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Zhang X.,The General Hospital of PLA | Zheng H.,Allergy and Inflammation Research Institute | Zhang H.,Suzhou Xiangcheng Peoples Hospital | Ma W.,Nanjing Medical University | And 3 more authors.
Cytokine | Year: 2011

Recently, involvement of IL-17 in development of COPD has been noticed. Unlike IL-8, the role of IL-17 in COPD remains controversial. In order to further understand mechanisms in cigarette smoke (CS) induced COPD, we investigated IL-17 and IL-8 levels in different stages of COPD patients, and time courses of IL-17 and IL-8 release in CS induced COPD rats. A total of 73 elderly patients with COPD and 31 healthy volunteers were recruited in the study. IL-17 and IL-8 levels in the sputum and plasma were measured, and number of differential cells was counted. A newly developed CS induced rat COPD model was employed to study time courses of IL-17 and IL-8 release and nucleated cell accumulation. The results showed that IL-8 levels in the sputum of severe COPD patients were elevated by 16.5-fold, but IL-17 levels were reduced by 4.8-fold. While IL-8 correlated with neutrophils, IL-17 correlated with monocytes and lymphocytes. Similarly, level of IL-8 was increased, but IL-17 was decreased in the bronchoalveolar lavage fluid (BALF) of CS rats. Time course study showed that increased IL-8 production in the BALF initiated at 6. weeks, but decreased IL-17 production started at 10. weeks following CS exposure. In conclusion, increased IL-8 level in COPD patients appears mainly secreted from neutrophils and macrophages, whereas decreased IL-17 level seems resulted from reduced number of monocytes or damaged epithelial cells. Increased IL-8 (a proinflammatory cytokine) secretion and decreased IL-17 (a protective cytokine of airways) release can both contribute to development of COPD. © 2011 Elsevier Ltd.


PubMed | Suzhou Xiangcheng Peoples Hospital, Nanjing Medical University, Precision for Medicine, Xuzhou Central Hospital and Soochow University of China
Type: Journal Article | Journal: Oncology letters | Year: 2016

The aim of the study was to investigate the application value of neutrophil to lymphocyte ratio (NLR) and the platelet to lymphocyte ratio (PLR) in the prediction of chemotherapy response and prognosis in patients with advanced gastric cancer. In total, 120 patients with unresectable gastric cancer were included and separated into two groups according to the median values of NLR or PLR (NLR low: <4.62 or NLR high: 4.62 and PLR low: <235 or PLR high: 235, respectively). Low baseline NLR level correlated with improved clinicopathological characteristics, including smaller tumor size, well differentiation and less metastasis. Low baseline PLR level also associated with less metastasis. Patients with a low baseline level of NLR or PLR had an improved response to chemotherapy. Patients with a higher baseline NLR and PLR had decreased progression-free survival (PFS) and overall survival (OS) ratios. Alterations in the NLR and PLR levels were associated with therapeutic efficacy and prognosis. The patients who remained in or switched to the low NLR level subgroup subsequent to first-line chemotherapy had an improved response and improved OS ratios, compared to the patients remaining in or switching to the high NLR level group. Similar results were observed when the PLR level was investigated. In conclusion, baseline NLR and PLR measurements, as well as changes of NLR and PLR following chemotherapy can predict the prognostic results in patients with unresectable gastric cancer.


PubMed | Suzhou Xiangcheng Peoples Hospital, Nanjing Medical University, Xuzhou Central Hospital and Soochow University of China
Type: Journal Article | Journal: Oncology letters | Year: 2016

Gastric cancer is the fourth most frequent cancer and the second cause of cancer-related mortalities worldwide. Platelets play an important and multifaceted role in cancer progression. Elevated mean platelet volume (MPV) detected in peripheral blood has been identified in various types of cancer. In the present study, we investigated the application value of MPV in the prediction of chemotherapy response and prognosis in patients with unresectable gastric cancer. A total of 128 patients with unresectable gastric cancer were included and divided according to the median values of baseline MPV (low MPV: <11.65 or high MPV: 11.65). A low baseline MPV level was correlated with reduced metastasis. The results showed that patients with a low baseline level of MPV improved response to chemotherapy. Changes in MPV were associated with therapeutic efficacy. Patients who remained in or were transferred into the low MPV level subgroup following first-line chemotherapy had improved response, compared to those remaining in or being transferred into the high MPV level group. The patients with a higher baseline MPV had decreased progression-free and overall survival ratios. Univariate and multivariate analyses revealed that baseline MPV was a prognostic factor affecting progression-free survival. In conclusion, the results showed that MPV measurements can provide important prognostic information for gastric cancer patients.


Wang Y.,Nanjing Medical University | Wang Y.,Fudan University | Li D.,Fudan University | Xu N.,Nanjing Medical University | And 7 more authors.
Arthritis Research and Therapy | Year: 2011

Introduction: Follistatin-like protein 1 (FSTL1) is a secreted glycoprotein that has been implicated in arthritis pathogenesis in a mouse model. The aim of this study is to detect FSTL1 expression and to further assess its potential utility as a biomarker of joint damage in osteoarthritis (OA) patients.Methods: FSTL1 expression was detected by real-time PCR, western blot and immunohistochemistry (IHC) in the synovial tissues (STs) and by IHC in the articular cartilage from OA patients and control trauma patients. The serum and synovial fluid (SF) FSTL1 concentrations were measured by ELISA in OA patients and control individuals. Linear regression analyses were used to assess correlations between the serum FSTL1 levels and the clinical characteristics in OA patients.Results: The FSTL1 mRNA and protein levels were substantially elevated in the STs from OA patients compared with those from control trauma patients. The FSTL1 expression was strong in the cytoplasm of the synovial and capillary endothelial cells of the STs, but weak in the chondrocytes of the articular cartilage from OA patients. Furthermore, the serum and SF FSTL1 concentrations were significantly higher in OA patients than in respective control subjects. Interestingly, the serum and SF FSTL1 levels were markedly higher in female OA patients than in males. Importantly, bivariate regression analysis revealed that the serum FSTL1 levels in female OA patients had significant correlations with Kellgren and Lawrence (KL) grade, joint space narrowing (JSN) and the Western Ontario McMaster and Universities Osteoarthritis (WOMAC) stiffness subscale, an inverse correlation with height, and marginal correlations with the total WOMAC score and the WOMAC function subscale. Multivariate regression analysis revealed that the serum FSTL1 levels correlated independently with KL grade in female OA patients. Bivariate analysis also revealed that the serum FSTL1 levels correlated significantly with age and disease duration, and they correlated marginally with high sensitivity C-reactive protein (hs-CRP) and KL grade in male OA patients.Conclusions: Increased FSTL1 expression may be a characteristic of OA patients. FSTL1 is a potential serum biomarker that may reflect the severity of joint damage, and further studies are required to evaluate its potential application for monitoring the course of the disease and the efficacy of therapies in OA patients. © 2011 Wang et al.; licensee BioMed Central Ltd.


Xu H.-G.,Suzhou Xiangcheng Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: For younger patients with intertrochanteric fractures, many fixation methods can provide an effective and robust fixation, but for elderly patients with osteoporosis intertrochanteric fracture, there remains debate for which fixation method is optimum. OBJECTIVE: To compare the clinical outcomes of proximal femoral nail anti-rotation and dynamic hip screw in the repair of intertrochanteric fracture. METHODS: Clinical data of patients with intertrochanteric fracture treated by proximal femoral nail anti-rotation or dynamic hip screw at the Suzhou Xiangcheng People’s Hospital from January 2010 to January 2013 were selected. In accordance with the inclusion criteria, 140 patients (146 hips) were included, containing 70 patients in the proximal femoral nail anti-rotation group (74 hips) and 70 patients in the dynamic hip screw group (72 hips). No significant difference in preoperative general data was detected in patients of both groups (P > 0.05), showing a comparability. Operative time, intra-operative blood loss, the average length of hospital stay, postoperative ambulation time, perioperative hemoglobin loss amount, postoperative complications, healing time, and Harris score were compared and evaluated in both groups. RESULTS AND CONCLUSION: 140 patients were followed up for 8-16 months. The incision was stage I healing. Compared with dynamic hip screw group, operative time, intra-operative blood loss, the average length of hospital stay, and postoperative ambulation time were better in the proximal femoral nail anti-rotation group (P < 0.05). However, no significant differences in perioperative hemoglobin loss amount, healing time, and postoperative Harris score were visible in the both groups (P > 0.05). These findings suggested that proximal femoral nail anti-rotation and dynamic hip screw in the repair of intertrochanteric fracture could obtain satisfactory clinical outcomes, but proximal femoral nail anti-rotation has some advantages such as short operative time, less blood loss, short mean hospital stay, early postoperative ambulation time and less postoperative complications. © 2014 Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Guo L.F.,Suzhou Xiangcheng Peoples Hospital | Wang Z.H.,Suzhou Xiangcheng Peoples Hospital | Wang Y.F.,Suzhou Xiangcheng Peoples Hospital
Genetics and Molecular Research | Year: 2016

As a heterogeneous group of disorders in pregnancy, many genetic factors are involved in the development of preeclampsia. The single nucleotide polymorphism (SNP) rs7579169, located on chromosome 2q14.2, has been shown to be associated with pregnancy-induced hypertension in Europeans. In this study, we examined whether the SNP rs7579169 is associated with the susceptibility to preeclampsia through a case-control research model in Han Chinese women. Genotypes of 145 patients with preeclampsia and 150 healthy pregnant subjects were identified by direct sequencing. The correlation between the rs7579169 genotype and the susceptibility to preeclampsia was evaluated using an unconditional logistic regression model. Although there were no differences of having the rs7579169 SNP between early onset and late onset preeclampsia, patients carrying the CT or TT genotype were more likely to develop preeclampsia than those carrying the CC genotype (CT vs CC: OR = 1.76, 95%CI = 1.07-2.87, P < 0.05; TT vs CC: OR = 5.03, 95%CI = 1.99-12.73, P < 0.05; CC vs CT + TT: OR = 2.05, 95%CI = 1.27-3.30, P < 0.05). In conclusion, although no differences of the rs7579169 SNP were identified between the early onset and late onset preeclampsia groups, we found that the CT or TT genotype and the CT+TT genotype were significantly associated with an increased risk of preeclampsia in Han Chinese women. © UNPEC-RP.


Zhang H.,Liaoning Medical University | Zhang H.,Suzhou Xiangcheng Peoples Hospital | Kong H.,Nanjing Medical University | Zeng X.,Nanjing Medical University | And 3 more authors.
Journal of Translational Medicine | Year: 2014

In recent years, it is recognized that acquired immunity is controlled by regulatory T cell (Treg). Since fundamental pathophysiological changes of allergy are mainly caused by hyperresponsiveness of immune system to allergens that acquires after birth, Tregs likely play key roles in the pathogenesis of allergy, particularly during the sensitization phase. However, accumulated information indicate that there are several distinctive subtypes of Tregs in man, and each of them seems to play different role in controlling immune system, which complicates the involvement of Tregs in allergy. The aim of the present study is to attempt to classify subtypes of Tregs and summarize their roles in allergy. Tregs should include natural Tregs (nTreg) including inducible costimulator (ICOS)(+) Tregs, inducible/adaptive Tregs (iTreg), interleukin (IL)-10-producing type 1 Tregs (Tr1 cells), CD8(+) Tregs and IL-17-producing Tregs. These cells share some common features including expression of Foxp3 (except for Tr1 cells), and secretion of inhibitory cytokine IL-10 and/or TGF-β. Furthermore, it is noticeable that Tregs likely contribute to allergic disorders such as dermatitis and airway inflammation, and play a crucial role in the treatment of allergy through their actions on suppression of effector T cells and inhibition of activation of mast cells and basophils. Modulation of functions of Tregs may provide a novel strategy to prevent and treat allergic diseases. © 2014 Zhang et al.; licensee BioMed Central Ltd.


You F.J.,Suzhou Xiangcheng Peoples Hospital | Shen D.M.,Suzhou Xiangcheng Peoples Hospital
Genetics and Molecular Research | Year: 2016

Insertion/deletion (I/D) polymorphisms of the gene encoding angiotensin converting enzyme (ACE) are a controversial risk factor for heart diseases (HDs). ACE I/D polymorphism has been reported to be associated with various cardiovascular diseases. However, some studies have presented conflicting results. In this study, we aim to explore the association between ACE I/D polymorphisms and the risk of coronary HD (CHD), coronary artery disease (CAD), and myocardial infarction (MI). A meta-analysis was conducted, which included 12,533 cases and 20,726 controls from 75 case-control studies. We performed overall analysis on the entire dataset and found that the D allele of ACE was significantly associated with increased risk of HDs in three different comparison models (dominant, recessive, and homozygote). We also performed analyses on subgroups based on ethnicity as well as disease type. Our results showed that the D allele of ACE was significantly associated with an increased risk of HDs in the Asian and European groups but not in the American group. In addition, in all three subgroups (CHD, CAD, and MI), the D allele of ACE was found to be significantly associated with increased risk of disease. Begg’s funnel plots were generated to evaluate publication biases, but no obvious publication bias was found in the studies included in our metaanalysis. In conclusion, our meta-analysis demonstrated that the D allele of ACE was significantly associated with an increased risk of HDs. © FUNPEC-RP.


PubMed | Suzhou Xiangcheng Peoples Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

As a heterogeneous group of disorders in pregnancy, many genetic factors are involved in the development of preeclampsia. The single nucleotide polymorphism (SNP) rs7579169, located on chromosome 2q14.2, has been shown to be associated with pregnancy-induced hypertension in Europeans. In this study, we examined whether the SNP rs7579169 is associated with the susceptibility to preeclampsia through a case-control research model in Han Chinese women. Genotypes of 145 patients with preeclampsia and 150 healthy pregnant subjects were identified by direct sequencing. The correlation between the rs7579169 genotype and the susceptibility to preeclampsia was evaluated using an unconditional logistic regression model. Although there were no differences of having the rs7579169 SNP between early onset and late onset preeclampsia, patients carrying the CT or TT genotype were more likely to develop preeclampsia than those carrying the CC genotype (CT vs CC: OR = 1.76, 95%CI = 1.07-2.87, P < 0.05; TT vs CC: OR = 5.03, 95%CI = 1.99-12.73, P < 0.05; CC vs CT + TT: OR = 2.05, 95%CI = 1.27-3.30, P < 0.05). In conclusion, although no differences of the rs7579169 SNP were identified between the early onset and late onset preeclampsia groups, we found that the CT or TT genotype and the CT+TT genotype were significantly associated with an increased risk of preeclampsia in Han Chinese women.


PubMed | Suzhou Xiangcheng Peoples Hospital, Saskatchewan Cancer Agency and Soochow University of China
Type: Journal Article | Journal: Cancer gene therapy | Year: 2016

Tumor necrosis factor (TNF)--induced protein 8-like 2 (TNFAIP8L2/TIPE2) as a novel anti-inflammatory factor plays an important role in maintaining immune homeostasis. Recently, TIPE2 has been shown to inhibit hepatocarcinoma growth and metastasis through targeting Ras and Rac1. However, its effects in human cancers are poorly understood. In the present study, we analyzed TIPE2 mRNA expression in a panel of human gastric cancer cells (AGS, HGC-27 and SGC-7901) and then examined the cell-autonomous effects of adenovirus-mediated human TIPE2 gene transfer (AdVTIPE2) on AGS and HGC-27 human gastric cancer cells. We found that compared with the GES-1 normal human gastric mucous epithelial cells, human TIPE2 was lost in the AGS, HGC-27 and SGC-7901 gastric cancer cells. Adenovirus-mediated human TIPE2 overexpression significantly inhibited AGS and HGC-27 gastric cancer cell growth and induced AGS and HGC-27 tumor cell apoptosis in vitro. Furthermore, AdVTIPE2 treatment obviously suppressed the growth of AGS gastric cancer subcutaneously xenografted tumors implanted in athymic BALB/c nude mice in vivo. Mechanistically, AdVTIPE2 exhibited marked effects on the upregulation of Bax, cleaved Caspase-9, cleaved Caspase-3, cleaved poly ADP ribose polymerase as well as the downregulation of B-cell lymphoma (Bcl)-XL, phosphorylated-protein kinase B (p-PKB/AKT), phosphorylated-extracellular signal-regulated kinase 1/2 (p-ERK1/2) in AGS gastric cancer cells in vitro and in vivo. Collectively, AdVTIPE2 suppressed gastric cancer growth very possibly by the activation of intrinsic apoptotic pathway and the attenuation of AKT and ERK1/2 signaling. Thus, our data indicated that TIPE2 may be a novel potential therapeutic target for human gastric cancer.

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