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Wang H.-C.,Kaohsiung Medical University | Chu F.-H.,National Taiwan University | Chien S.-C.,National Chung Hsing University | Liao J.-W.,National Chung Hsing University | And 8 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Antrodia cinnamomea is an edible fungus endemic to Taiwan that has been attributed with health promotion benefits. An A. cinnamomea mycelium health food product, which was produced by solid-state culture, was selected as the target for investigation in this study. Fourteen representative metabolites of A. cinnamomea mycelium (EMAC) were selected as index compounds to establish the metabolite profile for evaluation of EMAC product quality. It was also demonstrated that EMAC administration significantly reduced liver inflammation and serum oxidative stress in vivo. 4-Acetylantroquinonol B obtained by a bioactivity-guided fractionation from EMAC was able to not only inhibit LPS-induced nitric oxide formation in macrophages but also protect against ethanol-induced oxidative stress in liver cells. The results suggest this A. cinnamomea product might be a potent antioxidative and anti-inflammatory supplement for chemoprevention. © 2013 American Chemical Society.


Lin T.-Y.,National Chung Hsing University | Chen C.-Y.,National Chung Hsing University | Chen C.-Y.,National Taiwan University | Chien S.-C.,National Chung Hsing University | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011

Antrodia cinnamomea is a precious edible fungus endemic to Taiwan that has long been used as a folk remedy for health promotion and for treating various diseases. In this study, an index of 13 representative metabolites from the ethanol extract of A. cinnamomea fruiting body was established for use in quality evaluation. Most of the index compounds selected, particularly the ergostane-type triterpenoids and polyacetylenes, possess good anti-inflammation activity. A comparison of the metabolite profiles of different ethanol extracts from A. cinnamomea strains showed silmilar metabolites when the strains were grown on the original host wood (Cinnamomum kanehirai) and harvested after the same culture time period (9 months). Furthermore, the amounts of typical ergostane-type triterpenoids in A. cinnamomea increased with culture age. Culture substrates also influenced metabolite synthesis; with the same culture age, A. cinnamomea grown on the original host wood produced a richer array of metabolites than A. cinnamomea cultured on other wood species. We conclude that analysis of a fixed group of compounds including triterpenoids, benzolics, and polyacetylenes constitutes a suitable, reliable system to evaluate the quality of ethanol extract from A. cinnamomea fruiting bodies. The evaluation system established in this study may provide a platform for analysis of the products of A. cinnamomea. © 2011 American Chemical Society.


Lin C.-C.,Taiwan Leader Biotech Company | Kumar K.J.S.,National Chung Hsing University | Liao J.-W.,National Chung Hsing University | Kuo Y.-H.,China Medical University at Taichung | And 3 more authors.
Toxicology Reports | Year: 2015

Antrodia cinnamomea is a rare and endemic medicinal mushroom native to Taiwan. The pharmacological effects of A. cinnamomea have been extensively studied. The aim of the present study was to assess the genotoxic, oral toxic and teratotoxic effects of A. cinnamomea health food product "Leader Deluxe Antrodia cinnamomea (LDAC)'' using in vitro and in vivo tests. The Ames test with 5 strains of Salmonella typhimurium showed no signs of increased reverse mutation upon exposure to LDAC up to concentration of 5. mg/plate. Exposure of Chinese Hamster Ovary cells (CHO-K1) to LDAC did not produce an increase in the frequency of chromosomal aberration in vitro. In addition, LDAC treatment did not affect the proportions of immature to total erythrocytes and the number of micronuclei in the immature erythrocytes of ICR mice. Moreover, 14-days single-dose acute toxicity and 90-days repeated oral dose toxicity tests with rats showed that no observable adverse effects were found. Furthermore, after treatment with LDAC (700-2800. mg/kg/day) there was no evidence of observable segment II reproductive and developmental toxic effects in pregnant SD rats and their fetuses. These toxicological assessments support the safety of LDAC for human consumption. © 2015 The Authors.


Gokila Vani M.,National Chung Hsing University | Kumar K.J.S.,China Medical University at Taichung | Liao J.-W.,National Chung Hsing University | Chien S.-C.,National Chung Hsing University | And 7 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013

In this study, we investigated the cytoprotective effects of antcin C, a steroid-like compound isolated from Antrodia cinnamaomea against AAPH-induced oxidative stress and apoptosis in human hepatic HepG2 cells. Pretreatment with antcin C significantly protects hepatic cells from AAPH-induced cell death through the inhibition of ROS generation. Furthermore, AAPH-induced lipid peroxidation, ALT/AST secretion and GSH depletion was significantly inhibited by antcin C. The antioxidant potential of antcin C was correlated with induction of antioxidant genes including, HO-1, NQO-1, γ-GCLC, and SOD via transcriptional activation of Nrf2. The Nrf2 activation by antcin C is mediated by JNK1/2 and PI3K activation, whereas pharmacologic inhibition of JNK1/2 and PI3K abolished antcin C-induced Nrf2 activity. In addition, AAPH-induced apoptosis was significantly inhibited by antcin C through the down-regulation of pro-apoptotic factors including, Bax, cytochrome c, capase 9, -4, -12, -3, and PARP. In vivo studies also show that antcin C significantly protected mice liver from AAPH-induced hepatic injury as evidenced by reduction in hepatic enzymes in circulation. Further, immunocytochemistry analyses showed that antcin C significantly increased HO-1 and Nrf2 expression in mice liver tissues. These results strongly suggest that antcin C could protect liver cells from oxidative stress and cell death via Nrf2/ARE activation. © 2013 M. Gokila Vani et al.


PubMed | Taiwan Leader Biotech Company, National Taiwan University and National Chung Hsing University
Type: Journal Article | Journal: Oncotarget | Year: 2016

The present study revealed the anti-aging properties of antcin M (ANM) and elucidated the molecular mechanism underlying the effects. We found that exposure of human normal dermal fibroblasts (HNDFs) to high-glucose (HG, 30 mM) for 3 days, accelerated G0/G1 phase arrest and senescence. Indeed, co-treatment with ANM (10 M) significantly attenuated HG-induced growth arrest and promoted cell proliferation. Further molecular analysis revealed that ANM blocked the HG-induced reduction in G1-S transition regulatory proteins such as cyclin D, cyclin E, CDK4, CDK6, CDK2 and protein retinoblastoma (pRb). In addition, treatment with ANM eliminated HG-induced reactive oxygen species (ROS) through the induction of anti-oxidant genes, HO-1 and NQO-1 via transcriptional activation of Nrf2. Moreover, treatment with ANM abolished HG-induced SIPS as evidenced by reduced senescence-associated -galactosidase (SA--gal) activity. This effect was further confirmed by reduction in senescence-associated marker proteins including, p21CIP1, p16INK4A, and p53/FoxO1 acetylation. Also, the HG-induced decline in aging-related marker protein SMP30 was rescued by ANM. Furthermore, treatment with ANM increased SIRT-1 expression, and prevented SIRT-1 depletion. This protection was consistent with inhibition of SIRT-1 phosphorylation at Ser47 followed by blocking its upstream kinases, p38 MAPK and JNK/SAPK. Further analysis revealed that ANM partially protected HG-induced senescence in SIRT-1 silenced cells. A similar effect was also observed in Nrf2 silenced cells. However, a complete loss of protection was observed in both Nrf2 and SIRT-1 knockdown cells suggesting that both induction of Nrf2-mediated anti-oxidant defense and SIRT-1-mediated deacetylation activity contribute to the anti-aging properties of ANM in vitro. Result of in vivo studies shows that ANM-treated C. elegens exhibits an increased survival rate during HG-induced oxidative stress insult. Furthermore, ANM significantly extended the life span of C. elegans. Taken together, our results suggest the potential application of ANM in age-related diseases or as a preventive reagent against aging process.


A solid-state fermentation method is provided. The method is provided for edible and medicinal microorganisms. A commensurate device is used for processing sterilization, inoculation, cultivation, drying, and collection. Cultivation substrates are filled in cultivation plates and placed on trays of a movable layer rack in a reaction fermenter for fermentation. Inner pipes provide inoculation, fluid nutrient, and air for fermentation of microorganisms. The reaction fermenter is accompanied with a dry circulation unit and a production collection unit for drying and collection. The solid-state fermentation processes are thus effectively integrated without the need of transferring fermented products between stations, which largely reduces contamination rate. Even more, there is no need to purchase automatic production equipments of high mechanical technologies. Thus, the present invention reduces labor requirement, saves time and energy, deducts cost of automatic equipments, achieves low contamination rate, and processes automatic mass production even without automatic production equipments.


PubMed | China Medical University at Taichung, Taiwan Leader Biotech Company and National Chung Hsing University
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2014

In this study, we investigated the cytoprotective effects of antcin C, a steroid-like compound isolated from Antrodia cinnamaomea against AAPH-induced oxidative stress and apoptosis in human hepatic HepG2 cells. Pretreatment with antcin C significantly protects hepatic cells from AAPH-induced cell death through the inhibition of ROS generation. Furthermore, AAPH-induced lipid peroxidation, ALT/AST secretion and GSH depletion was significantly inhibited by antcin C. The antioxidant potential of antcin C was correlated with induction of antioxidant genes including, HO-1, NQO-1, -GCLC, and SOD via transcriptional activation of Nrf2. The Nrf2 activation by antcin C is mediated by JNK1/2 and PI3K activation, whereas pharmacologic inhibition of JNK1/2 and PI3K abolished antcin C-induced Nrf2 activity. In addition, AAPH-induced apoptosis was significantly inhibited by antcin C through the down-regulation of pro-apoptotic factors including, Bax, cytochrome c, capase 9, -4, -12, -3, and PARP. In vivo studies also show that antcin C significantly protected mice liver from AAPH-induced hepatic injury as evidenced by reduction in hepatic enzymes in circulation. Further, immunocytochemistry analyses showed that antcin C significantly increased HO-1 and Nrf2 expression in mice liver tissues. These results strongly suggest that antcin C could protect liver cells from oxidative stress and cell death via Nrf2/ARE activation.


Patent
Taiwan Leader Biotech Corporation | Date: 2010-02-23

The present invention is related to the usage of Antrodia cinnamomea fruit body derivatives. The fruit body derivatives comprise acetic acid extract, acetic ether extract and Antrocamphin A. The acetic acid extract, acetic ether extract and Antrocamphin A can reduce the expression of iNOS and COX-2 and therefore inhibit the production of pro-inflammatory molecules.


Patent
National Chung Hsing University and Taiwan Leader Biotech Corporation | Date: 2011-12-12

A polyacetylenes and application thereof. The polyacetylenes is isolated from an extract of the sporophores of Antrodia Cinnamomea and has a function of inhibiting the production of nitric oxide. Therefore, the polyacetylenes can be used for preparing a pharmaceutical composition for anti-inflammation. The present invention also teaches the representative metabolites of the sporophores of Antrodia Cinnamomea, which can be used to evaluate the quality thereof.


Le

Trademark
TAIWAN LEADER BIOTECH Corporation | Date: 2014-04-24

Medicinal herbs; Medicinal herbal extracts for medical purposes; Medicinal preparations for the mouth to be applied in the form of drops, capsules, tablets and compressed tablets; Drug delivery agents in the form of capsules that provide controlled release of the active ingredients for a wide variety of pharmaceuticals; Nutritional food additives for medical purposes in the nature of natural food extracts derived from fish, meat, vegetables; Edible fish oils for medical purposes. Export and import agencies; Goods or services price quotations; On-line auctioneering services via the Internet; Auction services; Retail department store services; Supermarkets; Retail convenience stores; Provision of information and advice to consumers regarding the selection of products and items to be purchased; Wholesale store services featuring agricultural products, cleaning preparations, drugs, medicinal herbs, stationery, livestock products, aquatic products, food.

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