Lanškroun, Czech Republic
Lanškroun, Czech Republic

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Vosatka M.,Academy of Sciences of the Czech Republic | Vosatka M.,Charles University | Latr A.,Symbiom Ltd. | Albrechtova J.,Academy of Sciences of the Czech Republic | Albrechtova J.,Charles University
Acta Horticulturae | Year: 2015

Numerous bioadditives can be applied in protected cultivation of vegetables for enhancement of their growth, yield, increase of tolerance to environmental stresses (including drought, nutrient deficiency and others) and, in particular, improving food quality of a crop. Several bioadditives' applications have been tested in three greenhouse experiments including microbial inoculation with arbuscular mycorrhizal fungi (AMF), Trichoderma harzianum and plant growth-promoting bacteria (PGPB). In addition, the effects of amendments with slow-release organic-matter-based fertilizer, an organic matter preinoculated with saprophytic fungi (SF), a solution with an algal mixture or a synthetic phytohormone-derived analogue were tested in combination with microbial treatments. Bioadditives were tested on tomato and pepper in pot experiments using horticultural substrates. Some positive responses to particular combinations of bioadditives as regards growth and/or yield were observed. In addition, parameters of increased contents of health promoting compounds were reported in some applications on tomato: content of total antioxidant capacity and Vitamin C. We suggest that the use of AMF compatible with selected strains of biocontrol fungus T. harzianum or AMF in combination with SF treated organic matter or organic matter-based fertilizer can be successfully used in production of tomatoes and other vegetable crops in particular in protected horticultural cultivation in soilless substrates. The use of bioadditives in commercial vegetable production offers an ecological way of cultivation while possibly improving food quality and health promoting properties of vegetable crops based on increased accumulation of healthpromoting phytochemicals. Bioadditives can be an integral beneficial part of ecological, organic vegetable production.


Vosatka M.,Academy of Sciences of the Czech Republic | Latr A.,Symbiom Ltd. | Gianinazzi S.,InoculumPlus Ltd. | Albrechtova J.,Academy of Sciences of the Czech Republic | Albrechtova J.,Charles University
Symbiosis | Year: 2012

Advanced scientific knowledge on arbuscular mycorrhizal symbioses recently enhanced potential for implementation of mycorrhizal biotechnology in horticulture and agriculture plant production, landscaping, phytoremediation and other segments of the plant market. The advances consist in significant findings regarding: - new molecular detection tools for tracing inoculated fungi in the field; - the coexistence mechanisms of various fungi in the single root system; - new knowledge on in vitro physiology of the AM fungi grown in root organ cultures; - mechanisms of synergistic interactions with other microbes like PGPR or saprotrophic fungi; discovery of mycorrhiza supportive compounds such as strigolactones. Scientific knowledge has been followed by technological developments like novel formulations for liquid applications or seed coating, mycorrhiza stimulating compounds or new application modes. Still the missing components of biotechnology are appropriate, cheap, highly reproducible and effective methods for inocula purity testing and quality control. Also there is a weak traceability of the origin of the mycorrhizal fungi strains used in commercial inocula. Numerous poor quality products can still be found on the markets claiming effective formation mycorrhiza which have very low capacity to do so. These products usually rely in their effects on plant growth not on support of host plants via formation of effective mycorrhizal symbiosis but on fertilizing compounds added to products. There is growing number of enterprises producing mycorrhiza based inocula recently not only in developed world but increasingly in emerging markets. Also collaboration between private sector and scientific community has an improving trend as the development of private sector can fuel further research activities. Last but not least there is apparent growing pull of the market and increasing tendency of reduction of agrochemical inputs and employment of alternative strategies in planting and plant production. These circumstances support further developments of mycorrhizal inocula production and applications and maturation of the industry. © 2013 Springer Science+Business Media Dordrecht.


Hernadi I.,Szent Istvan University | Sasvari Z.,Szent Istvan University | Albrechtova J.,Charles University | Albrechtova J.,Academy of Sciences of the Czech Republic | And 3 more authors.
HortScience | Year: 2012

Although the majority of horticultural crops are mycorrhiza-dependent, the role of arbuscular mycorrhizal (AM) inoculation in plant production has been neglected in high-input agriculture. Field application of a commercial inoculum mix of Glomus spp. was tested in spice pepper (Capsicum annuum L. var. longum), cv. Szegedi, cultivation. With polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP), differences in small subunit ribosomal RNA genes were used to characterize groups of arbuscular mycorrhizal fungi (AMF) with respect to effects of mycorrhizal inoculation on an indigenous AMF population. The AMF inoculant was able to establish in the rhizosphere of pepper plants and mycorrhizal inoculation increased yield of spice pepper by more than 65% compared with the non-treated control plants. Having relatively high root colonization in the control, non-inoculated treatment indicated high presence of indigenous populations of AMF in the field soil. Although the inoculation affected structure of the resident AM fungal community, it did not influence the composition of AMF associated with pepper roots significantly.


Albrechtova J.,Charles University | Albrechtova J.,Academy of Sciences of the Czech Republic | Latr A.,Symbiom Ltd. | Nedorost L.,Mendel University in Brno | And 4 more authors.
The Scientific World Journal | Year: 2012

The aim of this paper was to test the use of dual microbial inoculation with mycorrhizal and saprotrophic fungi in onion cultivation to enhance yield while maintaining or improving the nutritional quality of onion bulbs. Treatments were two-factorial: (1) arbuscular mycorrhizal fungi (AMF): the mix corresponding to fungal part of commercial product Symbivit (Glomus etunicatum, G. microaggregatum, G. intraradices, G. claroideum, G. mosseae, and G. geosporum) (M1) or the single-fungus inoculum of G. intraradices BEG140 (M2) and (2) bark chips preinoculated with saprotrophic fungi (mix of Gymnopilus sp., Agrocybe praecox, and Marasmius androsaceus) (S). The growth response of onion was the highest for the M1 mix treatment, reaching nearly 100% increase in bulb fresh weight. The effectiveness of dual inoculation was proved by more than 50% increase. We observed a strong correlation (r=0.83) between the growth response of onion bulbs and AM colonization. All inoculation treatments but the single-fungus one enhanced significantly the total antioxidant capacity of bulb biomass, was the highest values being found for M1, S + M1, and S + M2. We observed some induced enhancement of the contents of mineral elements in bulb tissue (Mg and K contents for the M2 and M2, S, and S+M2 treatments, resp.). © 2012 Jana Albrechtova et al.

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