Assimilation Systems to assess the total change in groundwater storage across the state of Tamil Nadu over a period of 11 years, from 2002 to 2012. The results show groundwater depletion at the rate of 21.4 km3 yr−1, which is 8% more than the annual recharge rate (19.81 km3 yr−1) owing to the total rainfall of 1016 mm yr1. Maximum depletion was observed in 2008, while the least depletion was observed in 2002 with rates of 41.15 and 0.32 cm yr−1, respectively. Districts such as Dharmapuri, Vellore and Thiruvannamali encountered intense groundwater depletion. Observed spatiotemporal groundwater storage will benefit India’s water resource managers and policymakers for the future management of groundwater resources to enhance food and water security. © 2015, Springer Science+Business Media Dordrecht.
Chakraborty C.,Vellore Institute of Technology |
Agoramoorthy G.,Tajen University |
Hsu M.J.,National Sun Yat - sen University
PLoS ONE | Year: 2011
Insulin receptor substrate (IRS) harbors proteins such as IRS1, IRS2, IRS3, IRS4, IRS5 and IRS6. These key proteins act as vital downstream regulators in the insulin signaling pathway. However, little is known about the evolutionary relationship among the IRS family members. This study explores the potential to depict the evolutionary relationship among the IRS family using bioinformatics, algorithm analysis and mathematical models. © 2011 Chakraborty et al.