Tapioca and Castor Research Station

Salem Tamil Nadu, India

Tapioca and Castor Research Station

Salem Tamil Nadu, India
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Tamilselvi N.A.,Tamil Nadu Agricultural University | Pugalendhi L.,Tapioca and Castor Research Station | Sivakumar M.,Tapioca and Castor Research Station
Vegetos | Year: 2016

Pot culture experiments were conducted under in vitro to understand the biochemical changes takes place during nematode attack and to identify resistant rootstocks for grafting with bitter gourd scions. Results revealed that, three genotypes viz.,Kumatikai (Citrulus colocynthis), African horned cucumber (Cucumis metuliferus) and pumpkin (Cucurbita moschata) encompass the lowest value for number of galls per gram root, egg masses per gram root and numbers of females per gram of root and showed resistant reaction followed by two rootstocks viz.,Sponge gourd (Luffa cylindrica) and mithipakal (Momordica charantia var. muricata) bare minimum values and are moderately resistant. These resistant reaction yet again confirmed by assessment of defence enzymes viz.,phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenoloxidase (PPO), Acid phosphatase and biochemical components viz., total phenol and ortho dihydroxy phenol (OD phenol). The present study indicates that rapid augmentation of PAL, PO, PPO, acid phosphatase, total phenol and OD phenols activities were noticed in resistant rootstocks viz., C. colocynthis, C. metuliferus and C. moschata and started to increase at 24 hours after inoculation, reached maximum at 96 hours after inoculation and thereafter declined. Similarly, native poly acralamide gel electrophoresis (PAGE) analysis revealed that one to six isoforms of defence enzymes were observed in aforementioned resistant rootstocks whereas fewer isoforms with less intensity were noticed in susceptible rootstocks and bitter gourd scions. These results suggest that the aforementioned resistant rootstocks are potential source for managing root knot nematode menace in bitter gourd by grafting. © 2016, SciTechnol, All Rights Reserved.


Kalaiselvi P.,Tamil Nadu Agricultural University | Kalaiselvi P.,Tapioca and Castor Research Station | Mahimairaja S.,Tamil Nadu Agricultural University
Research on Crops | Year: 2012

The biomethanated distillery spentwash is a nutrient rich liquid organic waste obtained from molasses based distillery industries after biomethanation process. The effect of different levels and methods of spentwash application on yield attributes, yield and quality was examined through a field experiment. The field experiment was conducted using Groundnut (Arachis hypogaea L.) as a test crop. The different levels of spentwash (0, 40, 80 and 120 m 3/ha) were applied to the field in one time and continuous split doses of application. Among the spentwash applied plots, the plot which received spentwash @ 120 m 3/ ha alongwith NP recorded the maximum kernel yield of 1641 kg/ ha, oil content of 44.2% and seed crude protein of 25.1%. The continuous application of split doses of spentwash was found better than one time application. Hence, spentwash is a bioresource material which offers a low cost fertilizer alternative on agricultural utilization. In doing so, the manurial and irrigational potential of distillery spentwash invariably has a considerable economic value in the context of present energy and fertilizer nutrient crisis.

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