Taipei Municipal Jianguo High School

Taipei, Taiwan

Taipei Municipal Jianguo High School

Taipei, Taiwan
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Tsai W.-H.,National Yang Ming University | Tsai W.-H.,Taipei Medical University | Shih C.-H.,Taipei Medical University | Wu H.-Y.,National Yang Ming University | And 7 more authors.
Journal of Cellular Physiology | Year: 2012

Differentiation therapy with all-trans retinoic acid (ATRA) has been used successfully to treat acute promyelocytic leukemia (APL), but such treatment also causes differentiation syndrome (DS) by inducing APL cell infiltration into alveolar spaces. The mechanism underlying the clearance of infiltrated APL cells has not been investigated in detail. Lipoxin A 4 (LXA 4) is an important anti-inflammatory mediator during the resolution of inflammation. In this study, the role of LXA 4 in the cell-cell interaction between alveolar macrophages (AMφ{symbol}; NR8383 cells) and APL NB4 cells was investigated and found that conditioned medium (CM) harvested from ATRA-treated NR8383 (ATRA-NR8383) cells was able to induce the transmigration of ATRA-NB4 cells. However, the pro-migratory activity of CM was attenuated progressively when ATRA-NR8383 cells were co-cultured with increased cell dosages of apoptotic NB4 cells. A significantly higher amount of LXA 4 was released into the CM by ATRA-NR8383 cells when they were co-cultured with apoptotic ATRA-NB4 cells. Expression of a receptor for LXA 4 (ALX/FPR2) was enhanced in both ATRA-NB4 cells and ATRA-NR8383 cells. Exogenous LXA 4 treatment was able to inhibit the transmigration of ATRA-NB4 cells and induce the phagocytic clearance of apoptotic cells by ATRA-NR8383 cells. The anti-migratory activity of exogenous LXA 4 was attenuated by pre-treating ATRA-NB4 cells with an ALX/FPR2 inhibitor. We conclude that AMφ{symbol}-derived LXA 4 plays an important role in the interaction between AMφ{symbol} and APL cells and that this contributes to clearance of apoptotic APL cells. © 2011 Wiley Periodicals, Inc.


Tsai W.-H.,National Yang Ming University | Tsai W.-H.,Taipei Medical University | Chien H.-Y.,National Yang Ming University | Shih C.-H.,Taipei Medical University | And 6 more authors.
Journal of Cellular Physiology | Year: 2012

Annexin A1 (AnxA1) originating from mature neutrophils and their microparticles (MPs) plays an important anti-inflammatory role during the resolution phase of inflammation. However, the role of AnxA1 during the process of granulocytic differentiation is still unknown. All-trans retinoic acid (ATRA) can induce acute promyelocytic leukemic (APL) cells to differentiate along the granulocytic lineage and has been used successfully in treating APL patients. In this study, we investigated whether or not AnxA1 contributed to the anti-inflammatory properties of ATRA-treated APL (NB4; ATRA-NB) cells using the transmigratory and adhesive assays. We found that ATRA was able to enhance the surface expression of AnxA1 and its receptor (FPR2/ALX) and the release of AnxA1-containing MPs from ATRA-NB4 cells, while the expression of annexin V was not elevated on the latter cells. Further studies demonstrated that exogenous AnxA1 could inhibit ATRA-NB4 cells in their transmigratory activity and adhesion to endothelial cells. In addition, the transmigratory activity of ATRA-NB4 cells can be significantly enhanced by pretreatment with a FPR2/ALX neutralizing antibody, suggesting that endogenous AnxA1 may contribute to the anti-migratory effects. Finally, ATRA-NB4-derived MPs could also inhibit recipient cells in their transmigratory and adhesive activities and these anti-inflammatory effects could be inhibited by pretreatment of MPs with a specific anti-AnxA1 antibody. Flowcytometry studies further demonstrated that FITC-labeled AnxA1 could be transported from MPs to the membrane of recipient ATRA-NB4 cells. We conclude that biologically active AnxA1 may play a role in the anti-inflammatory properties of ATRA-treated APL cells during the process of granulocytic differentiation. © 2012 Wiley Periodicals, Inc.


Yen M.-H.,National Taiwan Ocean University | Yen H.-K.,Taipei Municipal Jianguo High School | Yu C.,National Ilan University
IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems | Year: 2015

Fan proposed a set of hyperuniversal switch blocks (HUSBs), called Fan's (4, , w)-HUSBs in this paper. He also proved that his design with w less than 6 was optimum. That is, they used the least switches to achieve hyperuniversal routability. However, we find a counter example to show that there is an unnecessary switch in Fan's (4, 4)-HUSB. Namely, we propose a new (4, 4)-HUSB, water-molecule-shaped switch block (WMSB), proving (4, 4)-WMSB with 24 switches is optimal. Furthermore, we also propose optimal (4, 7)-WMSB, which is a (4, 7)-HUSB with 42 switches. © 1982-2012 IEEE.


Chen C.-Y.,Institute of Molecular and Cellular Biology | Ho A.,National Taiwan University | Huang H.-Y.,Taipei Municipal Jianguo High School | Juan H.-F.,Institute of Molecular and Cellular Biology | And 2 more authors.
Scientific Reports | Year: 2014

The protein-protein interaction (PPI) network offers a conceptual framework for better understanding the functional organization of the proteome. However, the intricacy of network complexity complicates comprehensive analysis. Here, we adopted a phylogenic grouping method combined with force-directed graph simulation to decompose the human PPI network in a multi-dimensional manner. This network model enabled us to associate the network topological properties with evolutionary and biological implications. First, we found that ancient proteins occupy the core of the network, whereas young proteins tend to reside on the periphery. Second, the presence of age homophily suggests a possible selection pressure may have acted on the duplication and divergence process during the PPI network evolution. Lastly, functional analysis revealed that each age group possesses high specificity of enriched biological processes and pathway engagements, which could correspond to their evolutionary roles in eukaryotic cells. More interestingly, the network landscape closely coincides with the subcellular localization of proteins. Together, these findings suggest the potential of using conceptual frameworks to mimic the true functional organization in a living cell.


Lee H.-C.,National Taiwan University | Chen Y.-J.,National Taiwan University | Liu Y.-W.,National Taiwan University | Lin K.-Y.,National Taiwan University | And 6 more authors.
Nucleic Acids Research | Year: 2011

Upstream open reading frame (uORF)-mediated translational inhibition is important in controlling key regulatory genes expression. However, understanding the underlying molecular mechanism of such uORF-mediated control system in vivo is challenging in the absence of an animal model. Therefore, we generated a zebrafish transgenic line, termed huORFZ, harboring a construct in which the uORF sequence from human CCAAT/enhancer-binding protein homologous protein gene (huORF chop) is added to the leader of GFP and is driven by a cytomegalovirus promoter. The translation of transgenic huORF chop-gfp mRNA was absolutely inhibited by the huORF chop cassette in huORFZ embryos during normal conditions, but the downstream GFP was only apparent when the huORFZ embryos were treated with endoplasmic reticulum (ER) stresses. Interestingly, the number and location of GFP-responsive embryonic cells were dependent on the developmental stage and type of ER stresses encountered. These results indicate that the translation of the huORF chop-tag downstream reporter gene is controlled in the huORFZ line. Moreover, using cell sorting and microarray analysis of huORFZ embryos, we identified such putative factors as Nrg/ErbB, PI3K and hsp90, which are involved in huORF chop-mediated translational control under heat-shock stress. Therefore, using the huORFZ embryos allows us to study the regulatory network involved in human uORF chop-mediated translational inhibition. © 2011 The Author(s).


Tan W.-H.,National Taiwan University | Liu T.-H.,Taipei Municipal Jianguo High School | Lin Y.K.,National Taiwan University | Hsu Y.-F.,National Taiwan Normal University
Zoology | Year: 2014

Anthropogenic introduction of a plant species may cause novel encounters between the plant and local herbivores, and initiate evolutionary changes in host plant usage by herbivores. Until recently the endemic aquatic plant Hygrophila pogonocalyx was endangered and had a restricted distribution in Taiwan. Massive restoration efforts since 1997 have led to an expansion of the plant's distribution and a novel encounter between it and an Asian butterfly, the chocolate pansy, Junonia iphita (Nymphalidae). This butterfly appears to have colonized H. pogonocalyx, switching from its original host, Strobilanthes penstemonoides var. formosana. In the present study, we aimed to investigate whether the utilization of H. pogonocalyx as a host plant has initiated a differentiation between butterflies using the novel and the original hosts. To this purpose we collected butterflies from patches of the two host plants which grow sympatrically. We tested oviposition preference for the two hosts and larval performance on them. Female adults exhibited distinct oviposition preference toward the host plant their mothers preferred. Offspring showed greater survivorship and pupal weight when fed on the host plant their mothers preferred. Male adults displayed territorial behaviors on the host plant that their mothers had preferred. Finally, the survival rate of offspring produced from cross-mating between individuals with different host plant preference was lower than that of non-hybrids. Taken together, we suggest that genetic differentiation has occurred between individuals preferring H. pogonocalyx versus S. penstemonoides as host plants via host shifting. This process was likely induced by the mass restoration of the formerly rare and endangered plant species. © 2014 Elsevier GmbH.


PubMed | Taipei Municipal Jianguo High School, National Taiwan University, National Yang Ming University and Institute of Molecular and Cellular Biology
Type: | Journal: Scientific reports | Year: 2014

The protein-protein interaction (PPI) network offers a conceptual framework for better understanding the functional organization of the proteome. However, the intricacy of network complexity complicates comprehensive analysis. Here, we adopted a phylogenic grouping method combined with force-directed graph simulation to decompose the human PPI network in a multi-dimensional manner. This network model enabled us to associate the network topological properties with evolutionary and biological implications. First, we found that ancient proteins occupy the core of the network, whereas young proteins tend to reside on the periphery. Second, the presence of age homophily suggests a possible selection pressure may have acted on the duplication and divergence process during the PPI network evolution. Lastly, functional analysis revealed that each age group possesses high specificity of enriched biological processes and pathway engagements, which could correspond to their evolutionary roles in eukaryotic cells. More interestingly, the network landscape closely coincides with the subcellular localization of proteins. Together, these findings suggest the potential of using conceptual frameworks to mimic the true functional organization in a living cell.


Young C.W.,Section of Invertebrate Zoology | Chu C.L.,Taipei Municipal Jianguo High School
Annals of Carnegie Museum | Year: 2010

The external anatomy of the last instar larva and pupa of the crane fly, Indotipula demarcata (Brunetti, 1911) (Diptera: Tipulidae: Tipulinae), is described and illustrated from specimens collected in Taiwan. Comments concerning natural history and microhabitats of larvae are provided. This is the first detailed description with illustrations for the larva and pupa of Indotipula Edwards, 1931, Pupal characters resemble those of most species from advanced lineages within the subfamily Tipulinae.


Wang T.-C.,Taipei Municipal Jianguo High School
Proceedings - 2015 International Conference on Learning and Teaching in Computing and Engineering, LaTiCE 2015 | Year: 2015

The concept of binary, which is the basis of how computer works, is often taught in Taiwan's high school computer courses as the introductory unit of the class 'Introduction to Computers'. In the past, teachers used to lecture through binary concepts such as the decimal binary conversion, ASCII code transformation, and several traditional courses. However, most students did not know why they needed to learn binary concepts, and seemed less interested in learning. To enhance the effectiveness of learning, we incorporated some examples corresponding to each binary concept to link students' life experiences to the concepts in order to make learning meaningful. In this paper, we will share our teaching materials for the binary concept from the course 'Introduction to Computers'. The lesson starts from how computer stores values, text or image data to introduce basic concepts of binary data interpretation and hex conversion. Afterwards, a combination of simple logic gates adder instance is introduced to guide students to learn how to perform a binary compute operation. Finally, software CPU simulator is covered to allow students to learn and visualize how computer uses binary data and basic operations to perform some specific compute functions. © 2015 IEEE.


Lai C.-I.,Taipei Municipal Jianguo High School | Chen W.-T.,Taipei Municipal Jianguo High School | Lai Y.-T.,National Yang Ming University | Lo C.-M.,National Yang Ming University
IFMBE Proceedings | Year: 2015

We investigated the effect of transforming growth factor-beta1 (TGF-β1) on cell micromotion and wound healing migration of breast adenocarcinoma cells, MDA-MB- 231. Central to this work was the use of electric cell-substrate impedance sensing (ECIS), a cell-based biosensor that monitors motility and other morphology changes of cells adherent on small gold electrodes. To detect the alternation of cell micromotion in response to 10 ng/ml of TGF-β1 challenge, timeseries impedance fluctuations of cell-covered electrodes were monitored and the values of variance, power slope, and Hurst coefficient were calculated to verify the difference. These measures describe the motile and persistent behavior of these cells in culture. Furthermore, ECIS wounding assay was used to wound and monitor the healing process of TGF-β1 treated cell layers. Our data indicated that TGF-β1 treated MDA-MB- 231 cells, in comparison to control cells, demonstrated higher fluctuation amplitude and unreduced long-term correlation for about 20 hours long. In addition, the wound healing rate of MDA-MB-231 cells significantly increased after cells were treated with 10 ng/ml of TGF-β1 though the effect decreased along with time. The results suggest that treatment of MDAMB- 231 cells with TGF-β1 facilitates their scattering behavior. © Springer International Publishing Switzerland 2015.

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