Taizhou Peoples Hospital Taizhou

Taizhou, China

Taizhou Peoples Hospital Taizhou

Taizhou, China
SEARCH FILTERS
Time filter
Source Type

PubMed | The Institute of CMC Biobank and Translational Medicine Taizhou, Taizhou Peoples Hospital Taizhou and Tongji University
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2014

Unc-51-Like Kinase 1 (ULK1) is regarded as a central role in autophagy. Although the details of how ULK1 triggers autophagy are obscure, the relationship between ULK1 expression and the diagnosis and prognosis of cancer patients may guide the clinical practice and scientific research. The aim of this study was to investigate and compare the expression level of ULK1 in 86 paired esophageal squamous cell carcinoma (ESCC) and paracancerous tissues, and to examine the effect of ULK1 expression on the prognosis of ESCC patients. ULK1 was primarily expressed in cytoplasm, but was rarely seen in nucleus. The levels of cytoplasmic ULK1 in ESCC tissues were higher than those in paracancerous tissue (P < 0.01) and significantly associated with lymph node metastasis (LNM) (P = 0.025). Survival analysis showed that patients with low expression of cytoplasmic ULK1 had worse survival time than those with high expression of cytoplasmic ULK1 (hazard ratio = 1.754, 95% confidence interval: 1.022-3.010, P = 0.041), which disappeared after adjustment for TNM stages and LNM (P = 0.319). In conclusion, ULK1 might play an important role in the occurrence and development of ESCC and represent a potential prognostic biomarker for ESCC patients. However, the precise impact of ULK1 on predicting the prognosis of patients with ESCC requires further investigation.


Han G.,Suzhou University | Tian Y.,Suzhou University | Duan B.,Tongji University | Sheng H.,National Engineering Center for Biochip at Shanghai Shanghai | And 2 more authors.
International journal of clinical and experimental pathology | Year: 2014

Although recent progress has been made in the diagnosis and treatment of cancer, the prognosis of esophageal squamous cell carcinoma (ESCC) remains poor. The identification of biomarkers for ESCC prognosis is important for treatment decisions. The aim of this study was to evaluate the relationship between the expressions of Annexin A1 (ANXA1), three prime repair exonuclease 1 (TREX1) and apurinic/apyrimidinic endonuclease-1 (APE1) and clinical outcome of patients with ESCC. The expressions of ANXA1, TREX1 and APE1 in 93 pairs of ESCC and paracancerous tissues were tested using immunohistochemistry. ANX1, TREX1 and APE1 were dysregulated in ESCC. Nuclear expressions of ANXA1 and APE1 were significantly associated with pathologic type (P = 0.004 and 0.040, respectively). Patients with low expression of nuclear ANXA1 had a better prognosis than those with high expression of nuclear ANXA1 (HR = 0. 448, 95% CI 0.236-0.849, P = 0.014), especially for those with histologic grade 1 and 2 (HR = 0.303, 95% CI: 0.155-0.593, P < 0.001). In conclusion, nuclear ANXA1 may be potentially used as a prognostic biomarker for ESCC.


PubMed | Taizhou Peoples Hospital Taizhou, Tongji University and CMC Biobank and Translational Medicine Institute Taizhou
Type: Journal Article | Journal: American journal of translational research | Year: 2013

MicroRNA-200c (miR-200c) influences sensitivity to chemotherapy and radiotherapy in vitro. This study was designed to investigate the prognostic potential of serum miR-200c in patients with advanced esophageal squamous cancer (ESCC). The serum levels of miR-200c was assayed by quantitative RT-PCR in 157 healthy subjects and 157 patients with advanced ESCC who were treated with platinum-based chemotherapy. The serum levels of miR-200c in advanced ESCC patients was significantly increased compared with those in controls (P < 0.001). Serum miR-200c expression was significantly associated with TNM stage (P = 0.037) and treatment response (P = 0.021). Patients with high expression of serum miR-200c had a higher risk for death than those with low expression of serum miR-200c (adjusted hazard ratios = 1.665, 95% confidence intervals: 1.135-2.443, P = 0.009). In conclusion, serum miR-200c may serve as predictor of survival for advanced ESCC and provide information for personalized therapy in advanced ESCC.


PubMed | Taizhou Peoples Hospital Taizhou
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

Angiopoietin-like protein 3 (ANGPTL3) plays an important role in angiogenesis. This study aimed to examine the protein expression of ANGPTL3, and to evaluate its clinical significance in esophageal cancer. ANGPTL3 expression was detected using immunohistochemistry in 98 pairs of esophageal cancer and adjacent non-cancerous tissues. The expression levels of ANGPTL3 in esophageal cancer tissues were significantly higher than those in adjacent noncancerous tissues (P < 0.05). No association was observed between ANGPTL3 expression and clinical features (P > 0.05). Although ANGPTL3-negative patients had longer survival time than ANGPTL3-positive patients, the difference did not reach statistical significance (P = 0.090). Stratified analysis of ANGPTL3 expression according to clinical features revealed that there was significant association between ANGPTL3 expression and overall survival among patients aged 65 years or younger, female, or with lymph node metastasis (P < 0.05). When after adjusted for clinical features, the association remained significant only in patients aged 65 years or younger (P = 0.021).Taken together, our findings provide preliminary evidence of association of ANGPTL3 expression with the prognosis of subgroups of patients with esophageal cancer.


PubMed | Chinese Institute of Clinical Medicine and Taizhou Peoples Hospital Taizhou
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2015

To investigate the expression profiles of long non-coding RNAs (lncRNAs) in atrial fibrillation (AF), atrial tissues from 3 AF patients and 3 non-AF patients that were collected for lncRNA expression microarray analyses to explore the role of lncRNA in the pathogenesis of AF. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to identify the main functions of the differentially expressed genes and AF-related pathways. A total of 219 lncRNAs was found to be differentially expressed between AFs and controls. Among them, 156 were upregulated and 63 were downregulated. Eight out of 10 dysregulated lncRNAs such as uc001eqh.1 were validated by quantitative real-time PCR. GO categories, pathway analyses, and interaction network showed a consistent result that differentially expressed genes contribute to the pathogenesis of AF. In conclusion, the findings of our study provide a perspective on lncRNA in AF and the foundation for further study of the biological functions of lncRNAs in AF.


PubMed | Taizhou Peoples Hospital Taizhou, Second Peoples Hospital of Lian Yungang Lian Yungang and Nanjing Medical University
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2016

IGF-I CA repeat polymorphisms, especially the allele containing CA19 repeats, have been reported to be associated with the risk for various types of cancers. However, the results still remain controversial and ambiguous. This meta-analysis was performed to evaluate the association between IGF-I CA19 repeat polymorphisms and the risk of cancer. Total 18 studies with IGF-I CA19 repeat genotyping on 9,873 patients and 15,607 controls were analyzed. We used random-effects model with a pooled OR of 0.69 (95% CI = 0.60-0.79) for the recessive genetic model, 0.97 (95% CI = 0.86-1.10) for the dominant genetic model, 0.99 (95% CI = 0.86-1.14) for the homozygote comparison and 1.06 (95% CI = 0.91-1.23) for the heterozygote comparison. In the subgroup analysis of recessive model, OR (95% CI) was 0.65 (0.52-0.80) in breast cancer, 0.68 (0.53-0.86) in prostate cancer, and 0.71 (0.52-0.96) in Caucasian. In conclusion, IGF-1 CA19 repeat polymorphisms are unlikely to be a major determinant of susceptibility to cancer. However, the subgroup analysis of recessive model indicates that IGF-I CA19 repeat polymorphisms may reduce the risk of certain types of cancer or in a specific population.


PubMed | Fudan University, Taizhou Peoples Hospital Taizhou and Taizhou Central Hospital Taizhou
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2014

MicroRNA-218 (miR-218) acts as a tumor suppressor in numerous types of cancer by regulation of the expression of target genes. The aim of this study was to investigate whether polymorphisms in miR-218 LAMB3 pathway were associated with the risk and prognosis of esophageal squamous cell carcinoma (ESCC). Pri-mir-218 rs11134527 and LAMB3 rs2566 were genotyped in ESCC patients and 745 controls to assess their associations with cancer risk and overall survival. Pri-mir-218 rs11134527 was significantly associated with a decreased risk of ESCC under codominant, recessive and additive models. Although there was a significant association between rs11134527 and better survival of ESCC patients under codominant, recessive and additive models, the association disappeared after adjustment for TNM and LNM. However, further stratified analysis revealed that the association remained significant in patients with TNM stages I and II or non-LNM. Our data suggest that pri-miR-218 rs11134527 may contribute to the genetic susceptibility and prognosis for ESCC in Chinese Han population.


PubMed | National Engineering Center for Biochip at Shanghai Shanghai, Tongji University, Suzhou University and Taizhou Peoples Hospital Taizhou
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2014

Although recent progress has been made in the diagnosis and treatment of cancer, the prognosis of esophageal squamous cell carcinoma (ESCC) remains poor. The identification of biomarkers for ESCC prognosis is important for treatment decisions. The aim of this study was to evaluate the relationship between the expressions of Annexin A1 (ANXA1), three prime repair exonuclease 1 (TREX1) and apurinic/apyrimidinic endonuclease-1 (APE1) and clinical outcome of patients with ESCC. The expressions of ANXA1, TREX1 and APE1 in 93 pairs of ESCC and paracancerous tissues were tested using immunohistochemistry. ANX1, TREX1 and APE1 were dysregulated in ESCC. Nuclear expressions of ANXA1 and APE1 were significantly associated with pathologic type (P = 0.004 and 0.040, respectively). Patients with low expression of nuclear ANXA1 had a better prognosis than those with high expression of nuclear ANXA1 (HR = 0. 448, 95% CI 0.236-0.849, P = 0.014), especially for those with histologic grade 1 and 2 (HR = 0.303, 95% CI: 0.155-0.593, P < 0.001). In conclusion, nuclear ANXA1 may be potentially used as a prognostic biomarker for ESCC.


PubMed | Taizhou Peoples Hospital Taizhou, Suzhou University, National Engineering Center for Biochip Shanghai and Tongji University
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

Pancreatic cancer (PC) has the poorest survival rate among all types of human cancer due to the lack of sensitive and non-invasive diagnostic screen methods for PC screening. Our aim was to identify novel serum microRNA (miRNA) biomarkers for the early detection of PC. We used microarray to screen differential expression of miRNAs in two pooled serum samples (6 PC patients and 6 healthy controls). A panel of miRNAs (22 over-expression and 23 decreased) were deregulated in serum of PC patients in comparison to controls. The expressions of 8 selected miRNAs were further evaluated in sera from 49 PC patients and 27 controls using quantitative reverse transcription-polymerase chain reaction. The levels of serum miR-492 and miR-663a were significantly decreased in PC patients compared with controls (P < 0.05). ROC curve analysis showed that serum miR-492 and miR-663a yield an AUC of 0.787 with 75.5% sensitivity and 70.0% specificity and 0.870 with 85.7% sensitivity and 80.0% specificity, respectively, for discriminating between PC patients and healthy controls. In addition, the level of miR-663a was significantly and inversely associated with TNM stage (P = 0.027). These results suggested that serum miR-492 and miR-663a could have strong potential as novel non-invasive biomarkers for the early detection of PC.


PubMed | Chinese Institute of Clinical Medicine, Yangzhou University, Tongji University, Taizhou Peoples Hospital Taizhou and Jingjiang Peoples Hospital Taizhou
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2016

Guanine nucleotide binding protein-like 3 (GNL3) is a GIP-binding nuclear protein that has been reported to be involved in various biological processes, including cell proliferation, cellular senescence and tumorigenesis. This study aimed to investigate the expression level of GNL3 in gastric cancer and to evaluate the relationship between its expression and clinical variables and overall survival of gastric cancer patients. The expression level of GNL3 was examined in 89 human gastric cancer samples using immunohistochemistry (IHC) staining. GNL3 in gastric cancer tissues was significantly upregulated compared with paracancerous tissues. GNL3 expression in adjacent non-cancerous tissues was associated with sex and tumor size. Survival analyses showed that GNL3 expression in both gastric cancer and adjacent non-cancerous tissues were not related to overall survival. However, in the subgroup of patients with larger tumor size ( 6 cm), a close association was found between GNL3 expression in gastric cancer tissues and overall survival. GNL3-positive patients had a shorter survival than GNL3-negative patients. Our study suggests that GNL3 might play an important role in the progression of gastric cancer and serve as a biomarker for poor prognosis in gastric cancer patients.

Loading Taizhou Peoples Hospital Taizhou collaborators
Loading Taizhou Peoples Hospital Taizhou collaborators