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Sydney, Australia

Bradley C.K.,Sydney IVF Stem Cells | Chami O.,Sydney IVF Stem Cells | Peura T.T.,Sydney IVF Stem Cells | Peura T.T.,University of Queensland | And 5 more authors.
In Vitro Cellular and Developmental Biology - Animal

Human embryonic stem cells are pluripotent cells capable of extensive self-renewal and differentiation to all cells of the embryo proper. Here, we describe the derivation and characterization of three Sydney IVF human embryonic stem cell lines not already reported elsewhere, designated SIVF001, SIVF002, and SIVF014. The cell lines display typical compact colony morphology of embryonic stem cells, have stable growth rates over more than 40 passages and are cytogenetically normal. Furthermore, the cell lines express pluripotency markers including Nanog, Oct4, SSEA3 and Tra-1-81, and are capable of generating teratoma cells derived from each of the three germ layers in immunodeficient mice. These experiments show that the cell lines constitute pluripotent stem cell lines. © 2010 The Society for In Vitro Biology. Source

Bradley C.K.,Sydney IVF Stem Cells | Scott H.A.,Sydney IVF Stem Cells | Chami O.,Sydney IVF Stem Cells | Peura T.T.,Sydney IVF Stem Cells | And 3 more authors.
Stem Cells and Development

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by an expansion of cytosine-adenine-guanine (CAG) repeats in the Huntingtin gene Htt. To facilitate research into HD, we have derived 4 human embryonic stem cell (hESC) lines containing ≥40CAG repeats in exon 1 of Htt: SIVF017-HD (CAG 40), SIVF018-HD (CAG 46), SIVF020-HD (CAG 48), and SIVF046-HD (CAG 45). Additionally, we have derived a normal sibling-matched control for SIVF020-HD, cell line SIVF019. All 5 hESC lines had a normal karyotype, expressed pluripotency markers including Oct4, SSEA3, and Tra-1-81, and could be maintained in culture for multiple (>40) passages. Teratoma studies revealed that the hESC lines were capable of differentiating into cells representative of the 3 germ layers. Furthermore, in vitro neuronal differentiation experiments have confirmed that the hESC lines were able to generate MAP2-positive neuronal cells that express the Htt protein. Combined, these experiments confirm that the cell lines represent pluripotent stem cell lines. These HD-affected hESC lines will be made available to biomedical research laboratories and will provide a valuable tool to investigate the mechanisms and potential treatments for HD. © 2011, Mary Ann Liebert, Inc. Source

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