Taishan Medicine College

Taian, China

Taishan Medicine College

Taian, China
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Li Y.,Shantou University | Li Y.,Taishan Medicine College | Xu N.,National Detergent and Cosmetics Products Quality Supervision and Inspection Center Guangdong | Pan J.,Shantou University | And 3 more authors.
Sensors and Actuators, B: Chemical | Year: 2017

In the present study, the authors report a novel sandwich biosensor for detection of thrombin using electrochemiluminescence method based on dual-signal amplification strategy. Firstly, the gold nanoparticles graphene composite (GNPs-graphene) was obtained by simultaneously depositing the chloroauric (III) acid tetrahydrate [HAuCl4·4H2O] and graphene solution with a cyclic voltammetry. Secondly, one thiol-modified thrombin aptamer (TBA1) was immobilized the glassy carbon electrode (GCE) via Au-S bonding. And then the other thiol-modified biotinylated thrombin aptamer (TBA2) that were modified with Ru(bpy)3 2+ were anchored on the surface of the gold nanoparticles in solution. This proposed assay took dual signal amplification strategies. On the one hand, a single gold nanoparticle can connect many TBA2 to come into being a signal cluster (Ru-TBA2-AuNPs), thus when a little thrombin were present the system the ECL intensity will have a strong signal response; On the other hand, the GNPs-graphene can promote the electron transfer efficiency and increase the signal response. The ECL signal had a good linear relationship with thrombin concentration in range of 0.01–10 nM, and the detection limit for thrombin was determined to be as low as 6.3 pM. This biosensor also showed good selectivity for thrombin without being affected by some other proteins, such as BSA and lysozyme and so on. Moreover, this proposed method was successfully applied to thrombin analysis in diluted human serum samples. © 2016


Xia C.-C.,Taishan Medicine College
Acta Crystallographica Section E: Structure Reports Online | Year: 2010

In the title compound, C 11H 13NO 4, the two fused rings are almost coplanar, making a dihedral angle of 3.02 (8)°. In the crystal, chains are formed parallel to [010] through N - H⋯O hydrogen bonds between the amine and carbonyl groups.


Huang X.,Shantou University | Li Y.,Taishan Medicine College | Chen Y.,Shantou University | Gao W.,Shantou University
RSC Advances | Year: 2015

An ingenious sensing strategy for detecting thrombin in human serum has been developed on the basis of a hairpin DNA sequence and resonance light scattering (RLS) technique. A thrombin aptamer sequence was embedded inside the hairpin DNA strand (H-eTBA), which was designed to be the loop-stem structure. Moreover, methylene blue (MB) was utilized as the RLS signal indicator according to its different affinity to single or double stranded DNA. Upon the addition of thrombin, the thrombin aptamer inside H-eTBA interacted specifically with thrombin. Thus the conformation of H-eTBA would change. After the introduction of the DNA strand (CTBA), which was complementary to H-eTBA, the amount of double stranded DNA would decrease as a consequence. Later when MB solution was added, the RLS signal would present various response values based on different amounts of thrombin. The determination of thrombin in human serum could be obtained with a detection limit of 0.32 nM and this specific sensor could be applied to detect thrombin practically. Furthermore, this aptasensor showed quite good selectivity and simplicity toward thrombin. Finally, the proposed sensing method showed its superiority with selectivity and practicability, which could be used as a simple platform for thrombin detection. © 2015 The Royal Society of Chemistry.


Huang X.,Shantou University | Li Y.,Taishan Medicine College | Zhang X.,Shantou University | Chen Y.,Shantou University | Gao W.,Shantou University
Analyst | Year: 2015

An efficient aptasensor was developed in which graphene oxide (GO) was employed as an indicator for both electrochemical impedance spectroscopy and electrochemiluminescence (ECL) signal generation. The aptasensor was fabricated by self-assembling the ECL probe of a thiolated adenosine triphosphate binding aptamer (ABA) tagged with a Ru complex (Ru(bpy)32+ derivatives) onto the surface of gold nanoparticle (AuNP) modified glassy carbon electrode (GCE). ABA immobilized onto AuNP modified GCE could strongly adsorb GO due to the strong π-π interaction between ABA and graphene oxide; ECL quenching of the Ru complex then takes place because of energy transfer and electron transfer, and a large increase of the electron transfer resistance (Ret) of the electrode. While in the presence of target adenosine triphosphate (ATP), the ABA prefers to form ABA-ATP bioaffinity complexes, which have weak affinity to graphene oxide and keep the graphene oxide away from the electrode surface, thus allowing the ECL signal enhancement, and in conjunction with the decrease of the Ret. Because of the high ECL quenching efficiency, unique structure, and electronic properties of graphene oxide, the Ret and ECL intensity versus the logarithm of ATP concentration was linear in the wide range from 10 pM to 10 nM with an ultra-low detection limit of 6.7 pM to 4.8 pM, respectively. The proposed aptasensor exhibited excellent reproducibility, stability, and outstanding selectivity, and ATP could be effectively distinguished from its analogues. More significantly, this efficient ECL aptasensor strategy based on GO acting both as an electrochemical and ECL signal indicator is general and can be easily extended to other biological binding events. This journal is © The Royal Society of Chemistry.


Li Y.,Shantou University | Li Y.,Taishan Medicine College | Wu Y.,Shantou University | Lu F.,Shantou University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2016

Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier. Firstly, the GNPs and graphene were simultaneously deposited on the glassy carbon electrode (GCE) by cyclic voltammetry. Then a stem DNA was anchored on the surface of GCE. And with a modifying DNA introduced into the electrode by DNA assembly, a strong ECL signal was obtained. After a DNA modified with ferrocene assembly to the stem DNA, the ECL signal had a sharp decrease due to the quench effect of ferrocene to and the biosensor comes into being a "off" state. With the effect of endonuclease, the ECL signal had a recovery because of the ferrocene being released and the biosensor formed a "on" state. Moreover, the recovery of ECL signal was related to the concentration of endonucleases. Combining specific defined DNA and endonuclease, this method has a potential to detect different endonucleases. In this work, we took the EcoRI as an example to identify the feasibility of ECL biosensor in applying in sensitive detection of endonucleases using a GNPs-graphene signal amplifier. Under optimal condition, the proposed biosensor obtained a low limit of detection (LOD) 5.6×10-5 UmL-1. And the stability, selectivity and reproducibility of the biosensor also were researched. © 2016 Elsevier B.V.


Zhuo B.,Shantou University | Li Y.,Taishan Medicine College | Huang X.,Shantou University | Lin Y.,Shantou University | And 2 more authors.
Sensors and Actuators, B: Chemical | Year: 2015

In this work, we tactfully constructed an electrochemiluminescence (ECL) aptasensing platform with ferrocene-graphene nanosheets (Fc-GNs) using the ferrocene (Fc) as quench unit to tris(2.2′-bipyridyl) ruthenium (II) [Ru(bpy)3 2+] and the Ru(bpy)3 2+ tagged thrombin binding aptamer (Ru-TBA) to recognize the thrombin molecules. Duing to the unique π-π interaction between nucleotides and graphene, the Ru-TBA could be preferentially adsorbed on the surface of ferrocene-graphene nanosheets with the signal generator into off-state. The conformational transformation of Ru-TBA leads to the desorption of Ru-TBA from Fc-GNs after the biosensing electrode incubating with the thrombin solution and the ECL "signal-on" was triggered. With the transformation of luminescence signal from "off" to "on", the biosensor exhibited high sensitivity for the determination of thrombin with a detection limit of 0.21 nM. Particularly, the proposed method could be widely applied to the aptamer-based determination of other target analytes. © 2014 Elsevier Ltd. All rights reserved.


Zhuo B.,Shantou University | Li Y.,Taishan Medicine College | Zhang A.,Shantou University | Lu F.,Shantou University | And 2 more authors.
Journal of Materials Chemistry B | Year: 2014

A solid-state electrochemiluminescence (ECL) biosensor based on a DNA-modified electrode platform that depends on the variation of π-π interaction before and after the binding of target analytes is put forward. The single-stranded DNA (ssDNA) probe was successfully assembled on the surface of a glassy carbon electrode (GCE), which was pre-modified with Ru(bpy) 3 2+ complex and gold nanoparticles (GNPs). The ssDNA probe could strongly adsorb graphene due to the strong π-π interaction between nucleotides and graphene (GN), while in the presence of Hg2+, the conformational transformation of DNA from a single-stranded to a double-stranded structure resulted in inhibited adsorption of GN. With thymine (T)-rich ssDNA as a Hg2+ probe, we prepared the ECL biosensor by using ferrocene-graphene (Fc-GN) as a quenching unit to quench the ECL emission of Ru(bpy)3 2+, and the Hg2+ can be detected by quenching efficiency transformation when the Fc-GN gets away from Ru(bpy) 3 2+. The biosensor exhibited a sensitive response to various ranges of concentration of Hg2+ with a detection limit of 18 pM. The ECL biosensor held great promise in the highly sensitive and selective detection of Hg2+ in natural water. © the Partner Organisations 2014.


Li Y.,Taishan Medicine College | Jia B.,Taishan Medicine College | Wang H.,Taishan Medicine College | Li N.,Shantou University | And 3 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

The interaction of 2-mercaptobenzimidazole (MBI) with human serum albumin (HSA) was studied in vitro by equilibrium dialysis under normal physiological conditions. This study used fluorescence, ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared (FT-IR), circular dichroism (CD) and Raman spectroscopy, atomic force microscopy (AFM) and molecular modeling techniques. Association constants, the number of binding sites and basic thermodynamic parameters were used to investigate the quenching mechanism. Based on the fluorescence resonance energy transfer, the distance between the HSA and MBI was 2.495nm. The ΔG0, ΔH0, and ΔS0 values across temperature indicated that the hydrophobic interaction was the predominant binding Force. The UV, FT-IR, CD and Raman spectra confirmed that the HSA secondary structure was altered in the presence of MBI. In addition, the molecular modeling showed that the MBI-HSA complex was stabilized by hydrophobic forces, which resulted from amino acid residues. The AFM results revealed that the individual HSA molecule dimensions were larger after interaction with MBI. Overall, this study suggested a method for characterizing the weak intermolecular interaction. In addition, this method is potentially useful for elucidating the toxigenicity of MBI when it is combined with the biomolecular function effect, transmembrane transport, toxicological testing and other experiments. © 2012 Elsevier B.V.


PubMed | Taishan Medicine College and Shantou University
Type: Journal Article | Journal: Biosensors & bioelectronics | Year: 2016

Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier. Firstly, the GNPs and graphene were simultaneously deposited on the glassy carbon electrode (GCE) by cyclic voltammetry. Then a stem DNA was anchored on the surface of GCE. And with a modifying DNA introduced into the electrode by DNA assembly, a strong ECL signal was obtained. After a DNA modified with ferrocene assembly to the stem DNA, the ECL signal had a sharp decrease due to the quench effect of ferrocene to and the biosensor comes into being a off state. With the effect of endonuclease, the ECL signal had a recovery because of the ferrocene being released and the biosensor formed a on state. Moreover, the recovery of ECL signal was related to the concentration of endonucleases. Combining specific defined DNA and endonuclease, this method has a potential to detect different endonucleases. In this work, we took the EcoRI as an example to identify the feasibility of ECL biosensor in applying in sensitive detection of endonucleases using a GNPs-graphene signal amplifier. Under optimal condition, the proposed biosensor obtained a low limit of detection (LOD) 5.610


PubMed | Taishan Medicine College and Shantou University
Type: Evaluation Studies | Journal: Analytical and bioanalytical chemistry | Year: 2016

A novel and environmentally friendly reverse fluorescent immunoassay approach was proposed and utilized for sensing human chorionic gonadotropin (HCG) in human serum by coupling a newly prepared and highly fluorescent glutathione-stabilized silver-gold nano-alloy (GSH-AgAuNAs) with magnetic nanoparticles (MNPs). To construct such a reverse system, fluorescent GSH-AgAuNAs and MNPs were first prepared and bio-functionalized with monoclonal antibodies (Mab-I and Mab-II) toward HCG antigen, respectively. Then, the GSH-AgAuNAs functionalized with Mab-I were incubated with HCG, followed by the addition of MNPs attached to Mab-II. Thereafter, a sandwich-type immunoassay could be constructed for determination of HCG owing to the antibody-antigen recognition between the functionalized GSH-AgAuNAs and MNPs. Afterwards, a magnetic collection was employed. Hence, the amount of GSH-AgAuNAs would be reduced through an immuno-magnetic separation, thus weakening the fluorescent intensity. Different from conventional immunoassay, our work determined the quantitative signal by measuring the decreasing gradient fluorescent intensity. Under optimal conditions, the developed reverse method exhibited a wide linear range of 0.5-600ngmL(-1) toward HCG with a detection limit of 0.25ngmL(-1). Additionally, the proposed immunoassay was validated using spiked samples, illustrating a satisfactory result in practical application.

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