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Indore, India

Eswara Reddy N.P.,Sv Agricultural College | Basha S.T.,Andhra University
Research Journal of Biotechnology | Year: 2013

A study was focused on the interaction of Erwinia chrysanthemi with Arabidopsis thaliana C24. Erwinia chrysanthemi has shown compatible interaction and caused soft rot by complete maceration of the tissues and collapse of the infected plant part. Inductionof random β-glucuronidase (GUS) gene fusions in transgenic Arabidopsis after infection with E. chrysanthemi was observed. Transgenic Arabidopsis lines At1082 and At1275 have shown increased GUS expression after infection with E. chrysanthemi. Histochemicalstaining results indicated localized GUS expression in the vascular bundles of the leaves present above the site of infection. DNA analysis results showed the presence of a single T-DNA copy in transgenic lines At1085 and At1275. The possibility of cloning E. chrysanthemi induced A. thaliana plant promoter sequences is discussed.

Hariprasad K.V.,University of Reading | Hariprasad K.V.,Sv Agricultural College | van Emden H.F.,University of Reading
International Journal of Pest Management | Year: 2010

Artificial diet studies were used to differentiate among physical and chemical mechanisms affecting the suitability to diamondback moth (Plutella xylostella L.), of 16 food substrates obtained by growing four different brassicas in the glasshouse or field and measuring the pest's performance on either leaf discs or a diet incorporating leaf powders. Leaves of Chinese cabbage and the cabbage cultivar 'Minicole' were, respectively, the most and least suitable leaves for the insect, but this ranking was reversed on artificial diet. Leaves of glasshouse-grown plants were more suitable than those of plants grown in the fields. Differences in the suitability of leaves to diamondback moth appeared to be largely determined by leaf toughness and surface wax load. Concentrations of individual glucosinolates in the brassicas probably acted as phagostimulants, so increasing their intrinsic susceptibility to diamondback moth, but the effect of the physical factors appeared more important. © 2010 Taylor & Francis.

Kalyani D.L.,Sv Agricultural College
Legume Research | Year: 2012

A field experiment was conducted at S.V. Agricultural College, Tirupati, to study the effect of different sowing dates on the seed yield and quality of different varieties of guar. The experiment was laid out in a factorial randomized block design replicated thrice. Four dates of sowing viz., first and second fortnight of July and first and second fortnight of August. Genotypes are RGC 1003, HG 563, RGM 112 and GAUG 9703. The results were revealed that growth parameters, yield attributes, yield and quality parameters were highest with RGM 112 sown during first fortnight of July, which was at par with HG 563 sown at the same time.

Madhumathi C.,Andhra University | Reddi Sekhar M.,Andhra University | Reddi Sekhar M.,Sv Agricultural College
Indian Journal of Horticulture | Year: 2015

Genetic variation in 27 sweet orange accessions was undertaken at Citrus Research Station (AICRP on Tropical Fruits), Tirupati with three replications of one tree in each accession. The observations on 32 quantitative morphological characters with respect to plant, fruit and quality characters were done following IPGRI descriptors. High GCV and PCV were recorded for seed weight (GCV = 67.24%; PCV = 74.79%) and average number of seeds per fruit (GCV = 33.83%; PCV = 34.16%). Moderate PCV and GCV values were reported for rind thickness (GCV = 19.61%; PCV = 20.39%), width of epicarp (GCV = 17.95%; PCV=20.38%), diameter of fruit axis (GCV= 15.02%; PCV = 15.61%) and fruit weight (GCV = 12.64%; PCV = 13.40%) among fruit characters. The high heritability associated with high genetic advance was observed in petiole length, leaf lamina width, anther length, pedicel length, stamen length, petal length, diameter of fruit axis, rind thickness, fruit weight, width of epicarp, average number of seeds per fruit, seed width, seed weight, ascorbic acid content, titrable acidity, total sugars, reducing sugars and ratio of soluble solids to titrable acids indicating the scope for improving these traits through selection. © 2015 Horticulture Society of India. All rights reserved.

Nagendra Prasad B.,Sv Agricultural College | Reddi Kumar M.,Sv Agricultural College | Reddi Kumar M.,Acharya N.G. Ranga Agricultural University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Cultural and morphological characteristics of sheath blight pathogen; Rhizoctonia solani was observed on different solid media viz., potao dextrose agar (PDA) medium, czapeck's dox agar (CDA) medium and rose bengal agar (RBA) medium. On PDA, mycelial growth was abundant, but sclerotia production was delayed. On CDA, though the mycelial growth was moderate and slower than on PDA, sclerotia production was early. On both the media, the sclerotia were aggregated. The mycelium growth was very slow on RBA and there was no sclerotia production. Scanning Electron Microscopy (SEM) of hyphal interaction between Trichoderma spp. and R. solani indicated that biocontrol agent parasitized the mycelium of R. solani. Hypha of R. solani was highly susceptible to hyphal parasitic attack by the species of Trichoderma. Studies of hyphal interaction between Trichoderma and R. solani indicates penetration of parasite and finally resulting into lysis or collapse of R. solani hypha. Among the Trichoderma spp. TK3 isolate showed more mycoprasitic activity by making contact with host hyphae, running parallel to it, production of hook like structures and emptied the cells of pathogen.

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