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Yang F.B.,Taian City Central Hospital
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To study the effects of curcumin on vascular endothelial injuries induced by radiation and the mechanism. Human umbilical vein endothelial cells (HUVEC) were isolated, cultured and divided into the control group and 4 groups exposed to 3-ray at the doses of 2, 4, 6 and 8 Gy. Cellular morphological and ultrastructural changes were examined under light microscopy and electron microscopy respectively. Flow cytometry was used to detect the cellular apoptosis, necrosis and intracellular reactive oxygen species(ROS) generation. The contents of Lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the cultures were measured before and after irradiation. The results of cellular morphological and ultrastructural ex-aminations shown that the typical apoptotic changes appeared after irradiation. The rates of apoptosis and necrosis in groups pretreated with curcumin were significantly lower than those in other groups (P<0.05). LDH and MDA in the irradiation groups were significantly higher than those in curcumin pretreatment groups (P<0.05). The ROS generation in radiation groups significantly increased with the radiation doses, as compared with the groups pre-treated with curcumin (P<0.05). Irradiation induced the apoptosis and necrosis of HUVEC, and increased significantly the intracellular LDH and MDA levels in a dose-dependent. Curcumin had the protective effects on HUVEC from the apoptosis and necrosis induced by radiation. Source


Qiao J.,Zhejiang University | Gao P.,Taian City Central Hospital | Jiang X.,Zhejiang University | Fang H.,Zhejiang University
Annals of Clinical Microbiology and Antimicrobials | Year: 2013

Background: Protein farnesylation is an important tosttranslational modification in fungi. We evaluated the antifungal activity of two farnesyltransferase inhibitors against clinical isolates of Aspergillus and Candida.Methods: Disk diffusion assay and broth microdilution assay were used to determine the antifungal susceptibility of two farnesyltransferase inhibitors (manumycin A and tipifarnib) against clinical isolates of Aspergillus and Candida.Results: Disk diffusion assay demonstrated both agents had activity against Aspergillus and Candida. The minimal inhibitory concentration (MIC) ranges for manumycin A against Aspergillus and Candida were 200 to 400 μM and 13 to >25 μM, respectively. Unfortunately, the MIC were vastly higher than the concentrations that inhibit the proliferation and viability of mammalian cells. The MICs of tipifarnib against Aspergillus and Candida were >1600 μM.Conclusion: The outcome of present study showed that farnesyltransferase inhibitors have activity against Aspergillus and Candida. This suggests that farnesyltransferase may be used as anifungal target in designing and developing new drugs. © 2013 Qiao et al.; licensee BioMed Central Ltd. Source


Qiao J.,Zhejiang University | Zhou G.,Taian City Central Hospital | Ding Y.,Zhejiang University | Zhu D.,Zhejiang University | Fang H.,Zhejiang University
Journal of the Neurological Sciences | Year: 2011

Thymomas are associated with paraneoplastic autoimmune diseases at a high frequency. It is rare that four paraneoplastic autoimmune disorders co-occur in a single patient. We describe a thymoma patient with diagnoses of myasthenia gravis, vitiligo, alopecia areata, and oral lichen planus associated with a thymoma. After thymectomy, the weakness, vitiligo, alopecia and mucocutaneous lesions were improving progressively, possibly implicating the thymoma in initiating these autoimmune conditions. We believe that this is the first report of this particular combination of multiple paraneoplastic syndromes associated with thymoma. © 2011 Elsevier B.V. All rights reserved. Source


Xue F.,Taian City Central Hospital | Yi H.,Jinan Maternity and Child Care Hospital | Ru X.,Hebei Medical University
International Journal of Clinical and Experimental Pathology | Year: 2016

Cervical cancer is a disease of high incidence, affecting numerous female patients' health and life. miR-21 is reported to play oncogenic roles in various cancers including cervical cancer, but its correlation with cervical cancer pathological progression is not fully understood. This study aims at revealing the expression pattern of miR-21 in the tissues of cervical intraepithelial neoplasia (CIN) and cervical cancer patients of different pathological grades. miR-21 levels in 35 CIN and 35 cervical cancer specimens were detected by qRT-PCR and compared to 40 normal specimens. Then in the 35 cervical cancer specimens, miR-21 levels were compared in different cancer variables. Its roles in cervical cancer cell migration and invasion were again verified by Transwell experiments after vector transfection to overexpress pre-miR-21 or miR-21 sponge in HeLa cells. miR-21 was promoted in CIN tissues compared to normal tissues (P < 0.05), and elevated in cervical cancer tissues compared to CIN tissues (P < 0.05). In cervical cancer tissues, miR-21 expression did not vary significantly with different patient ages or tumor sizes (P > 0.05), but it was significantly up-regulated in cancer tissues with higher pathology grades, clinical stages and lymphatic or distant metastasis (P < 0.05), which was supported by Transwell results that miR-21 promoted HeLa cell migration and invasion. These results indicated that the up-regulation of miR-21 might be associated with severer cervical cancer grades, implying the role of miR-21 as a possible indicator for the pathological progression of cervical cancer. Source


Wu J.,Fudan University | Song T.,Shandong University | Liu S.,Taian City Central Hospital | Li X.,Shandong University | And 2 more authors.
Molecular Medicine Reports | Year: 2015

Icariside II (IS) is a metabolite of icariin, which is derived from Herba Epimedii. In the present study, the antiproliferative effects of IS on A375 human melanoma cells were examined in vitro and a possible mechanism through the ROS-p38-p53 pathway is discussed. A cell WST.8 assay revealed that treatment with IS markedly reduced cell viability from 77 to 21% (25 and 100 μM, respectively), and cell counting demonstrated that IS treatment reduced cell proliferation. IS treatment also induced cell cycle arrest of A375 cells at the G0/G1 and G2/M transitions and inhibited the expression of cell-cycle related proteins, including cyclin E, cyclin.dependent kinase 2 (CDK2), cyclin B1 and phosphorylated cyclin-dependent kinase 1 (P.CDK1). In this study, it was determined that IS inhibits cell proliferation and induces cell cycle arrest through the generation of reactive oxygen species and activation of p38 and p53-These findings were further supported by the evidence that pretreatment with N-acetyl-L-cysteine, SB203580 or pifithrin-α significantly blocked IS-induced reduction of cell viability, increase of cell death and cell cycle arrest. In conclusion, IS inhibits cell proliferation and induces cell cycle arrest. Crucially, it was confirmed that these effects were mediated at least in part by activating the ROS-p38-p53 pathway. Source

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