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Kanneti R.,Gujarat University | Kanneti R.,Dr Reddys Laboratories Ltd | Bhavesh D.,Synchron Research Services Pvt. Ltd | Paramar D.,Synchron Research Services Pvt. Ltd | And 2 more authors.
Biomedical Chromatography | Year: 2011

A rapid, sensitive and rugged solid-phase extraction ultraperformance liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for determination of oseltamivir phosphate (OP) and oseltamivir carboxylate (OC) in human plasma. The procedure for sample preparation includes a simple SPE extraction procedure coupled with a Chromatopack C18 column (50 × 3.0mm, i.d., 3.0μm) with isocratic elution at a flow-rate of 0.600mL /min and acyclovir was used as the internal standard. The analysis was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring mode via electrospray ionization. Using 500μL plasma, the methods were validated over the concentration ranges 0.92-745.98 and 5.22-497.49ng/mL for OP and OC, with a lower limit of quantification of 0.92and 5.22ng/mL. The intra- and inter-day precision and accuracy of the quality control samples were within 10.1%. The recovery was 68.72, 70.66 and 71.59% for OP, OC and IS, respectively. Total run time was only 1.0min. The method was highly reproducible with excellent chromatography properties. © 2010 John Wiley & Sons, Ltd.


Kanneti R.,Gujarat University | Kanneti R.,Dr Reddys Laboratories Ltd | Bhavesh D.,Synchron Research Services Pvt. Ltd | Paramar D.,Synchron Research Services Pvt. Ltd | And 2 more authors.
Biomedical Chromatography | Year: 2011

A rapid, specific and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the determination of penciclovir in human plasma. The method involved simple, one-step SPE procedure coupled with a C18, 75 × 4.mm, 3μm column with a flow-rate of 0.5mL/min, and acyclovir was used as the internal standard. The Quattro Micro mass spectrometry was operated under the multiple reaction-monitoring mode using the electrospray ionization technique. Using 250μL plasma, the methods were validated over the concentration range 52.555-6626.181ng/mL, with a lower limit of quantification of 52.55ng/mL. The intra- and inter-day precision and accuracy values were found to be within the assay variability limits as per the FDA guidelines. The developed assay method was applied to a clinical pharmacokinetic study in human volunteers. © 2010 John Wiley & Sons, Ltd.

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