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Emprm V.,ESI Post Graduate Institute of Medical science and Research PGIMSR | Rajanandh M.G.,Sri Ramachandra University | Nageswari A.D.,Tagore Medical College and Hospital
Journal of Clinical and Diagnostic Research | Year: 2016

Introduction: Chronic airway inflammation and remodelling are fundamental features of asthma. The molecular phenotypes in asthma are Th2 high and Th2 low. Serum periostin is a biomarker which aid in understanding Th2 high eosinophilic asthma. Aim: The present study aimed to identify whether or not serum periostin is a systemic biomarker for eosinophilic airway inflammation in asthmatics. Materials and Methods: The study was designed as a prospective, case control study. Patients who presented with consistent symptoms of asthma and confirmed by spirometry with reversibility were the cases. The controls were healthy subjects who had no history of lung disease with normal lung function. The sputum and blood samples were collected from both the groups. Sputum eosinophils, Absolute Eosinophil Counts (AEC) and serum periostin levels were compared between the groups. Results: The study comprised of 101 participants in which 30 were controls and 71 were cases. In the study group, mean post FEV1 was 64.45. There was a positive correlation of sputum eosinophils with severity of obstruction. The ROC curve analysis showed the cut-off value of 24.556 for serum periostin with the p-value of <0.001. As the severity of obstruction increased, the serum periostin levels were also found to be increased. Serum periostin had a sensitivity and specificity of 97.18% and 86.67% with a diagnostic accuracy of 94.06%. Conclusion: Serum periostin appears to be a more sensitive tool for detection of airflow limitation in asthmatic patients with a Th2 high eosinophilic phenotype when compared to AEC and sputum eosinophils. © 2016, Journal of Clinical and Diagnostic Research. All rights reserved.

Ramesh V.,Bharath University | Hari R.,Dr. M.G.R. Educational and Research Institute | Pandian S.,Tagore Medical College and Hospital | Arumugam G.,Bharathi Womens College
Journal of Complementary and Integrative Medicine | Year: 2011

The in-vitro free radical scavenging efficacy of the combined ethanolic Biherbal extract (BHE) from equal quantities of the leaves of Eclipta alba and seeds of Piper longum was investigated. This was compared with its individual preparation of ethanolic extract of Eclipta alba (EAE) and ethanolic extract of Piper longum (PLE). The 1, 1, diphenyl-2-picrylhydrazyl (DPPH), super oxide, nitric oxide, hydroxyl radical scavenging activity and reducing power from BHE, EAE, and PLE were investigated employing various established in vitro systems. Total phenolic and flavonoid content were also determined. The results revealed that BHE has notable activity in quenching of DPPH, super oxide, nitric oxide, and hydroxyl radicals when compared to its individual preparation of EAE and PLE. The BHE at 1000 μg/ml showed maximum scavenging of DPPH (88.75%) super oxide (84.78%) nitric oxide (89.02%) and hydroxyl (82.82%) against the scavenging of EAE and PLE which showed DPPH (81.77%,83.54%) super oxide (79.88 ± 1.84, 80.88 ± 1.44%) nitric-oxide (76.14 ± 1.80, 77.2 ± 1.18%) and hydroxyl (79.86%, 74.64%) radicals respectively at the same concentration. A linear correlation between BHE and reducing power was also observed. The quantitative estimation of the extract revealed the considerable amount of phenols and flavonoids. The results of this study strongly indicate that the BHE has more potent antioxidant potential action than its individual preparation EAE or PLE. © 2011 De Gruyter. All rights reserved.

Vaithianathan R.,ESIC MC and PGIMSR | Philipchandran,Madrs Medical College | Selvambigai G.,Chengleput Medical College | Jayaganesh P.,Tagore Medical College and Hospital
Journal of Clinical and Diagnostic Research | Year: 2013

Hepatocellular adenoma (Hca) is a rare, benign, liver cell tumour. Hca is most frequently seen in women with a history of oral contraceptive use. Hca is also reported in children with glycogen storage disorders, galactosaemia, Hurler's syndrome, severe immune deficiency states, diabetes mellitus, sex hormone disturbances, Fanconis anaemia, in those who are on androgen therapy and also in seizure disorder patients who are on carbamazepine therapy. Usually, Hca arises typically in a clinical setting of hormonal or metabolic abnormalities which stimulate hepatocyte proliferation. Though it is rare, a few cases of spontaneous Hca have been reported in children and also in adults, which were not associated with any of the known risk factors which are associated with Hca. Hca has to be differentiated from focal nodular hyperplasia, hepatocellular carcinoma and hepatoblastoma. The histological features and the assessment of cell proliferation by using immuno histochemistry, help in confirming the diagnosis of Hca. In a few cases of Hca, malignant transformations have been reported. Hence, a careful search for malignant transformations is necessary. In this report, we have documented two cases of spontaneous Hca which occurred in two normal children.

Srirangaramasamy J.,Tagore Medical College and Hospital | Srirangaramasamy J.,Second Street | Kathirvelu S.,Hopkins ENT
Indian Journal of Otolaryngology and Head and Neck Surgery | Year: 2016

Sarcoidosis is a multi systemic granulomatous disorder involving all the organs of the body. Heerfordt’s syndrome is an acute and rare presentation of sarcoidosis. It presents with fever, uveitis and bilateral parotid swelling with unilateral or bilateral facial palsy. It is seen in 0.3 % of cases of sarcoidosis. Typical presentation of Heerfordt’s syndrome is rare. Facial palsy is seen in 25–50 % of Heerfordt’s syndrome. Bilateral facial palsy is very rare contributing to only 0.3–2 % of all cases of facial nerve palsies. The diagnosis is very difficult as the facial features are less obvious compared to unilateral palsy. In this case report, we have presented a rare case of Heerfordt’s syndrome with bilateral facial palsy which was misdiagnosed as mumps. This case highlights the importance of including Heerfordt’s syndrome in evaluating the causes of facial palsy. Bilateral facial palsy unlike unilateral is less obvious. Hence a detailed history, thorough clinical examination and relevant investigations will aid in arriving at diagnosis and better management of the patient. © 2016 Association of Otolaryngologists of India

Paul S.,Tagore Medical College and Hospital
National Journal of Physiology, Pharmacy and Pharmacology | Year: 2015

Background: Chitosan (deacetylated chitin) and its derivatives, which are known to possess pharmaceutical and biomedical properties, have gained considerable attention in the biomedical field. The nontoxic, biocompatible, and biodegradable nature of chitosan makes it possible to use it for therapeutic purpose. It exhibits antibacterial effect on both Gram-positive and Gram-negative cell wall types of bacteria and so-called “antimicrobial polysaccharide.” Aims and Objective: To study the antibacterial activities of native chitosan and its derivatives against three Gram-negative bacteria (Escherichia coli, Klebsiella spp., and Proteus spp.). Materials and Methods: Bactericidal activity of native chitosan, chitosan hydrolysates obtained by hydrogen peroxide (H2O2) and lysozyme degradation, chitosan–zinc (Zn) complex, and glucosamine hydrochloride was tested against ATCC strains of E. coli, Klebsiella spp., and Proteus spp. and clinical isolates of E. coli, Klebsiella spp., and Proteus spp. procured from a clinical diagnostic laboratory. Statistical analysis was done using SPSS software, version 20. Results: Both chitosan and its derivatives markedly inhibited the growth of most bacteria tested; however, the effects differed with regard to the type of the bacterium. The minimum inhibitory concentration for E. coli ATCC strain was 50 µg and its bactericidal activity was dose dependent. Chitosan hydrolysates also exhibited an inhibitory effect, although differences were seen among strains. Degrades from H2O2 had higher activity than native chitosan. Lysozyme-degraded chitosan had less activity compared to hydrolysates obtained from H2O2 hydrolysis. Chitosan–Zn complex also showed wide spectrum of antimicrobial activities against all the microorganisms tested and the effects were found to increase with increasing chelate ratios. No antibacterial effect was observed in the case of monomer glucosamine hydrochloride, showing that only oligomers have bactericidal effect. Conclusion: The antibacterial potential of chitosan and its derivatives is considerable and its prospect to be developed as a chemotherapeutic agent is high. © 2015, Mrs Deepika Charan. All rights reserved.

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