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Tsai H.-T.,Taipei Medical University | Tsai H.-T.,Chung Shan Medical University | Hsieh M.-J.,Changhua Christian Hospital | Hsieh M.-J.,Chung Shan Medical University | And 9 more authors.
Tumor Biology | Year: 2014

The purpose of this study was to investigate genetic impact of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) gene polymorphisms on the susceptibility and clinicopathological characteristics of hepatocellular carcinoma (HCC). A total of 759 subjects, including 530 healthy controls and 229 patients with hepatocellular carcinoma, were recruited in this study. Allelic discrimination of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) polymorphisms was assessed with the ABI StepOne™ Real-Time PCR System. Among women group, individuals with TC or CC alleles of TIMP-3 -1296 T>C gene polymorphism protected against HCC (AOR = 0.35, 95% confidence interval (CI) = 0.12–0.97; p = 0.04) compared to individuals with TT alleles, after adjusting for other confounders. Also, women with TC alleles and with TC or CC alleles of TIMP-4 -55 T>C polymorphisms had a 2.52-fold risk (95%CI = 1.23–5.13; p = 0.01) and 2.47-fold risk (95%CI = 1.26–4.87; p = 0.008) of developing HCC compared to individuals with TT alleles, after adjusting for other confounders. There was no synergistic effect between gene polymorphism and environmental risk factors, including tobacco and alcohol consumptions and clinical statuses of HCC as well as serum expression of liver-related clinicopathological markers. In conclusion, gene polymorphisms of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) play a role in the susceptibility of HCC among Taiwan women. © 2014, International Society of Oncology and BioMarkers (ISOBM). Source

Fan Y.-G.,Taichung Armed Force General Hospital | Hu C.-W.,National Cheng Kung University | Chu C.,National Chiayi University | Chiu K.-C.,National Chiayi University | Weng B.B.C.,National Chiayi University
Food Research International | Year: 2012

Heavily evidenced in antimicrobial effects of microbial β-(1,3)(1,6)-glucan, the botanical β-(1,3)(1,4)-glucan is mostly shown to effectively control blood cholesterol level. S. Typhimurium belongs to food-borne zoonoses often causing worldwide epidemic outbreaks in animals and human with severe diarrhea and gastrointestinalitis. Cereal soups are commonly prescribed as neutraceutical for rehydration purpose and a sustaining therapy. Botanical β-(1,3)(1,4)-glucan is easily released in boiled cereal soup β-(1,3)(1,4)-Glucans on modulating host defense to enteric infectious agents is seldom reported. Our results demonstrated that barley β-(1,3)(1,4)-glucans effectively increased the murine macrophage cell line RAW264.7 against S. Typhimurium infection through antibacterial lysozyme activity (P < 0.001), not the production of intracellular reactive oxygen species. Furthermore, barley β-(1,3)(1,4)-glucans upregulated the gene expressions of its receptor dectin-1. In conclusion, barley β-(1,3)(1,4)-glucan induces a mild immune response with increasing antibacterial lysozymes through up-regulating its receptors dectin-1 and lysozyme M gene expressions. © 2011 Elsevier Ltd. Source

Chen T.-S.,China Medical University at Taichung | Chang M.-H.,Taichung Armed Force General Hospital | Kuo W.-W.,China Medical University at Taichung | Lin Y.-M.,Changhua Christian Hospital | And 7 more authors.
Pharmaceutical Biology | Year: 2013

Context: Statistical and clinical reports indicate that betel nut chewing is strongly associated with progression of oral cancer because some ingredients in betel nuts are potential cancer promoters, especially arecoline. Early diagnosis for cancer biomarkers is the best strategy for prevention of cancer progression. Several methods are suggested for investigating cancer biomarkers. Among these methods, gel-based proteomics approach is the most powerful and recommended tool for investigating biomarkers due to its high-throughput. However, this proteomics approach is not suitable for screening biomarkers with molecular weight under 10 KDa because of the characteristics of gel electrophoresis. Objective: This study investigated biomarkers with molecular weight under 10 KDa in rats with arecoline challenge. Materials and methods: The centrifuging vials with membrane (10 KDa molecular weight cut-off) played a crucial role in this study. After centrifuging, the filtrate (containing compounds with molecular weight under 10 KDa) was collected and spotted on a sample plate for MALDI-TOF mass spectrometry analysis. Results: Compared to control, three extra peaks (m/z values were 1553.1611, 1668.2097 and 1740.1832, respectively) were found in sera and two extra peaks were found in heart tissue samples (408.9719 and 524.9961, respectively). These small compounds should play important roles and may be potential biomarker candidates in rats with arecoline. Discussion and conclusions: This study successfully reports a mass-based method for investigating biomarker candidates with small molecular weight in different types of sample (including serum and tissue). In addition, this reported method is more time-efficient (1 working day) than gel-based proteomics approach (5~7 working days). © 2013 Informa Healthcare USA, Inc. Source

Pien H.-C.,Taichung Armed Force General Hospital | Huang H.-I.,Central Taiwan University of Science and Technology | Tu Y.-Y.,Taichung Armed Force General Hospital | Kuo W.-H.,Taichung Armed Force General Hospital | Chen L.-M.,Taichung Armed Force General Hospital
Biomarkers and Genomic Medicine | Year: 2013

Atherosclerosis is a chronic inflammatory vascular disease, and an association between elevated blood lipids and atherosclerosis has previously been recognized. Low-density lipoprotein cholesterol (LDLc) levels have also been recognized as indicators of cardiovascular complications, but two people who have exactly the same LDLc concentrations may have different risk factors, and the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) guidelines recommend patching non-high-density lipoprotein cholesterol (non-HDLc) as another indicator. The present study investigated diabetic dyslipidemia to determine if there is a correlation between dyslipidemia and C-reactive protein (CRP). The data shows that non-HDLc was an independent risk factor for cardiovascular events in patients with diabetes and that CRP could indicate the risk of future cardiovascular events in both high-risk and healthy individuals. © 2013. Source

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