Taian Academy Of Agricultural Science

Tai’an, China

Taian Academy Of Agricultural Science

Tai’an, China
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Zhang Z.-G.,Shandong Agricultural University | Lv G.-D.,Shandong Agricultural University | Lv G.-D.,Taian Academy of Agricultural Science | Li B.,Shandong Agricultural University | And 5 more authors.
PLoS ONE | Year: 2017

Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPSwas developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower. © 2017 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


HE F.,Shandong Agricultural University | BAO Y.,Shandong Agricultural University | QI X.,Taian Academy of Agricultural science | MA Y.,Shandong Agricultural University | And 2 more authors.
Journal of Genetics | Year: 2017

Thinopyrum ponticum (2 n=70) serves as a valuable gene pool for wheat improvement. Line SN0224, derived from crosses between Th. ponticum and the common wheat cultivar Yannong15, was identified in the present study. Cytogenetic observations showed that SN0224 contains 42 chromosomes in the root-tip cells and 21 bivalents in the pollen mother cells, thereby demonstrating its cytogenetic stability. Genomic in situ hybridization, probed with the total genomic DNA of Th. ponticum, produced hybridization signals in the distal region of two wheat chromosome arms. After inoculation with the Blumeria graminis f. sp. tritici (Bgt) isolates, SN0224 exhibited immunity. Segregation in F 1s and F 2s from the cross SN0224/cv. Huixianhong indicated that SN0224 carries a single dominant gene for powdery mildew (Pm) resistance, which was temporarily designated PmSn0224. Three markers Barc212, Xwmc522 and Xbarc1138 were detected to be linked with PmSn0224. Based on the locations of the markers, PmSn0224 was located on the chromosome 2A. None of the three markers above is linked with the previously reported PM resistance genes on chromosome 2A, and none of the previously reported PM resistance genes on chromosome 2A is related to Th. ponticum. Therefore, PmSn0224 is likely a novel gene putatively from Th. ponticum. © 2017 Indian Academy of Sciences


Ding H.,Shandong Peanut Research Institute | Zhang Z.,Shandong Peanut Research Institute | Kang T.,Taian Academy of Agricultural science | Dai L.,Shandong Peanut Research Institute | And 3 more authors.
International Journal of Plant Production | Year: 2017

The effects of two water regimes (well-watered condition or drought stress) on root length, root surface area and root volume were tested on two peanut genotypes (the drought-resistant variety HuaYu 22 (HY22) and the drought-sensitive variety HuaYu 23 (HY23)), measured 101 days after sowing. The roots were sampled from the upper (0–40 cm) and deeper (40–100 cm) soil layers. Root diameter was measured to the nearest 0.5 mm in describing its distribution. Total dry weight and pod yield were measured at harvest. The drought tolerance index of pod yield and the harvest index in HY22 were higher than those in HY23. The total root length density (RLD), total root surface area and volume were significantly higher for HY22 than HY23. The RLD in the deeper soil layer was lower for HY23 than HY22. Under drought stress, the percent RLD in the deeper layers increased in both genotypes. Compared to well-watered condition, the total root surface area and root volume in the upper soil layer were lower under drought stress and root traits in deeper soil layers were higher. Drought stress had no impact on very fine roots (diameter < 0.5 mm) of HY22 in the deeper soil layer but lowered their share in HY23 markedly. The RLD and root surface area in the deeper soil layer were related to the pod yield of peanut. This finding could be useful in growing peanut under drought conditions. © 2017, GORGAN UNIV AGRICULTURAL SCIENCES AND NATURAL RESOURCES. All rights reserved.


Wang W.,Central China Normal University | Wang W.,Xi'an Modern Chemistry Research Institute | Zhang S.-S.,Central China Normal University | Zhou Y.,Central China Normal University | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2016

On the basis of our work on the modification of alkylphosphonates 1, a series of α-(substituted phenoxybutyryloxy or valeryloxy)alkylphosphonates (4-5) and 2-(substituted phenoxybutyryloxy)alkyl-5,5-dimethyl-1,3,2-dioxaphosphinan-2-one (6) were designed and synthesized. The bioassay results indicated that 14 of title compounds 4 exhibited significant postemergence herbicidal activity against velvetleaf, common amaranth, and false daisy at 150 g ai/ha. Compounds 5 were inactive against all tested weeds. Compounds 6 exhibited moderate to good inhibitory effect against the tested dicotyledonous weeds. Structure-activity relationship (SAR) analyses showed that the length of the carbon chain as linking bridge had a great effect on the herbicidal activity. Broad-spectrum tests of compounds 4-1, 4-2, 4-9, 4-30, and 4-36 were carried out at 75 g ai/ha. Especially, 4-1 exhibited 100% inhibition activity against the tested dicotyledonous weeds, which was higher than that of glyphosate. © 2016 American Chemical Society.


PubMed | Taian Academy of Agricultural science and Central China Normal University
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2016

On the basis of our work on the modification of alkylphosphonates 1, a series of -(substituted phenoxybutyryloxy or valeryloxy)alkylphosphonates (4-5) and 2-(substituted phenoxybutyryloxy)alkyl-5,5-dimethyl-1,3,2-dioxaphosphinan-2-one (6) were designed and synthesized. The bioassay results indicated that 14 of title compounds 4 exhibited significant postemergence herbicidal activity against velvetleaf, common amaranth, and false daisy at 150 g ai/ha. Compounds 5 were inactive against all tested weeds. Compounds 6 exhibited moderate to good inhibitory effect against the tested dicotyledonous weeds. Structure-activity relationship (SAR) analyses showed that the length of the carbon chain as linking bridge had a great effect on the herbicidal activity. Broad-spectrum tests of compounds 4-1, 4-2, 4-9, 4-30, and 4-36 were carried out at 75 g ai/ha. Especially, 4-1 exhibited 100% inhibition activity against the tested dicotyledonous weeds, which was higher than that of glyphosate.


Guo Y.,Shandong Agricultural University | Guo Y.,Taian Academy of Agricultural science | Sun J.,Shandong Agricultural University | Zhang G.,Shandong Agricultural University | And 5 more authors.
Field Crops Research | Year: 2013

Glutamine synthetase (GS) is a key enzyme in the formation of the amino acid glutamine during N assimilation. The characterization of GS genes and the development of functional markers are important for marker-assisted selection (MAS) in wheat breeding programs. In the present study, the full-length genomic DNA (gDNA) sequence of TaGS1a was obtained from 60 wheat varieties. TaGS1a comprises 3415. bp and has eleven exons and ten introns. Nine single nucleotide polymorphisms (SNPs) and two insertions and deletions of DNA segments (InDels) were detected in introns, resulting two haplotypes: Hap 1 and Hap 2. A cleaved amplified polymorphic sequence (CAPS) marker was developed to distinguish the two haplotypes. The TaGS1a-CAPS marker was located on chromosome 6D using Chinese Spring nullisomic-tetrasomic lines, and mapped at 2.5 cM from the SSR marker barc1121b in a RIL population. The completely corresponding results between quantitative trait locus (QTL) analysis and association analysis suggested that the TaGS1a gene had functions for grain size traits, including thousand grain weight (TGW), grain width (GW), grain height (GH), GL/GW ratio (GLW), factor form density (FFD), grain area (GA) and grain volume (GV) during maturity stage, and for the ratio of root/shoot for dry weight and nitrogen, phosphorus, potassium contents (RSDW, RSNC, RSPC and RSKC) during seedling stage. © 2013 Elsevier B.V.


Sun Y.,Shandong Agricultural University | Liu Z.,Taian Academy of Agricultural science | Wang J.,Shandong Agricultural University | Yang S.,Shandong Agricultural University | And 2 more authors.
Ultrasonics Sonochemistry | Year: 2013

In this study, an aqueous ionic liquid based ultrasonic assisted extraction (ILUAE) method for the extraction of the four acetophenones, namely 4-hydroxyacetophenone (1), 2,5-dihydroxyacetophenone (2), baishouwubenzophenone (3) and 2,4-dihydroxyacetophenone (4) from the Chinese medicinal plant Cynanchum bungei was developed. Three kinds of aqueous l-alkyl-3-methylimidazolium ionic liquids with different anion and alkyl chain were investigated. The results indicated that ionic liquids (ILs) showed remarkable effects on the extraction efficiency of acetophenones. In addition, the ILUAE, including several ultrasonic parameters, such as the ILs concentration, solvent to solid ratio, power, particle size, temperature, and extraction time have been optimized. Under these optimal conditions (e.g., with 0.6 M [C4MIM]BF 4, solvent to solid ratio of 35:1, power of 175 W, particle size of 60-80 mesh, temperature of 25 °C and time of 50 min), this approach gained the highest extraction yields of four acetophenones 286.15, 21.65, 632.58 and 205.38 μg/g, respectively. The proposed approach has been evaluated by comparison with the conventional heat-reflux extraction (HRE) and regular UAE. The results indicated that ILUAE is an alternative method for extracting acetophenones from C. bungei. © 2012 Elsevier B.V. All rights reserved.


Zhang Z.,Shandong Agricultural University | Zhou C.,Taian Academy of Agricultural science | Xu Y.,Shandong Agricultural University | Huang X.,Shandong Agricultural University | And 2 more authors.
Journal of Pest Science | Year: 2016

Tea plants (Camellia sinensis (L.) O. Kuntze) are often colonized by economically important insect pests such as tea green leafhoppers (Empoasca onukii Matsuda) and green plant bugs (Apolygus lucorum Meyer-Dür) in northern China. Aromatic plant volatiles can exert repellent effects on insect herbivores. To evaluate the impact of intercropping on the populations of two tea pests and natural enemies in tea plantations, field experiments were conducted in which four aromatic plants (Cassia tora, Medicago sativa, Leonurus artemisia, and Mentha haplocalyx) were intercropped with tea plants and compared with tea monoculture in 2014 and 2015. The counts of arthropods conducted on tea plants showed that intercropping C. tora and L. artemisia with tea plants significantly reduced the E. onukii population levels in the tea plantation. The abundance of A. lucorum was variable during the study and not significantly lower in various intercropping plots than monocropping plots. Y-tube olfactometer assays showed that volatile organic compounds derived from C. tora and M. sativa significantly affected the behavior of E. onukii and that M. haplocalyx odors can be used to mask host plant odors and are repellent to A. lucorum. Among natural enemies, coccinellids occurred often on tea plants during growing seasons. Intercropping tea plants with C. tora also promoted a marked increase in the natural enemies of tea pests, including coccinellids, lacewings, spiders, and parasitoids. Our findings suggest that the sustainable pest management achieved by intercropping offers an alternative strategy for the control of important tea pests. © 2016 Springer-Verlag Berlin Heidelberg


Liu Z.,Taian Academy of Agricultural science | Wang J.,Shandong Agricultural University | Bi J.,Taian Academy of Agricultural science | Bi Y.,Taian Academy of Agricultural science | And 3 more authors.
Separation Science and Technology | Year: 2011

Supercritical fluid extraction (SFE) was used to extract three isoflavonoids including irigenin, irisfloretin and dichtomitin from Belamcanda chinensis (L.) DC. The parameters including pressure, temperature, sample particle size, and flow rate of CO 2 were optimized with an orthogonal test. Under the optimized conditions of 15 MPa, 55°C, a sample particle size of 20-40 mesh and CO 2 flow rate of 40 L h -1. The process was then scaled up by 10 times using a preparative SFE system. The yield of the crude extract from SFE was 4.1%, which contained irigenin, irisfloretin, and dichtomitin 0.71%, 0.49%, and 0.05%, respectively. To compare the extraction methods, Soxhlet Extraction (SE) was performed. The results indicated that SFE was better than SE. Irigenin, irisfloretin, and dichtomitin in the SFE extract were then separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (2:4:3:3, v/v). From 5.0 g of dry crude extract, 27.8 mg irigenin, 16.4 mg irisfloretin, and 2.1 mg dichtomitin were obtained at purities of 97.1%, 96.4%, and 98.0%, respectively, as determined by HPLC-PDA. These results well indicate that SFE and HSCCC are very powerful techniques for the extraction and purification of irigenin, irisfloretin, and dichtomitin from B. chinensis. © 2011 Taylor and Francis Group, LLC.


Sun Y.,Shandong Agricultural University | Liu Z.,Taian Academy of Agricultural science | Wang J.,Shandong Agricultural University
Separation and Purification Technology | Year: 2011

This study investigated the use of ultrasound-assisted extraction (UAE) to improve the extraction efficiency of the classical solvent extraction techniques such as maceration extraction (ME) and soxhlet extraction (SE) to extract five isoflavones (tectoridin, iristectorin B, iristectorin A, tectorigenin and iristectorigenin A) from Iris tectorum. The effects of various factors such as extraction solvent, solvent concentration, temperature, solvent to solid ratio, ultrasound power, extraction time and particle size on the yield of target components were investigated. The optimal UAE conditions found were: 70% (v/v) methanol solution, temperature 45 °C, solvent to solid ratio 15 ml/g, ultrasound power 150 W, extraction time 45 min and particle size 60-80 mesh. The results indicated that compared with ME at 18 h and SE at 6 h, UAE gave the highest extraction yields of tectoridin, iristectorin B, iristectorin A, tectorigenin, iristectorigenin A and total isoflavones at 45 min. The results indicated that UAE was an alternative method for extracting isoflavones from I. tectorum. © 2011 Elsevier B.V. All rights reserved.

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