Dong L.,Jiangsu Taixing Peoples Hospital |
Dong L.,Nantong University |
Zhang Y.,Nantong University |
Bao Y.,Nantong University |
Zhang W.,Nantong University
Pharmaceutical Care and Research | Year: 2013
Objective: To investigate the effect of 2,3,4′,5- tetrahydroxystilbene-2-O-β-D-glucoside (TSG) on the expression of insulin-like growth factor-1 (IGF-1) in myocardial tissue of diabetes rats. Methods: Diabetes model rats was developed by feeding a high-fat diet and single intraperitoneal injection of 1% streptozocin (STZ, 30 mg/kg). Forty STZ-diabetic rats were randomly divided into 4 groups: the diabetes model, the low-, middle- and high-dose TSG groups. In the low-, middle- and high-dose groups, rats were administered TSG 30, 60 and 120 mg/kg by gavage once daily for 6 weeks. Another 10 rats were used as controls. Body weight, food and water consumptions were collected and fasting blood glucose (FBG) was determined. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the content of serum IGF-1 and immunofluorescence was applied to determine the expression of IGF-1 in myocardial tissue. Results: The content of serum IGF-1 and the expression of myocardial IGF-1 in diabetes model rats were decreased (P<0.05, P<0.01) when compared with the controls. The content of serum IGF-1 and the expression of myocardial IGF-1 in the middle- and high-dose TSG groups were increased (P<0.05, P<0.01) when compared with diabetes model rats. Conclusion: TSG could increase serum IGF-1 level, up-regulate the expression of myocardial IGF-1.