Beierle J.M.,University of California at Irvine |
Yoshimatsu K.,University of California at Irvine |
Chou B.,University of California at Irvine |
Mathews M.A.A.,TA Instruments Waters LLC |
And 2 more authors.
Angewandte Chemie - International Edition | Year: 2014
We report a new material design concept for synthetic, thermally responsive poly(N-isopropylacrylamide)-based copolymer nanoparticle (NP) hydrogels, which protect proteins from thermal stress. The NP hydrogels bind and protect a target enzyme from irreversible activity loss upon exposure to heat but "autonomously" release the enzyme upon subsequent cooling of the solution. Incorporation of the optimized amount of negatively charged and hydrophobic comonomers to the NP hydrogels was key to achieve these desired functions. As the NP hydrogels do not show a strong affinity for the enzyme at room temperature, they can remain in solution without adversely affecting enzymatic activity or they can be removed by filtration to leave the enzyme in solution. The results demonstrate the promise of this approach for improving the thermal tolerance of proteins. Enzyme protector: Thermally responsive N-isopropylacrylamide-based polymer nanoparticle (NP) hydrogels are engineered to protect a protein from thermal stress. The NP hydrogels bind and protect a target enzyme from activity loss at elevated temperature but readily release the enzyme when the solution is cooled. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source
Lee H.-Y.,University of Maryland University College |
Diehn K.K.,University of Maryland University College |
Sun K.,University of Maryland University College |
Chen T.,TA Instruments Waters LLC |
Raghavan S.R.,University of Maryland University College
Journal of the American Chemical Society | Year: 2011
We describe a new class of photorheological (PR) fluids whose rheological properties can be reversibly tuned by light. The fluids were obtained by doping lecithin/sodium deoxycholate (SDC) reverse micelles with a photochromic spiropyran (SP) compound. Initially, the lecithin/SDC/SP mixtures formed highly viscoelastic fluids, reflecting the presence of long, wormlike reverse micelles. Under UV irradiation, the SP was isomerized to the open merocyanine (MC) form, causing the fluid viscosity to decrease 10-fold. When the UV irradiation was switched off, the MC reverted to the SP form, and the viscosity recovered its initial value. This cycle could be repeated several times without loss of response. The rheological transitions are believed to reflect changes in the lengths of the reverse worms. To our knowledge, this is the first example of a simple, reversible PR fluid that can be made entirely from commercially available components. © 2011 American Chemical Society. Source
Ta Instruments Waters L.L.C. | Date: 2014-08-22
A conditioning system includes a sample chamber capable of receiving a specimen as well as first and second specimen-holding fixtures positioned within the sample chamber and configured to apply mechanical stimulation to a specimen held by the fixtures. The system has at least one port capable of providing a fluid to the sample chamber. The chamber is sized and shaped so that it can be inserted into an imaging device which can record images of a specimen held by the fixtures.
Ta Instruments Waters L.L.C. | Date: 2014-07-31
A mechanical clamping device can include at least two contact faces, a first of the contact faces configured to travel in response to an applied force, each contact face configured to contact a sample when loaded into the mechanical clamping device. The device can further include a load element configured to cause the two contact faces to apply a clamping force to the sample when loaded into the mechanical clamping device.
TA Instruments Waters L.L.C. | Date: 2015-09-17
This document provides fluid handling components. For example, this document relates to taper interface connections such as, but not limited to, luer taper interface connections used to couple fluid handling components in a medical or laboratory setting. In some embodiments, the threaded connection on the male portion of the taper interface is comprised of one or two opposing tabs, rather than a 360 collar like conventional luer lock fittings. As such, the taper interface components provided herein are readily manufacturable with a lower cost than conventional fittings that have a collar. In some embodiments, the taper interface components provided herein are configured for coupling by way of a quick connection technique.