Mahmoud M.R.,Synthetic Organic Chemistry Laboratory |
El-Ziaty A.K.,Synthetic Organic Chemistry Laboratory |
Hussein A.M.,Synthetic Organic Chemistry Laboratory
Synthetic Communications | Year: 2013
Novel thiazolo [3,2-a]pyridine and thiazolo[3,2-a]pyrimidine derivatives, pyrazolo[3,4-b]pyrano[2,3-d]thiazole, and coumarin derivatives were synthesized from readily obtainable starting materials such as (Z)-2-ethoxycarbonyl methyl-5-(4-chlorobenzylidene)-2-thiazolidin-4(H)one 3 and (Z)-4-(4- hydroxybenzylidene)-2-imino-2-thiazolidin-4(H)one 11. © 2013 Copyright Taylor and Francis Group, LLC.
Vece V.,Synthetic Organic Chemistry Laboratory |
Vuocolo G.,Synthetic Organic Chemistry Laboratory
Tetrahedron | Year: 2015
Three novel coelenterazine derivatives substituted at the C-3 position were synthesized through a multicomponent strategy based on Groebke-Blackburn-Bienaymé reaction without using protecting groups. An efficient one-pot tert-butyl group cleavage from an aminoimidazopirazine and the first example of direct diazoimidazole derivative hydrogen abstraction in acidified water (traditional Sandmeyer hydroxylation conditions) were described. © 2015 Elsevier Ltd.
Hikone Y.,Tokyo Metroplitan University |
Hikone Y.,Synthetic Organic Chemistry Laboratory |
Hirai G.,Synthetic Organic Chemistry Laboratory |
Hirai G.,Chiyoda Corporation |
And 13 more authors.
Journal of Biomolecular NMR | Year: 2016
Structural analyses of proteins under macromolecular crowding inside human cultured cells by in-cell NMR spectroscopy are crucial not only for explicit understanding of their cellular functions but also for applications in medical and pharmaceutical sciences. In-cell NMR experiments using human cultured cells however suffer from low sensitivity, thus pseudocontact shifts from protein-tagged paramagnetic lanthanoid ions, analysed using sensitive heteronuclear two-dimensional correlation NMR spectra, offer huge potential advantage in obtaining structural information over conventional NOE-based approaches. We synthesised a new lanthanoid-chelating tag (M8-CAM-I), in which the eight-fold, stereospecifically methylated DOTA (M8) scaffold was retained, while a stable carbamidemethyl (CAM) group was introduced as the functional group connecting to proteins. M8-CAM-I successfully fulfilled the requirements for in-cell NMR: high-affinity to lanthanoid, low cytotoxicity and the stability under reducing condition inside cells. Large PCSs for backbone N–H resonances observed for M8-CAM-tagged human ubiquitin mutant proteins, which were introduced into HeLa cells by electroporation, demonstrated that this approach readily provides the useful information enabling the determination of protein structures, relative orientations of domains and protein complexes within human cultured cells. © 2016 Springer Science+Business Media Dordrecht
Al-Kahraman Y.M.S.A.,University of Balochistan |
Madkour H.M.F.,University of Balochistan |
Madkour H.M.F.,Synthetic Organic Chemistry Laboratory |
Ali D.,University of Balochistan |
Yasinzai M.,University of Balochistan
Molecules | Year: 2010
A series of eighteen azomethines has been synthesized by the reaction of appropriate primary aromatic amines with aryl and/or heteroaryl carboxaldehydes. The synthesized azomethines have been evaluated for their in vitro antileishmanial, antibacterial and antifungal activities. The results revealed some antifungal activity of most of the synthesized compounds, whereas the antileishmaniasis activity results highlighted that all synthesized azomethines inhibited parasite growth and most of them showed highly potent action towards Leishmania major promastigotes. No remarkable bactericidal activities were observed. © 2010 by the authors.