Ehrs S.,National Veterinary Institute |
Ehrs S.,Swedish Laboratory for Food Safety and Biopreparedness |
Agren P.,National Food Administration |
Agren P.,Swedish Laboratory for Food Safety and Biopreparedness |
And 6 more authors.
Journal of Food Safety | Year: 2011
A high-throughput biosafety level 3 suitable method is necessary for rapid discovery of unknown pathogens in complex matrices from the food chain. In this paper, three automated bacterial DNA extraction methods for real-time polymerase chain reaction (PCR) detection were evaluated. The systems compared were the Magnatrix (NorDiag, Oslo, Norway) and the EZ1 (Qiagen, Hilden, Germany), both based on purification with magnetic beads, and the ABI Prism™ 6100 Nucleic Acid PrepStation (Applied Biosystems, Foster City, CA), which is based on silica gel filtration. In the comparison, the detection of bacterial DNA from food and feed seeded with one Gram-positive and one Gram-negative bacterial species was compared. A wider range of matrices were successfully processed using the magnetic bead-based DNA extraction robots compared with the silica gel filtration-based robot. The limit of detection was 102-103cfu/g matrix of both bacteria in powdered milk, minced meat, whole wheat and soypass using the EZ1 extraction robot. © 2011 Wiley Periodicals, Inc.